Fren_00110 Fren_00110   Fren_00130 Fren_00130

Fren_00120 : CDS information

close this sectionLocation

Organism
StrainAK24158
Entry nameFrenolicin
Contig
Start / Stop / Direction14,336 / 15,613 / + [in whole cluster]
14,336 / 15,613 / + [in contig]
Location14336..15613 [in whole cluster]
14336..15613 [in contig]
TypeCDS
Length1,278 bp (425 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase beta-ketoacyl synthase subunit
Product (GenBank)ketoacyl synthase
GenefrnL
fren-ORF1
Gene (GenBank)frnL
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
  • FrnL; ORF1 of Bibb et al
Reference
ACC
PmId
[8181754] Cloning, sequencing and deduced functions of a cluster of Streptomyces genes probably encoding biosynthesis of the polyketide antibiotic frenolicin. (Gene. , 1994)
comment
[PMID:8181754](1994)
frenolicin biosynthesis gene clusterにある6ORFの同定。

ORF1: PKS beta-ketoacyl synthase(condensing enzyme)
perhaps carries an acytransferase site

たいてい見られるKSとCLFのoverlapなし。

---
上記論文のref論文1993bでfren minimal genesをact minimal genesと組み合わせてpolyketide形成を検討している。

[PMIDなし]Engineered biosynthesis of novel polyketides: manipulation and analysis of an aromatic polyketide synthase with unproven catalytic specificities. J. Am. Chem. Soc., 1993
Related Reference
ACC
Q54495
NITE
Nogl_00370
PmId
[8668120] A gene cluster involved in nogalamycin biosynthesis from Streptomyces nogalater: sequence analysis and complementation of early-block mutations in the anthracycline pathway. (Mol Gen Genet. , 1996)
comment
Blast 10th, id72%, 1e-167
Streptomyces nogalater_snoa1
Snoa1
[DoBISCUIT]polyketide synthase beta-ketoacyl synthase subunit

S. nogalaterのanthracycline生合成gene clusterを解析。
11kb領域に11ORFs(snoEDCBA123XYZ)が同定されている。

sno1: KS, AT

aclacinomycin生合成がブロックされたS.galilaeus mutantsの相補によりminimal PKSを確認している。

close this sectionPKS/NRPS Module

KS3..375

close this sectionSequence

selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [ketoacyl synthase]
MNRQVAVTGIGVVAPGGIGRKPYWEQLTSGRTATRAISFFDASPFRSRIAAEVDFDPAAA
GLSPREVRRMDRAAQFAVVSARESLADSGLDVADLDPHRIGVSIGSAVGGTTSLEREYLA
LSDSGRQWELDLSYLSPHLYDAFTPSSLAREVAGVIGAEGPAAVVSTGCTSGIDSLGHAR
DLIAEGSADVVLAGGTDTPISPIAVACFDAIKATSPSNDDPAHASRPFDRERNGFVLAEG
AAVLVLEELGHARARDAHVYALVSGYATRCNAYHMTGLTPHGREMAEAIRHALAESGTDP
AAVDYVNAHGSGTKQNDRHETAAFKATLGERARSVPVSSIKSMIGHSLGAIGSLEIAAWA
LAMEYGVVPPTANLDTPDPECDLDYVPHEARETRVDRVLSVGSGFGGFQSAMVLTRDTGA
RLPTA
selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [ketoacyl synthase]
GTGAACCGACAAGTCGCCGTCACCGGCATCGGCGTGGTGGCTCCGGGAGGGATCGGGCGC
AAGCCGTACTGGGAGCAGCTCACCTCCGGACGCACCGCCACGCGCGCCATCTCCTTCTTC
GACGCCTCGCCCTTCCGCTCGCGGATCGCCGCCGAGGTCGACTTCGACCCCGCGGCGGCG
GGCCTGAGCCCGCGCGAGGTCCGCCGCATGGACCGGGCCGCGCAGTTCGCCGTCGTGAGC
GCCAGGGAGAGCCTCGCCGACAGCGGACTCGACGTCGCCGACCTCGACCCCCACCGGATC
GGGGTGAGCATCGGCAGCGCCGTGGGCGGGACCACCTCGCTGGAACGCGAGTACCTCGCC
CTCAGCGACAGCGGGCGACAGTGGGAACTCGACCTCTCCTACCTCTCGCCGCACCTCTAC
GACGCCTTCACCCCCAGCTCGCTCGCCCGCGAGGTGGCCGGGGTGATCGGCGCGGAGGGG
CCCGCGGCAGTCGTCTCCACCGGCTGCACCTCCGGCATCGACTCGCTCGGCCACGCCCGC
GACCTCATCGCCGAGGGCAGCGCCGACGTCGTGCTCGCGGGCGGCACCGACACCCCCATC
TCGCCGATCGCCGTCGCCTGCTTCGACGCCATCAAGGCCACCTCGCCCAGCAACGACGAC
CCGGCGCACGCCTCGCGCCCCTTCGACAGGGAACGCAACGGCTTCGTCCTCGCGGAGGGC
GCCGCCGTCCTCGTGCTGGAGGAGCTGGGGCACGCGCGGGCCCGCGACGCCCACGTCTAC
GCTCTCGTGTCCGGCTACGCCACGCGCTGCAACGCGTACCACATGACCGGGCTCACCCCG
CACGGCAGGGAGATGGCCGAGGCGATCCGGCACGCGCTCGCCGAGAGCGGGACCGACCCC
GCCGCCGTGGACTACGTCAACGCCCACGGCTCCGGCACCAAGCAGAACGACCGGCACGAG
ACCGCCGCCTTCAAGGCCACGCTCGGCGAGCGGGCCAGGAGCGTGCCGGTCAGCTCCATC
AAGTCGATGATCGGCCACTCGCTCGGTGCCATCGGTTCGCTGGAGATCGCGGCCTGGGCG
CTCGCCATGGAGTACGGCGTGGTGCCGCCCACCGCCAACCTCGACACGCCCGACCCCGAG
TGCGACCTCGACTACGTGCCCCACGAGGCGCGCGAGACGCGGGTGGACAGGGTCCTGAGC
GTCGGCAGCGGCTTCGGCGGTTTCCAGAGCGCCATGGTCCTCACCCGGGACACGGGCGCC
CGCCTCCCGACGGCCTGA
KS3..375
KS7..1125

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[3-250] 1.6e-61 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[260-375] 2.50000000000001e-39 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[7-264] 1.10000000000001e-67 G3DSA:3.40.47.10 [270-416] 1.09999999999999e-54 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[2-257] 2.0000139277205e-69 SSF53901 [219-416] 2.89997807423187e-66 SSF53901
SSF53901   Thiolase-like
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[160-176] PS00606
PS00606   B_KETOACYL_SYNTHASE
SignalP
[1-19] 0.113 Signal
Eukaryota   
TMHMM No significant hit
Fren_00110 Fren_00110   Fren_00130 Fren_00130
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