Fren_00120 Fren_00120   Fren_00140 Fren_00140

Fren_00130 : CDS information

close this sectionLocation

Organism
StrainAK24158
Entry nameFrenolicin
Contig
Start / Stop / Direction15,694 / 16,974 / + [in whole cluster]
15,694 / 16,974 / + [in contig]
Location15694..16974 [in whole cluster]
15694..16974 [in contig]
TypeCDS
Length1,281 bp (426 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase chain length factor subunit
Product (GenBank)chain length factor
GenefrnM
fren-ORF2
Gene (GenBank)frnM
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
  • FrnM; noncatalytic subunit of KAS; ORF2 of Bibb et al
Reference
ACC
PmId
[8181754] Cloning, sequencing and deduced functions of a cluster of Streptomyces genes probably encoding biosynthesis of the polyketide antibiotic frenolicin. (Gene. , 1994)
comment
[PMID:8181754](1994)
frenolicin biosynthesis gene clusterにある6ORFの同定。

ORF2: KSに似たPKS subunitだが、active siteを欠く。
たいてい見られるKSとCLFのoverlapなし。

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上記論文のref論文1993bでfren minimal genesをact minimal genesと組み合わせてpolyketide形成を検討している。

[PMIDなし]Engineered biosynthesis of novel polyketides: manipulation and analysis of an aromatic polyketide synthase with unproven catalytic specificities. J. Am. Chem. Soc., 1993/取り寄せ済

fren-ORF2をact-ORF2の代わりに導入すると、18Cなfrenolicinと16Cなnanaomycinを産生するので、炭素鎖長を調節するようだ。

close this sectionPKS/NRPS Module

CLF21..378

close this sectionSequence

selected fasta
>polyketide synthase chain length factor subunit [chain length factor]
MTTAPSRTAQGAPPGAALPPVFTGIGVAAPNGLGTEEWWAATLRGEHGLRPVTEYDASGH
PGGLVGRVPDFDAARHLPGRLLPQTDRVTRLALVAADEALKDAAVDPARLPEYGASAVTS
NATGGFEFTHREIRKLWTEGPARVSVYESFAWFYAVNTGQISIRHGMRGPGAVVVADQAG
GLDALGQARRVLRKGGVLAVSGGVESALDPWGLAAHASSGTLSRSGDPATAYLPFDRRAL
GTVVGEGGALLTLETPRHAEERDAPRIYGELAGYAATFDPPAGSGRPPGLERAARLALAD
AGLAPGDVDVVFADAAGLPAADAAEAAALRALFGPGGVPVSVPKTQTGRLASGGPALDVA
AALLALRDGLVPPAVHLDEVDPAYGLDLVRDTPRALPLRTALVLARGHGGFNAAVVVRGR
RRPRTA
selected fasta
>polyketide synthase chain length factor subunit [chain length factor]
ATGACCACCGCCCCCTCCCGCACCGCCCAGGGCGCCCCTCCCGGGGCCGCCCTGCCCCCC
GTCTTCACCGGCATCGGCGTCGCCGCCCCCAACGGGCTCGGCACCGAGGAGTGGTGGGCG
GCCACCCTGCGCGGGGAGCACGGTCTGCGGCCCGTCACGGAGTACGACGCGAGCGGCCAC
CCCGGTGGACTCGTCGGCCGCGTCCCGGACTTCGACGCCGCCCGCCACCTGCCCGGCCGC
CTCCTGCCGCAGACCGACCGGGTCACCCGGCTCGCGCTCGTCGCCGCCGACGAGGCACTG
AAGGACGCCGCCGTCGACCCGGCCCGGCTGCCCGAGTACGGGGCGAGCGCCGTCACCTCC
AACGCCACCGGCGGCTTCGAGTTCACCCACCGCGAGATCCGCAAACTCTGGACCGAGGGC
CCGGCCCGCGTCAGCGTCTACGAGTCCTTCGCCTGGTTCTACGCCGTCAACACCGGCCAG
ATCTCCATCCGGCACGGCATGCGCGGCCCCGGCGCCGTCGTCGTGGCCGACCAGGCGGGC
GGCCTCGACGCCCTCGGCCAGGCCCGCCGCGTACTGCGCAAGGGCGGGGTGCTGGCGGTG
AGCGGCGGCGTGGAGTCCGCGCTCGACCCCTGGGGCCTGGCCGCCCACGCCTCGTCGGGC
ACCCTCAGCCGCTCCGGCGACCCGGCCACCGCCTACCTCCCCTTCGACCGGCGTGCCCTC
GGCACCGTCGTCGGCGAGGGCGGCGCCCTCCTCACCCTGGAGACACCCCGGCACGCCGAG
GAGCGCGACGCACCCCGGATCTACGGCGAACTCGCCGGGTACGCCGCCACGTTCGACCCG
CCCGCGGGCTCCGGACGGCCCCCCGGCCTCGAACGCGCCGCGCGCCTCGCCCTCGCCGAC
GCGGGCCTGGCACCCGGGGACGTCGACGTCGTCTTCGCGGACGCGGCGGGGCTCCCCGCC
GCCGACGCCGCCGAGGCCGCCGCCCTGCGCGCGCTCTTCGGCCCCGGCGGCGTTCCGGTG
AGCGTGCCGAAGACCCAGACCGGGCGGCTCGCCTCGGGCGGCCCGGCCCTCGACGTCGCC
GCCGCCCTCCTCGCCCTGCGCGACGGCCTCGTGCCCCCGGCCGTCCACCTCGACGAGGTC
GATCCCGCGTACGGCCTCGACCTCGTCCGCGACACCCCGCGCGCCCTCCCGCTGCGCACG
GCGCTCGTCCTCGCGCGCGGCCACGGCGGCTTCAACGCGGCCGTCGTCGTCCGCGGGCGG
CGGCGGCCCCGTACCGCCTGA
CLF21..378
CLF61..1134

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[21-255] 8.1e-46 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[268-378] 4.6e-25 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[22-273] 7.59999999999998e-46 G3DSA:3.40.47.10 [284-418] 1.9e-38 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[17-264] 1.60000240695997e-52 SSF53901 [227-420] 2.1000026783403e-46 SSF53901
SSF53901   Thiolase-like
SignalP
[1-28] 0.588 Signal
Eukaryota   
[1-28] 0.424 Signal
Bacteria, Gram-positive   
[1-11] 0.539 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Fren_00120 Fren_00120   Fren_00140 Fren_00140
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