Fren_00130 Fren_00130   Fren_00150 Fren_00150

Fren_00140 : CDS information

close this sectionLocation

Organism
StrainAK24158
Entry nameFrenolicin
Contig
Start / Stop / Direction17,047 / 17,298 / + [in whole cluster]
17,047 / 17,298 / + [in contig]
Location17047..17298 [in whole cluster]
17047..17298 [in contig]
TypeCDS
Length252 bp (83 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase acyl carrier protein subunit
Product (GenBank)acyl carrier protein
GenefrnN
fren-ORF3
Gene (GenBank)frnN
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
  • FrnN; ORF3 of Bibb et al
Reference
ACC
PmId
[8181754] Cloning, sequencing and deduced functions of a cluster of Streptomyces genes probably encoding biosynthesis of the polyketide antibiotic frenolicin. (Gene. , 1994)
[8468280] Genetic construction and functional analysis of hybrid polyketide synthases containing heterologous acyl carrier proteins. (J Bacteriol. , 1993)
[7972098] Engineered biosynthesis of novel polyketides: influence of a downstream enzyme on the catalytic specificity of a minimal aromatic polyketide synthase. (Proc Natl Acad Sci U S A. , 1994)
[12705828] Solution structure and backbone dynamics of the holo form of the frenolicin acyl carrier protein. (Biochemistry. , 2003)
comment
[PMID:8181754](1994)
frenolicin biosynthesis gene clusterにある6ORFの同定。

ORF3: PKS acyl carrier protein
配列解析のみ。

--
[PMID: 8468280](1993)
pIJ5214にあるACPをfrenolicin ACPと仮定して、hybrid PKS合成に使っている。
このpIJ5214が[PMID:8181754]で配列解析されている。

--
[PMID:7972098](1994)
minimal PKS, KR, CYCについて、act genesとfren genesを組み合わせた株での産物解析あり。

--
[PMID:12705828](2003) abstract
fren holo-ACPの構造解析。
Related Reference
ACC
Q02054
NITE
Actino_00200
PmId
[16285746] Self-malonylation is an intrinsic property of a chemically synthesized type II polyketide synthase acyl carrier protein. (Biochemistry. , 2005)
[19361520] Probing the Interactions of early polyketide intermediates with the Actinorhodin ACP from S. coelicolor A3(2). (J Mol Biol. , 2009)
[19381365] Preliminary kinetic analysis of acyl carrier protein-ketoacylsynthase interactions in the actinorhodin minimal polyketide synthase. (Mol Biosyst. , 2009)
comment
Blast 5th, id57%, 5e-16
Streptomyces coelicolor_actI-ORF3
[Actino_00200]polyketide synthase acyl carrier protein subunit

--
[PMID: 19361520](2009) abstract
ACP-中間体の相互作用を見てる。

--
[PMID:19381365](2009) abstract
actinorhodin PKSのACPとKSの間の相互作用をkinetic assaysを使用して調査。

--
他にも実験論文多数あり。

Simpson TJ.らはact ACPがself-acylation(malonyl-ACP形成)すると言っている[PMID:9479478](1998), [PMID:9862793](1998), [PMID:16285746](2005)。

これと異なり、Khosla C.らはmalonyl-CoA:ACP transacylase(MCAT)が必要で、一次代謝のFA合成geneを動員すると言っている[PMID:9518007](1998), [PMID:10455191](1999)。

close this sectionPKS/NRPS Module

ACP5..77

close this sectionSequence

selected fasta
>polyketide synthase acyl carrier protein subunit [acyl carrier protein]
MSALTVDDLKKLLAETAGEDDSVDLAGELDTPFVDLGYDSLALLETAAVLQQRYGIALTD
ETVGRLGTPRELLDEVNTTPATA
selected fasta
>polyketide synthase acyl carrier protein subunit [acyl carrier protein]
ATGAGCGCACTGACCGTCGACGACCTCAAGAAACTGCTCGCCGAGACCGCCGGGGAGGAC
GACAGCGTCGACCTCGCCGGAGAACTCGACACGCCCTTCGTGGACCTCGGCTACGACTCC
CTCGCGCTGCTGGAGACGGCCGCCGTGCTCCAGCAGCGGTACGGCATCGCGCTGACTGAC
GAGACGGTCGGCCGGCTGGGCACCCCGCGCGAACTGCTCGACGAGGTCAACACCACCCCG
GCCACCGCCTGA
ACP5..77
ACP13..231

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR009081 Acyl carrier protein-like (Domain)
[2-83] 7.70000171387738e-14 SSF47336
SSF47336   ACP_like
[5-78] 2.1e-17 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[8-76] 7.7e-11 PF00550
PF00550   PP-binding
[5-77] PS50075
PS50075   ACP_DOMAIN
SignalP No significant hit
TMHMM No significant hit
Fren_00130 Fren_00130   Fren_00150 Fren_00150
Page top