Fren_00140 Fren_00140   Fren_00160 Fren_00160

Fren_00150 : CDS information

close this sectionLocation

Organism
StrainAK24158
Entry nameFrenolicin
Contig
Start / Stop / Direction17,862 / 17,380 / - [in whole cluster]
17,862 / 17,380 / - [in contig]
Locationcomplement(17380..17862) [in whole cluster]
complement(17380..17862) [in contig]
TypeCDS
Length483 bp (160 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.4 hypothetical protein
Producthypothetical protein
Product (GenBank)actVI-ORFA homolog
GenefrnO
fren-ORFX
Gene (GenBank)frnO
EC number
Keyword
Note
Note (GenBank)
  • FrnO; ORFX of Bibb et al
Reference
ACC
PmId
[8181754] Cloning, sequencing and deduced functions of a cluster of Streptomyces genes probably encoding biosynthesis of the polyketide antibiotic frenolicin. (Gene. , 1994)
comment
frenolicin biosynthesis gene clusterにある6ORFの同定。

ORFX:
act and otc clusterなどStreptomyces種の芳香族抗生物質生合成gene clusterに見られる
機能不明遺伝子。actVI-ORFA homolog.
Related Reference
ACC
Q53925
NITE
Actino_00020
PmId
[17227452] Possible involvement of ActVI-ORFA in transcriptional regulation of actVI tailoring-step genes for actinorhodin biosynthesis. (FEMS Microbiol Lett. , 2007)
comment
Blast 10th, id42%, 2e-21
Streptomyces coelicolor_ORFA(actVI-ORFA)
Hydroxylacyl-CoA dehydrogenase
[Actino_00020]hypothetical protein

actVI-ORFA mutantはactinorhodin産生減少、中間体(S)-DNPAとshunt産物(S)-NHABを蓄積。相補で回復。
また、同じmutantでactVI-ORF1発現量が減少。相補で回復。
よってActVI-ORFAは、post-PKS tailoring enzymeであるactVI-ORF1の発現を通してactinorhodin産生に影響を与えるregulatory roleを持つ可能性あり。

close this sectionSequence

selected fasta
>hypothetical protein [actVI-ORFA homolog]
MTTTTPPDDVRAGSLPGDAARSAALYTEVQAFYARQAHHLDAVRAEEFAATFAAEGVFAH
SPDTPAARGRAAIAEEVRGFNARRFADDPVQRRHWFSMLDVRPGEDGAVETEFYALVVVT
RPDAALPVIGPSCVVRDVLVREGGELRTLSRQVTQDRTLL
selected fasta
>hypothetical protein [actVI-ORFA homolog]
GTGACGACCACCACCCCGCCGGACGACGTGAGGGCCGGGTCCCTGCCGGGCGACGCGGCG
CGTTCCGCGGCGCTCTACACCGAGGTACAGGCGTTCTACGCGCGCCAGGCACACCACCTG
GACGCGGTACGGGCCGAGGAGTTCGCGGCCACCTTCGCGGCGGAGGGCGTCTTCGCCCAC
TCCCCCGACACCCCGGCGGCCCGGGGCAGGGCGGCGATCGCGGAGGAGGTGCGCGGCTTC
AACGCCCGCCGGTTCGCCGACGACCCCGTCCAGCGGCGGCACTGGTTCTCGATGCTGGAC
GTACGCCCGGGTGAGGACGGCGCCGTCGAGACGGAGTTCTACGCGCTGGTCGTGGTGACC
CGGCCGGACGCGGCGCTCCCGGTGATCGGTCCCAGTTGCGTCGTACGGGACGTCCTGGTG
CGGGAGGGCGGGGAACTGCGCACGCTGTCGCGGCAGGTGACCCAGGACCGCACCTTGCTG
TGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP No significant hit
TMHMM No significant hit
Fren_00140 Fren_00140   Fren_00160 Fren_00160
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