Gelda_00020 Gelda_00020   Gelda_00040 Gelda_00040

Gelda_00030 : CDS information

close this sectionLocation

Organism
Strain17997
Entry nameGeldanamycin
Contig
Start / Stop / Direction19,342 / 20,985 / + [in whole cluster]
9,942 / 11,585 / + [in contig]
Location19342..20985 [in whole cluster]
9942..11585 [in contig]
TypeCDS
Length1,644 bp (547 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.1 modification hydroxylation
Productputative hydroxylase
Product (GenBank)GdmM
Gene
Gene (GenBank)gdmM
EC number
Keyword
Note
Note (GenBank)
  • FAD-dependent monooxygenase; rif19
Reference
ACC
PmId
[18214443] The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997. (Arch Microbiol. , 2008)
comment
geldanamycin生合成clusterにあるpositive regulators GdmRI and GdmRII に関する報告。
cluster schemeあり。

M (gdmM) : Monooxygenase gene

詳細記載なし。
Related Reference
ACC
Q84G20
NITE
Gelda2_00120
PmId
[16085885] Insights into the biosynthesis of the benzoquinone ansamycins geldanamycin and herbimycin, obtained by gene sequencing and disruption. (Appl Environ Microbiol. , 2005)
comment
Blast id88%, 0.0
Streptomyces hygroscopicus(geldanamycininus) NRRL 3602_gdmM
GdmM

herbimycin(hbm)生合成gene clusterを同定し、近しく関連している化合物geldanamycin(gdm) clusterとの比較をしている。

rif19のhomologであるgdmMは、hbm clusterにhomologなし。herbimycinのC-17は酸化されていない。

gdmM不活化mutantは、nonbenzoquinoid geldanamycin analog (KOS-1806)をもたらす。この化合物はherbimycinと同じようにC-17での置換がない。

よってgdmMはC-17での酸化に関与すると提唱されている。
ACC
Q9AE02
NITE
Rifam_00520
PmId
[10430893] Direct evidence that the rifamycin polyketide synthase assembles polyketide chains processively. (Proc Natl Acad Sci U S A. , 1999)
[16079331] Identification of tailoring genes involved in the modification of the polyketide backbone of rifamycin B by Amycolatopsis mediterranei S699. (Microbiology. , 2005)
comment
Blast id50%, 1e-134
Amycolatopsis mediterranei
Putative 3-(3-hydroxylphenyl) propionate hydroxylase
[DoBISCUIT]putative hydroxylase_ORF19

---
[PMID: 10430893](1999)
PKSに関する報告。
rifD, E, Fのmutantにおいて産生されたtetra-decaketideにわたる直鎖状polyketidesからの考察。
tetraketideは非修飾aromatic chromophoreを有し、penta-decaketidesはnaphthoquinoneにoxidative cyclizationあり。よってこれらの修飾はPKSの組み立て中に行われている。

---
[PMID: 16079331](2005)
PKS後修飾に関するgenesの報告。
Orf19: 3-(3-hydroxyphenyl)propionate hydroxylase-like protein

orf19は3-(3-hydroxyphenyl)propionate hydroxylasesに相同性のあるタンパクをコードする。この酵素は、フェノール構造のhydroxylationを触媒するflavoproteinsのグループである。

orf19の破壊はrifamycin Bの産生を止め、tetraketides SY4b and desacetyl-SY4bの蓄積を引き起こした。AA配列alignmentや相補実験もしている。

以上から、rifamycinのnaphthalene環部分の形成はpolyketide鎖延伸の間(RifA:module3とRifB:module4の間)に起こり、Rif-Orf19によってACP-bound tetraketide内にhydroxyl基を導入し、続いて、酵素的または自発的にoxidation, cyclizationされてからRifBの反応へ進む、と提唱している。
ACC
P77397
PmId
[9603882] Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12. (J Bacteriol. , 1998)
comment
Blast id31%, 1e-57
E.coli_mhpA
3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase(EC 1.14.13.127)

Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3-hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI).

close this sectionSequence

selected fasta
>putative hydroxylase [GdmM]
MSGKETAVDVARDTDNGGPKTDVLIVGYGPVGQLLSVLLAQRGRRVTVVERWPEPYRHPR
AVGFDSEAARILASAGIGDSLEKFTEPARDHAWQNTKGETLIDHEVANRGHCTWPEALSA
YQPALESALIEHGATLPRLRVLRGYEAVGLADNGDRVTLTVVGPDGERTDLIASWVVGCD
GANSLVRASVGTTVTDLDFSYDWLICDVRLHEHREFRPNNLEICDPARPRTAVSAGPGHR
RYEFMRVPADDPAHFGTVESAWELLRLFDVTPDNGVLDRHAVYTFQARWAEHWRAGRLLL
AGDSAHLMPPFAGQGMCSGFRDAANLAWKLDLVLSGHAAPALLDTYTSERRAHVRHAVEM
SVGLGRVVCMADPAAAADRDAAMLAARKRNIGPSAARRSVVRPLVDGLLRRDGQGRPAPY
AGQAAPQWRVGRAGGTGLFDDVVGTGFILLYGEDVVPALDARQLTFLDNIGARLVRVVRA
ETPAADLEPGDARDVEGRYLPYLSEMGALAVLVRPDFYVFGIAGDKAELPSLVDDLAEQL
TPTAAPS
selected fasta
>putative hydroxylase [GdmM]
ATGAGCGGGAAGGAGACGGCGGTGGACGTCGCGCGGGACACGGACAACGGCGGGCCCAAG
ACCGATGTGCTGATCGTCGGGTACGGACCGGTGGGGCAGCTGCTGTCGGTGCTGCTGGCC
CAGCGCGGCCGCCGGGTGACGGTGGTGGAGCGCTGGCCGGAGCCGTACCGGCACCCCCGG
GCGGTCGGGTTCGACAGTGAGGCCGCGCGCATCCTGGCCTCGGCCGGGATCGGCGACTCG
CTCGAGAAGTTCACCGAACCCGCGCGGGACCACGCCTGGCAGAACACCAAGGGCGAGACG
CTGATCGACCACGAGGTCGCCAACCGGGGGCACTGCACCTGGCCGGAGGCGCTGTCGGCC
TATCAGCCGGCCCTGGAGTCCGCACTGATCGAACACGGGGCGACGCTGCCGCGGCTGCGG
GTGCTGCGCGGCTACGAGGCGGTGGGACTCGCGGACAACGGTGACCGGGTGACCTTGACC
GTGGTCGGTCCGGACGGCGAGAGGACGGACCTCATCGCTTCGTGGGTGGTCGGCTGCGAC
GGCGCCAACAGCCTGGTGCGGGCGAGTGTCGGCACCACGGTGACGGACCTCGACTTCTCC
TACGACTGGCTGATCTGCGATGTGCGGCTGCATGAGCACCGCGAGTTCCGGCCGAACAAT
CTGGAGATCTGCGATCCGGCCCGCCCGCGGACCGCGGTGTCCGCGGGGCCCGGACACCGG
CGGTACGAGTTCATGCGGGTGCCCGCGGACGACCCCGCGCACTTCGGCACCGTGGAGAGC
GCGTGGGAGCTGCTGCGGCTGTTCGACGTGACGCCCGACAACGGCGTCCTGGACCGGCAT
GCGGTCTACACCTTCCAGGCCCGCTGGGCGGAGCACTGGCGGGCCGGACGGCTGCTGCTG
GCCGGGGACTCGGCGCACCTCATGCCGCCGTTCGCGGGGCAGGGCATGTGCTCCGGATTC
CGTGACGCGGCGAATCTGGCCTGGAAGCTGGACCTGGTGCTGAGCGGGCACGCGGCACCG
GCGCTGCTGGACACCTACACCAGCGAGCGGCGGGCGCATGTGCGGCACGCGGTGGAGATG
TCCGTGGGCCTGGGCCGGGTGGTGTGCATGGCGGACCCGGCCGCGGCGGCGGACCGGGAC
GCGGCGATGCTGGCCGCGCGGAAGCGGAACATCGGGCCGAGCGCCGCGCGCCGCTCGGTG
GTGCGCCCGCTCGTGGACGGGTTGCTGCGGCGGGACGGGCAGGGCCGGCCGGCGCCGTAC
GCCGGACAGGCGGCCCCACAGTGGCGGGTGGGCCGCGCGGGCGGCACCGGGCTGTTCGAC
GATGTGGTGGGCACCGGTTTCATCCTGCTGTACGGCGAGGACGTGGTCCCGGCGCTGGAC
GCGCGGCAGCTGACGTTCCTCGACAACATCGGGGCCCGGCTGGTGCGTGTGGTGCGCGCG
GAAACGCCCGCGGCCGACCTGGAACCAGGGGACGCGCGGGACGTGGAGGGCCGGTATCTG
CCGTATCTGTCGGAGATGGGCGCGCTGGCGGTGCTGGTACGCCCGGACTTCTACGTGTTC
GGCATCGCGGGCGACAAGGCCGAACTGCCCTCGCTGGTGGACGACTTGGCGGAACAACTC
ACCCCGACCGCCGCCCCGTCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002938 Monooxygenase, FAD-binding (Domain)
[20-360] 7.69999999999997e-64 PF01494
PF01494   FAD_binding_3
IPR003042 Aromatic-ring hydroxylase-like (Domain)
[22-44] 1.3000049540733e-34 PR00420 [172-187] 1.3000049540733e-34 PR00420 [295-310] 1.3000049540733e-34 PR00420 [310-326] 1.3000049540733e-34 PR00420 [328-346] 1.3000049540733e-34 PR00420 [346-362] 1.3000049540733e-34 PR00420
PR00420   RNGMNOXGNASE
SignalP No significant hit
TMHMM No significant hit
Gelda_00020 Gelda_00020   Gelda_00040 Gelda_00040
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