Gelda_00100 : CDS information

Location

Organism	Streptomyces hygroscopicus
Strain	17997
Entry name	Geldanamycin
Contig	DQ914285
Start / Stop / Direction	30,746 / 27,963 / - [in whole cluster] 21,346 / 18,563 / - [in contig]
Location	complement(27963..30746) [in whole cluster] complement(18563..21346) [in contig]
Type	CDS
Length	2,784 bp (927 aa)

Annotation

Category	4.1 transcriptional regulator
Product	LuxR family transcriptional activator
Product (GenBank)	GdmRII
Gene
Gene (GenBank)	gdmRII
EC number
Keyword
Note
Note (GenBank)	putative transcriptional regulator; hbmRII
Reference	ACC Q06BL5 PmId [18214443] The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997. (Arch Microbiol. , 2008) comment geldanamycin生合成clusterにあるpositive regulators GdmRI and GdmRII に関する報告。 gdmRI, gdmRII のいずれか一方の欠損株ではgeldanamycinの生産が完全に消失し、欠損した遺伝子をプラスミドで相補すると geldanamycin生産が回復する。 2つの遺伝子の転写は独立に行われ互いを制御していないこと、2つの遺伝子の転写はpolyketide骨格生合成に関与する遺伝子(pks, gdmF, gdnA-O-P)とは協調するが、PKS後修飾遺伝子であるgdmNとは協調していないことも示されている。よって、gdmRI と gdmRII はgeldanamycin生合成経路におけるpolyketide生合成遺伝子に特異的なpositive regulatorsであると結論付けている。
Related Reference	ACC Q84G13 NITE Gelda2_00190 PmId [12586396] Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602. (FEMS Microbiol Lett. , 2003) comment Streptomyces geldanamycininus NRRL 3602でのgeldanamycin生合成clusterにある対応遺伝子。

Sequence

>LuxR family transcriptional activator [GdmRII]
MPFSYAMPVNTERNSHPMDTVLNAAAHLGHAFGDSLVRPGQALLLDGPLACGKTTLLRSF
AERAAAAGRLVITATCSPCERDLPFGVVSQLARGARKSAGGPPEVPGLPDILRGTSDPVD
RAEIARLCHRLCTLLIDYAEHTPLLLAVDDVRHSDPASAHFLLQLVRRLDSARIAAVFTD
DLSLPAPTLPLRYELLRSQRLHRIGLGPLTPDEVADVVVAELGETASPRAGDVYAATGGN
RLLLHTLLADYREHGEARQTGYGQSFLSCLHRNEPIFLDVVRALAVVGFSLPATDLAWLT
GHEPEPIGQVLAALTGAGLMDEGAFRQEAARLSVLNDTPALTRRTLHQRAARLLHDQGRP
ATTIARHLVRAGRIPDSWSPELLLEVAEQVAVGEEASVAVDLLEQSLEQCPHQERRAALQ
AKLAEAEWKINPSTATRHHTPLYGAVRAGRLGLPDSVTLLMQLLWKGGLTEVEGLLAHLR
EDPAATDQLHAIEAALTCTYPWLAERRPAPAHHGGSAATRAAVWPRAGTVLADVLTGGQT
HDTVRRAEEVLRELQLGHDPACQEQAGLFALLALVYGGRIDLASAWCEGALGETGAGPHV
PMRQAVLAAARSEIALRRGDLAEAAERSRAALTHASPGAWGVAVGLPLGSLILACTRMGR
HEEAGFHVAQTVPNAMFKSSYGLHYLYARGHYFLAAGRHQAALADFLLCGELLTDWGLSS
GCDPVPWRIGAAEAWLAQGNHDQARILVYQQLSRPHTDGARARGQSLRLLAATSSAKRRP
QLLNEAVGLFTEQEDKYELARTLWDLSQAYHALGEKKQARRTMRRAWHVAKMCDAASLYE
EWLPADDQSQAVAPAPKADAGIERLTHSERRVASLAAMGYTNREIAGKLYVTASTVEQHL
TRVFRKLDIKHREQLPTELYADRMELA

>LuxR family transcriptional activator [GdmRII]
ATGCCCTTCAGCTATGCCATGCCGGTGAATACCGAGCGGAATTCCCATCCGATGGACACG
GTATTGAATGCCGCGGCGCACCTCGGACACGCGTTCGGTGACTCTTTGGTGCGGCCCGGG
CAGGCTCTTCTCCTGGACGGACCGCTGGCATGCGGTAAGACCACCCTGCTCCGGTCGTTC
GCCGAGCGGGCCGCGGCGGCCGGCCGGCTCGTCATCACGGCGACGTGTTCTCCCTGCGAG
CGGGATCTTCCCTTCGGTGTCGTCTCCCAACTGGCCCGCGGCGCCCGGAAGTCAGCGGGC
GGGCCGCCCGAGGTGCCGGGGCTGCCGGACATCCTCCGCGGGACCAGCGACCCGGTGGAC
CGGGCCGAGATCGCCCGGCTGTGCCATCGGCTGTGCACCTTGCTGATCGACTACGCGGAA
CACACCCCGCTGCTGCTCGCCGTGGACGATGTGCGCCACAGCGATCCGGCCTCCGCGCAC
TTCCTCCTCCAACTGGTGCGGCGGCTGGACTCGGCACGGATCGCCGCCGTGTTCACCGAC
GATCTGAGCCTGCCCGCGCCCACCCTGCCGCTCCGCTACGAACTGCTGCGCTCCCAGCGC
CTGCACCGGATCGGCCTCGGCCCGCTCACCCCCGACGAGGTGGCCGACGTGGTCGTGGCG
GAGCTGGGGGAGACCGCGAGCCCCCGCGCCGGCGATGTCTACGCCGCCACCGGTGGCAAC
CGGCTGCTGCTGCACACCCTGCTGGCCGACTACCGCGAGCACGGCGAGGCCCGCCAGACC
GGCTATGGCCAGTCCTTCCTGAGCTGTCTGCACCGCAATGAGCCGATCTTCCTGGACGTG
GTGCGCGCGCTGGCCGTGGTGGGCTTCTCCTTGCCCGCCACCGACCTGGCCTGGCTGACC
GGACACGAGCCCGAGCCCATCGGCCAGGTGCTGGCGGCGCTGACCGGCGCCGGACTGATG
GACGAGGGCGCGTTCCGGCAGGAGGCGGCGCGGCTGAGCGTGCTCAACGACACGCCGGCG
CTGACCCGCAGGACCCTGCACCAGCGGGCCGCGCGGCTGCTGCACGACCAGGGCAGGCCC
GCCACCACGATCGCCCGTCATCTGGTGCGGGCCGGGCGGATCCCCGATTCATGGTCGCCG
GAGCTGCTGCTGGAAGTGGCCGAGCAGGTCGCGGTGGGCGAAGAGGCGTCCGTCGCCGTC
GACTTACTGGAGCAGTCCCTCGAACAGTGTCCGCACCAGGAGCGGCGCGCCGCCTTGCAG
GCGAAGCTCGCCGAGGCGGAATGGAAGATCAACCCGTCCACCGCCACCCGGCACCACACA
CCGCTGTACGGCGCCGTCCGGGCCGGCCGGCTCGGCCTCCCCGACAGCGTCACCCTGCTC
ATGCAGCTGCTGTGGAAGGGCGGGCTGACCGAGGTGGAGGGGCTGCTCGCCCATCTGCGC
GAGGACCCCGCCGCCACCGACCAGCTCCATGCCATCGAGGCCGCGCTCACCTGCACCTAC
CCCTGGCTCGCGGAGCGGCGGCCGGCCCCGGCGCACCACGGTGGCTCCGCCGCGACGCGG
GCGGCGGTGTGGCCGCGGGCCGGCACCGTACTCGCGGACGTGCTCACCGGCGGGCAGACC
CATGACACCGTCCGGCGGGCCGAGGAGGTGCTGCGCGAACTCCAGCTCGGGCACGACCCG
GCGTGCCAGGAGCAGGCCGGGCTGTTCGCCCTGCTCGCGCTGGTCTACGGCGGCCGGATC
GACCTGGCGTCCGCATGGTGCGAGGGGGCGCTCGGCGAGACCGGCGCGGGGCCGCACGTC
CCGATGCGGCAGGCGGTGCTGGCGGCGGCCAGGTCGGAGATCGCGCTGCGCCGAGGTGAC
CTCGCCGAGGCCGCGGAGCGGTCCCGGGCCGCCCTCACCCACGCCTCTCCCGGCGCCTGG
GGTGTCGCGGTCGGGCTGCCGCTCGGCTCCCTGATCCTGGCGTGTACGCGGATGGGCCGG
CACGAGGAGGCGGGGTTCCACGTCGCGCAGACCGTGCCCAACGCCATGTTCAAAAGCTCC
TACGGGCTGCACTACCTGTACGCGCGCGGCCACTACTTCCTGGCGGCCGGCCGGCACCAG
GCCGCGCTCGCGGACTTTCTGCTGTGCGGCGAGCTGCTCACCGACTGGGGGCTGAGCAGC
GGCTGCGACCCGGTGCCATGGCGGATCGGCGCGGCGGAGGCATGGCTCGCGCAGGGCAAC
CACGACCAGGCCCGGATCCTGGTCTACCAGCAGCTCAGCCGTCCGCATACGGACGGCGCC
CGGGCCCGCGGGCAGTCCTTGCGCCTCCTGGCCGCCACCAGCTCGGCCAAGCGGCGGCCG
CAGCTGCTCAACGAGGCGGTGGGCCTGTTCACCGAGCAGGAGGACAAGTACGAGCTGGCT
CGCACGCTGTGGGACCTCAGCCAGGCGTACCACGCGCTCGGCGAGAAGAAGCAGGCCCGC
CGGACCATGCGCCGGGCCTGGCATGTGGCGAAGATGTGCGACGCCGCGTCGCTGTACGAG
GAGTGGCTGCCGGCCGACGACCAGTCCCAGGCCGTGGCGCCGGCTCCGAAGGCGGACGCC
GGGATCGAGCGGTTGACGCACTCCGAACGGCGTGTCGCGTCGCTGGCCGCCATGGGCTAC
ACCAACCGGGAGATCGCCGGGAAGCTGTATGTCACGGCCAGCACGGTGGAGCAGCATCTG
ACCCGGGTCTTCCGCAAGCTGGACATCAAGCACCGGGAGCAGCTGCCCACCGAACTCTAC
GCGGACCGCATGGAGTTGGCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000792 Transcription regulator LuxR, C-terminal (Domain) PS00622 HTH_LUXR_1 PS50043 HTH_LUXR_2 PF00196 GerE SM00421 helix_turn_helix, Lux Regulon PR00038 HTHLUXR IPR011990 Tetratricopeptide-like helical (Domain) G3DSA:1.25.40.10 no description IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 no description IPR016032 Signal transduction response regulator, C-terminal effector (Domain) SSF46894 C-terminal effector domain of the bipartite response regulators IPR018130 Ribosomal protein S2, conserved site (Conserved_site) PS00962 RIBOSOMAL_S2_1
SignalP	No significant hit
TMHMM	No significant hit

Gelda_00090 Gelda_00110