Gelda_00110 : CDS information

Location

Organism	Streptomyces hygroscopicus
Strain	17997
Entry name	Geldanamycin
Contig	DQ914285
Start / Stop / Direction	32,124 / 31,060 / - [in whole cluster] 22,724 / 21,660 / - [in contig]
Location	complement(31060..32124) [in whole cluster] complement(21660..22724) [in contig]
Type	CDS
Length	1,065 bp (354 aa)

Annotation

Category	2.2 modification addition of starter unit
Product	putative aminodehydroquinate synthase
Product (GenBank)	GdmO
Gene
Gene (GenBank)	gdmO
EC number	4.2.3.-
Keyword	AHBA
Note
Note (GenBank)	putative aminoDHQ synthase; hbmO
Reference	ACC Q06BL4 PmId [18214443] The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997. (Arch Microbiol. , 2008) comment geldanamycin生合成clusterにあるpositive regulators GdmRI and GdmRII に関する報告。 cluster schemeあり。 O (gdmO): Amino DHQ synthase gene 本文での詳細記載なし。
Related Reference	ACC O52549 NITE Rifam_00280 PmId [11278540] Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei S699. (J Biol Chem. , 2001) comment Blast id70%, 1e-119 Amycolatopsis mediterranei_rifG(aroB) 3-dehydroquinate synthase(EC 4.2.3.4) [DoBISCUIT]putative aminodehydroquinate synthase rifamycinと関連ansamycinsのPKS starter 3-amino-5-hydroxybenzoic acid (AHBA)の生合成経路について調査。関連遺伝子の不活化、異種性発現、中間体を基質とした反応確認など。 RifG: Aminodehydroquinate synthase rifH, -G, and -J genesの遺伝子産物は shikimate biosynthesis pathwayに関連する酵素に似ている。rifH, G, Jの各mutantは成長に影響を与えないので、shikimate pathwayに直接関連しないことがわかる。また、mutantでもrifamycin B産生をある程度保つことから、shikimate pathwayのgeneが代替していると考えられる。 AHBA biosynthetic genesをプラスミドに組み込んだS. coelicolor YU105を使ってAHBA生産を調べると、rifGを除いた場合はAHBA biosynthetic genesが全て揃っている場合と比較して、AHBAの生産量が約10%にまで落ちることが示されており、rifGはAHBAの生産に関与していることが示されている。
	ACC P0A4Z4 PmId [17586643] Functional characterization by genetic complementation of aroB-encoded dehydroquinate synthase from Mycobacterium tuberculosis H37Rv and its heterologous expression and purification. (J Bacteriol. , 2007) [1910148] The Mycobacterium tuberculosis shikimate pathway genes: evolutionary relationship between biosynthetic and catabolic 3-dehydroquinases. (Mol Gen Genet. , 1991) comment Blast id45%, 9e-48 Mycobacterium tuberculosis_aroB 3-dehydroquinate synthase(EC 4.2.3.4) [PMID: 17586643] Mycobacterium tuberculosis H37Rv_aroBをクローニング、E. coliで大量発現してhomogeneityに精製、アミノ酸シークエンス。 M. tub_aroBはE. coli aroB deletion mutantを相補することを確認。
	ACC Q84G12 NITE Gelda2_00200 PmId [12586396] Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602. (FEMS Microbiol Lett. , 2003) [16085885] Insights into the biosynthesis of the benzoquinone ansamycins geldanamycin and herbimycin, obtained by gene sequencing and disruption. (Appl Environ Microbiol. , 2005) comment Streptomyces geldanamycininus NRRL 3602でのgeldanamycin生合成clusterにある対応遺伝子。

Sequence

>putative aminodehydroquinate synthase [GdmO]
MTTSTSSPAASSAAPFRQVTVALAERSYTVHIGHGVRRLLPRVVSALGARKAVVVTARPP
EQTPDPKVPSLVVSTRDGEEAKDLAAVTDLCRRFVEFGLTRSDVVVSCGGGTTTDTVGLA
AALYHRGTPVVHVPTSLLAQVDASVGGKTAVNLPEGKNLVGAYWQPAAVLCDLDHLTTLP
EREWRNGLGEIARCHFIGAPDLDGLPLLDQIAASVTLKAGIVAADERDAGLRHILNYGHT
LGHALERATGFALRHGEGVAIGTVFAGRLAGALGRIGPERVAEHHDVVARYGLPTALPAQ
VPVAELVELMRLDKKATDGLTFVLDGPDGPGLVPGIPEDTVSATLAAMPREPAG

>putative aminodehydroquinate synthase [GdmO]
TTGACCACGTCGACCTCCAGTCCGGCCGCCAGTTCCGCCGCTCCTTTCCGGCAGGTCACC
GTCGCGCTGGCCGAGCGCTCGTACACCGTCCACATCGGACACGGTGTGCGGCGGCTGCTC
CCGAGGGTGGTGTCCGCCCTGGGCGCGCGCAAGGCGGTGGTGGTCACCGCGCGGCCCCCG
GAGCAGACGCCCGACCCCAAAGTGCCCTCGCTCGTCGTGTCCACGCGCGACGGGGAGGAG
GCCAAGGACCTGGCCGCCGTGACGGACCTGTGCCGCCGGTTCGTCGAATTCGGGCTGACC
CGCTCGGATGTGGTGGTGTCCTGTGGCGGCGGCACCACGACCGACACGGTGGGCCTGGCC
GCCGCCCTCTACCACCGGGGCACACCGGTCGTCCATGTGCCGACCTCGCTGCTGGCCCAG
GTGGACGCGAGCGTCGGCGGGAAGACGGCGGTGAATCTGCCCGAGGGCAAGAACCTGGTC
GGCGCCTACTGGCAGCCCGCGGCCGTGCTCTGCGACCTCGATCACCTCACCACCCTGCCC
GAACGGGAGTGGCGCAACGGCCTCGGTGAGATCGCCCGCTGCCACTTCATCGGCGCGCCC
GATCTCGACGGGCTGCCGCTGCTCGACCAGATCGCGGCCAGTGTGACGCTCAAGGCGGGC
ATCGTCGCCGCGGACGAACGCGACGCGGGCTTGCGGCACATCCTCAACTACGGACACACA
CTGGGGCATGCGCTGGAACGTGCCACGGGGTTCGCCCTGCGGCACGGGGAGGGCGTGGCC
ATCGGCACGGTCTTCGCCGGCCGGCTCGCCGGTGCGCTGGGCCGTATCGGCCCGGAGCGG
GTGGCCGAGCACCATGATGTGGTCGCCCGTTACGGCCTGCCGACCGCGCTGCCCGCCCAG
GTCCCCGTGGCCGAGCTGGTGGAGCTGATGCGTCTGGACAAGAAGGCCACCGACGGGCTG
ACGTTCGTCCTGGACGGCCCCGACGGGCCCGGACTGGTGCCCGGGATCCCCGAGGACACC
GTCAGCGCCACCCTCGCCGCGATGCCCCGCGAGCCGGCGGGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR016037 3-dehydroquinate synthase AroB (Family) MF_00110 DHQ_synthase
SignalP	Bacteria, Gram-positive Eukaryota Bacteria, Gram-negative
TMHMM	No significant hit

Gelda_00100 Gelda_00115