Gelda_00110 Gelda_00110   Gelda_00120 Gelda_00120

Gelda_00115 : CDS information

close this sectionLocation

Organism
Strain17997
Entry nameGeldanamycin
Contig
Start / Stop / Direction32,394 / 32,200 / - [in whole cluster]
22,994 / 22,800 / - [in contig]
Locationcomplement(32200..32394) [in whole cluster]
complement(22800..22994) [in contig]
TypeCDS
Length195 bp (64 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.5 electron carrier
Productputative ferredoxin
Product (GenBank)
GenegdmFdx
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
Reference
PmId
[21682254] The missing C-17 O-methyltransferase in geldanamycin biosynthesis. (Org Lett. , 2011)
comment
UniProtに該当entryなし。

---
[PMID:21682254](2011)
Table S1.
gdmFdx (64aa) : Ferredoxin

S. hygroscopicus 17997株のgdmFdxはこの仕事でannotateされたとコメントされている。
S. autolyticus CGMCC 0516株のGdmFdxとid100%。
Related Reference
ACC
P18325
PmId
[1846297] Ferredoxins from two sulfonylurea herbicide monooxygenase systems in Streptomyces griseolus. (Biochemistry. , 1991)
comment
Blast id69%, 5e-17
Streptomyces griseolus_subB
Ferredoxin-2(Fd-2)

sulfonylurea herbicideが誘発されたStreptomyces griseolusの可溶性タンパク質フラクションより、Fd-1 と Fd-2の二種類のフェレドキシンを精製し、どちらも、aerobic mixture of NADPH、spinach ferredoxin:NADP oxidoreductase、精製したcytochrome P-450SU1とherbicide基質にsulfonylurea monooxygenase活性を復元できることが示されている。また、cytochromes P-450, P-450SU1 (CYP105A1) と P-450SU2 (CYP105B1)は、それぞれFd-1 と Fd-2の近傍に位置していることが示されている。

close this sectionSequence

selected fasta
>putative ferredoxin
MKVSVDQEKCCAAGHCALAAPEVFDQREEDGAVVLLDPRPPLALRDGVAEASFLCPAAAI
AVED
selected fasta
>putative ferredoxin
GTGAAGGTCTCGGTGGATCAGGAGAAGTGCTGTGCGGCCGGCCACTGCGCGCTGGCGGCC
CCGGAGGTGTTCGACCAGCGGGAGGAGGACGGGGCCGTTGTCCTGCTGGACCCGCGTCCG
CCGCTCGCGCTGCGTGACGGCGTCGCCGAGGCGTCGTTCCTGTGCCCGGCCGCCGCGATC
GCCGTCGAGGACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001080 3Fe-4S ferredoxin (Family)
[5-16] 3.3e-06 PR00352 [16-26] 3.3e-06 PR00352 [48-60] 3.3e-06 PR00352
PR00352   3FE4SFRDOXIN
SignalP No significant hit
TMHMM No significant hit
Gelda_00110 Gelda_00110   Gelda_00120 Gelda_00120
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