Gelda_00115 Gelda_00115   Gelda_00130 Gelda_00130

Gelda_00120 : CDS information

close this sectionLocation

Organism
Strain17997
Entry nameGeldanamycin
Contig
Start / Stop / Direction33,657 / 32,455 / - [in whole cluster]
24,257 / 23,055 / - [in contig]
Locationcomplement(32455..33657) [in whole cluster]
complement(23055..24257) [in contig]
TypeCDS
Length1,203 bp (400 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productcytochrome P450
Product (GenBank)GdmP
Gene
Gene (GenBank)gdmP
EC number
Keyword
  • C-4,5 desaturation
Note
Note (GenBank)
  • P450; PikC subfamily; hbmP
Reference
ACC
PmId
[18214443] The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997. (Arch Microbiol. , 2008)
[21979086] Novel 4,5-dihydro-thiazinogeldanamycin in a gdmP mutant strain of Streptomyces hygroscopicus 17997. (Biosci Biotechnol Biochem. , 2011)
[22849774] Identification of 4,5-dihydro-4-hydroxygeldanamycins as shunt products of geldanamycin biosynthesis. (J Nat Prod. , 2012)
[23656556] 19-[(1'S,4'R)-4'-Hydroxy-1'-methoxy-2'-oxopentyl]geldanamycin, a natural geldanamycin analogue from Streptomyces hygroscopicus 17997. (J Nat Prod. , 2013)
comment
[PMID:18214443](2008)
geldanamycin生合成clusterにあるpositive regulators GdmRI and GdmRII に関する報告。
cluster schemeあり。

P (gdmP): Cytochrome P450 Oxidase gene

本文での詳細記載なし。

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[PMID:21979086](2011)
S.hygroscopicus 17997のgdmP mutantから、4,5-dihydro-geldanamycin と 4,5-dihydro-thiazinogeldanamycin を検出。
後者は中度のanti-HSV-1-virus activityを示し、geldanamycinよりも高い水溶解度を示した。

---
[PMID:22849774](2012)
GdmPはgeldanamycin生合成でdesaturaseとして働くCYP450 oxidaseである（[PMID:18600054] を引用）。

S.hygroscopicus 17997株と、そのgdmP gene disruption mutantの産物を比較。
wild 17997株からのみ、
・(4S)-4,5-dihydro-4-hydroxygeldanamycin
・(4R)-4,5-dihydro-4-hydroxygeldanamycin
を同定した。これら化合物はgeldanamycinより弱い細胞毒性を示す。

また、これら化合物はgdmP mutantでは検出されなかったこと、geldanamycinへの生物変換ができなかったことをふまえ、GdmPによって触媒されるC-4,5 oxidationのshunt産物であるとし、GdmPが触媒するC-4,5 oxidationのschemeを提唱している。

---
[PMID:23656556](2013) abstract
S.hygroscopicus 17997で自然な新規geldanamycin類似体を発見。
gdmP gene disruption mutantで、その4,5-dihydro型も同定された。
これら化合物はgeldanamycinと比べて、水溶解度が上昇し、HepG2 cellsに対する細胞毒性が減った。
Related Reference
ACC
Q84G11
NITE
Gelda2_00210
PmId
[12586396] Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602. (FEMS Microbiol Lett. , 2003)
comment
Streptomyces geldanamycininus NRRL 3602でのgeldanamycin生合成clusterにある対応遺伝子。

close this sectionSequence

selected fasta
>cytochrome P450 [GdmP]
MDRTDEIRGYPELRAAACPFSPPPGYEELRERSAVTRVRMWDGSTPFLVTGYHEARAVLG
DSRFSADGTHKGMPRFTKFEVPAEVFNLGRMDDPEHARIRRMLTAHSTIRRTEAMRPMIQ
GIVDGLLDRLIAEGPPADLVADFAFPLPSQVIAVMLGVSDADFAEFQRASQGVMDFTVST
EEMGAALGVMVDYVSRMCAAKRAAPGDDLLSRLIVDQELTGGLTQQQVVATALVLLLAGH
ETTANMIALSGVLLLRHPEQLARLRADPGLMPNAVDELLRYITIVQEGTGRVAAEDVEVG
GVLIPAGEGVIINLPSANRDPHFADAHELDLGRPNAREHIGFGFGVHQCLGQTLARVELQ
IALETLLRRLPALRLEVPLDDLAFLYESMNFGVARVPVAW
selected fasta
>cytochrome P450 [GdmP]
ATGGACAGGACTGACGAGATACGCGGCTACCCCGAATTGCGGGCTGCTGCATGCCCGTTC
TCACCCCCTCCGGGTTACGAGGAACTGCGTGAGCGGTCCGCCGTCACCCGGGTGCGGATG
TGGGACGGCAGCACCCCCTTTCTCGTCACCGGCTATCACGAGGCGCGGGCCGTGCTCGGC
GACAGTAGGTTCAGCGCCGACGGCACGCACAAGGGGATGCCACGCTTCACCAAGTTCGAG
GTGCCGGCCGAGGTGTTCAACCTCGGGAGGATGGACGATCCGGAGCACGCCCGGATCCGC
CGCATGCTCACCGCGCACTCCACCATCCGGCGCACCGAGGCGATGCGGCCGATGATCCAG
GGCATCGTCGACGGCCTCCTGGACCGGCTGATCGCCGAGGGGCCGCCGGCCGACCTGGTG
GCCGACTTCGCCTTCCCCCTGCCGTCCCAGGTGATCGCCGTGATGCTGGGGGTCTCGGAC
GCCGACTTCGCGGAGTTCCAGCGGGCCTCGCAGGGCGTCATGGACTTCACGGTGTCGACG
GAGGAGATGGGCGCCGCGCTCGGCGTCATGGTCGACTACGTCAGCCGGATGTGTGCGGCC
AAGCGCGCCGCGCCGGGAGACGACCTCCTCAGCCGGCTCATCGTCGACCAGGAGCTGACG
GGCGGGCTGACCCAGCAGCAGGTGGTCGCCACCGCCCTGGTGCTGCTGCTGGCCGGCCAC
GAGACCACGGCCAACATGATCGCCCTGTCCGGCGTCCTGCTGCTGCGCCACCCCGAGCAG
CTCGCCCGGCTGCGGGCGGATCCCGGGCTGATGCCCAACGCGGTGGACGAACTGCTCCGG
TACATCACGATCGTCCAGGAGGGCACCGGACGGGTGGCCGCCGAGGATGTCGAGGTCGGC
GGGGTGCTCATCCCGGCGGGCGAAGGGGTGATCATCAACCTGCCCAGCGCCAACCGCGAT
CCCCACTTCGCGGACGCCCACGAACTGGACCTGGGCCGGCCGAACGCCCGTGAGCACATC
GGGTTCGGCTTCGGTGTGCACCAGTGCCTGGGGCAGACCCTCGCCCGGGTCGAGCTCCAG
ATCGCCCTGGAGACCCTGCTGCGCCGACTTCCGGCGCTGCGCCTGGAGGTCCCGCTCGAC
GACCTGGCGTTTCTGTACGAGTCGATGAACTTCGGCGTCGCCCGTGTGCCCGTCGCCTGG
TGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[207-266] 9.20000000000002e-06 PF00067 [271-370] 9.60000000000003e-21 PF00067
PF00067   p450
[238-255] 1.60000065022528e-06 PR00385 [273-284] 1.60000065022528e-06 PR00385 [340-349] 1.60000065022528e-06 PR00385 [349-360] 1.60000065022528e-06 PR00385
PR00385   P450
[3-400] 1.79999754022378e-92 SSF48264
SSF48264   Cytochrome_P450
[20-400] 4.49999999999996e-100 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
IPR002397 Cytochrome P450, B-class (Family)
[91-102] 4.30000170645869e-49 PR00359 [138-154] 4.30000170645869e-49 PR00359 [155-170] 4.30000170645869e-49 PR00359 [192-214] 4.30000170645869e-49 PR00359 [273-284] 4.30000170645869e-49 PR00359 [291-318] 4.30000170645869e-49 PR00359 [340-349] 4.30000170645869e-49 PR00359 [349-360] 4.30000170645869e-49 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[342-351] PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit
Gelda_00115 Gelda_00115   Gelda_00130 Gelda_00130
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