Gelda_00130 : CDS information

Location

Organism	Streptomyces hygroscopicus
Strain	17997
Entry name	Geldanamycin
Contig	DQ914285
Start / Stop / Direction	36,696 / 33,790 / - [in whole cluster] 27,296 / 24,390 / - [in contig]
Location	complement(33790..36696) [in whole cluster] complement(24390..27296) [in contig]
Type	CDS
Length	2,907 bp (968 aa)

Annotation

Category	4.1 transcriptional regulator
Product	LuxR family transcriptional activator
Product (GenBank)	GdmRI
Gene
Gene (GenBank)	gdmRI
EC number
Keyword
Note
Note (GenBank)	putative transcriptional regulator
Reference	ACC Q06BL2 PmId [18214443] The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997. (Arch Microbiol. , 2008) comment geldanamycin生合成clusterにあるpositive regulators GdmRI and GdmRII に関する報告。 gdmRI, gdmRII のいずれか一方の欠損株ではgeldanamycinの生産が完全に消失し、欠損した遺伝子をプラスミドで相補すると geldanamycin生産が回復する。 2つの遺伝子の転写は独立に行われ互いを制御していないこと、2つの遺伝子の転写はpolyketide骨格生合成に関与する遺伝子(pks, gdmF, gdnA-O-P)とは協調するが、PKS後修飾遺伝子であるgdmNとは協調していないことも示されている。よって、gdmRI と gdmRII はgeldanamycin生合成経路におけるpolyketide生合成遺伝子に特異的なpositive regulatorsであると結論付けている。
Related Reference	ACC Q84G10 NITE Gelda2_00220 PmId [12586396] Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602. (FEMS Microbiol Lett. , 2003) comment Streptomyces geldanamycininus NRRL 3602でのgeldanamycin生合成clusterにある対応遺伝子。

Sequence

>LuxR family transcriptional activator [GdmRI]
MTAEINSSLRRNPPQRGLMSMGLTDYQSRLRDVLGTAARGNGGLLQVVGGPGVGKATLLS
SLEEQAAESDAVCLTASGFAEDTAIPFNIVEQLIRSSAAMGELDVVARWRTAGERYSEAE
HGMLAMLVREISDVLHRIAGGKQLVIAVDDVEHVDYPSLMCLLHIARHASGTRTLVAMSS
GRTHHLCARVQGFHNLYKVEIGTLSESGVVRLLERHADADLADRIGASCHAISGGNPRLV
RALLHDHLMSAPDGSEREVTVGAEFRDSYRWSLLCHPSLLQMAQILAVLGPYGSPRRVAG
LLECGEERAARAIDVLDSAGLLEDGCFRHPAARSVALETLAVEERARLGAKAAELLYADG
ADPIAVAELLVTAGKTPDHKGVAVLWHAAQKNLDEGRTEEAVAGLRLAARADLGRRERLD
IRMALVGALWAGNPAAAEPELDRLMAAIRTESPADIPEQYLCFLLFMVLWFGRFSDVEEA
FTWLSGSGGSGNASSMAALRVTRQWVTFLKPTLIHHFPDQDPSDREVDGLWAANLEHGLQ
LSVDELGMEIGHLQDPRQMADFSPDTMHLLSHSNHFWFAYAYACRIVWALAARGETEAAD
RLCGALFTAADKLNMKTPCAVALSMRAYIQCRQGDFTSAIDLAGTALRSIPPRGWGVAIG
LPLSVLVAAHTAIGRLDEAQRYLRYWVPKEMFDSVVGLEYLRARGQYCLATNRPYAALND
FMVSGMLIDQWPVDFGDLAPWRIDAAEAYLHVHEPVEAKRLALEELKLSPDRPMSTRGRA
LRVLAMAEDPDKRRLLLYQSVKCLREHGDRYGLARTLVELSRDFLSTGETQQARATWHEA
QKLMDECGMSAQHESRRDPVMGGAESPADPQPPGNSEDSGEPGVPEDTALGTSAEDAEPP
VLSEAEWRVATLAASRMTNRQIAKSLYITVSTVEQHLTRVYRKLSVGNRQDLSRRLWPLI
GATGSSSC

>LuxR family transcriptional activator [GdmRI]
ATGACCGCGGAGATCAACTCATCGCTCAGACGCAACCCCCCACAGCGAGGACTGATGAGT
ATGGGACTGACAGATTATCAATCGAGGCTCCGCGACGTTCTCGGCACGGCAGCAAGGGGA
AACGGTGGGCTGCTCCAGGTGGTCGGGGGACCCGGAGTCGGGAAGGCCACCTTATTGAGC
AGTCTGGAGGAGCAGGCGGCCGAGTCGGACGCGGTCTGTCTTACGGCGTCCGGATTCGCG
GAAGACACCGCCATCCCCTTCAATATCGTGGAACAGCTGATCCGATCCTCCGCGGCCATG
GGGGAATTGGACGTCGTGGCGCGCTGGCGGACCGCGGGGGAGCGGTATTCGGAAGCCGAA
CACGGCATGCTGGCCATGCTGGTCCGTGAAATATCGGATGTGCTGCACCGCATCGCGGGC
GGCAAACAACTGGTCATCGCGGTCGATGACGTCGAGCATGTCGACTACCCCTCCCTCATG
TGCCTTCTGCATATCGCCAGACATGCGTCCGGCACCCGTACGCTCGTCGCGATGTCCAGC
GGGCGGACACATCATCTGTGCGCCCGTGTGCAGGGATTCCACAACCTCTACAAGGTGGAG
ATCGGCACACTTTCCGAGTCCGGGGTCGTGCGTCTGCTGGAGCGGCACGCCGACGCCGAC
CTGGCGGACCGGATCGGCGCCTCCTGTCACGCCATCAGCGGTGGCAATCCAAGGCTGGTG
AGGGCGCTGCTGCACGACCATCTCATGTCCGCTCCGGACGGATCGGAAAGAGAGGTCACG
GTCGGAGCGGAGTTCCGGGATTCCTATCGCTGGAGCCTGTTATGCCATCCGTCGCTGCTC
CAGATGGCACAGATCCTCGCCGTACTGGGCCCGTACGGCAGCCCGCGGCGGGTGGCCGGT
CTGCTCGAATGCGGTGAGGAGCGCGCGGCCCGGGCCATCGATGTCCTCGACTCCGCAGGG
CTCCTGGAGGACGGGTGCTTCCGGCACCCCGCGGCCCGATCCGTCGCGCTGGAAACGCTG
GCGGTGGAGGAGCGGGCCCGCCTCGGCGCGAAGGCGGCCGAACTTCTGTATGCCGACGGG
GCCGACCCGATCGCGGTCGCGGAACTCCTGGTCACCGCCGGTAAGACGCCTGATCACAAG
GGTGTGGCGGTGCTCTGGCACGCGGCCCAGAAGAATCTCGACGAGGGCCGTACGGAAGAA
GCGGTCGCCGGTCTGCGGCTGGCCGCTCGCGCCGATCTCGGCCGGCGGGAGCGCCTGGAC
ATTCGCATGGCGCTGGTCGGCGCGTTATGGGCCGGCAATCCGGCGGCCGCCGAACCCGAG
CTCGACCGGCTGATGGCCGCGATACGGACCGAATCCCCCGCGGACATCCCCGAGCAGTAT
CTGTGCTTCCTGCTCTTCATGGTGCTGTGGTTCGGCCGGTTCAGCGATGTCGAAGAAGCC
TTCACATGGCTGTCGGGCAGTGGTGGCTCCGGTAATGCGTCCAGTATGGCGGCGCTGCGG
GTGACCCGGCAGTGGGTCACCTTCCTCAAACCGACGCTGATCCACCACTTCCCCGACCAG
GATCCCTCGGACAGGGAAGTCGACGGGCTGTGGGCGGCCAATCTGGAGCACGGACTCCAG
CTCTCCGTGGACGAACTGGGCATGGAGATCGGGCATCTCCAGGATCCCCGGCAGATGGCG
GACTTCTCCCCCGACACCATGCACCTGTTGTCGCACTCCAACCATTTCTGGTTCGCCTAC
GCGTACGCCTGCCGGATCGTCTGGGCACTGGCCGCGAGGGGAGAGACGGAGGCGGCGGAC
CGGCTGTGCGGGGCGCTGTTCACCGCGGCGGACAAGCTCAATATGAAGACGCCGTGTGCC
GTCGCCCTGTCCATGCGTGCCTATATCCAGTGCCGCCAGGGGGACTTCACCTCGGCCATC
GATCTCGCCGGCACCGCGCTGAGGTCCATCCCGCCACGCGGCTGGGGCGTCGCCATCGGG
TTACCGCTGTCCGTGCTGGTGGCGGCCCACACCGCCATCGGCAGGCTGGACGAGGCCCAG
CGGTATCTGCGTTACTGGGTGCCGAAGGAGATGTTCGACAGCGTTGTCGGATTGGAGTAC
CTCCGGGCCCGGGGGCAATACTGCCTGGCCACCAACCGCCCCTATGCCGCGCTGAACGAT
TTCATGGTGAGCGGGATGCTGATCGACCAGTGGCCCGTGGACTTCGGTGATCTGGCACCC
TGGCGCATCGACGCCGCCGAGGCATATCTGCACGTACATGAACCCGTGGAGGCCAAAAGG
CTCGCCCTGGAAGAGCTCAAGCTGTCGCCCGACCGCCCCATGAGCACCCGCGGCAGAGCG
CTGCGCGTACTGGCCATGGCGGAGGACCCGGACAAACGCAGGCTGCTGCTCTACCAATCC
GTCAAGTGCCTGCGCGAGCACGGCGACCGTTATGGACTCGCCCGCACGCTCGTCGAACTC
AGCCGGGACTTCCTCAGCACCGGGGAAACCCAGCAGGCGCGCGCCACCTGGCATGAGGCG
CAGAAGCTGATGGACGAGTGCGGAATGAGCGCCCAGCACGAGAGCCGACGGGACCCCGTC
ATGGGCGGTGCCGAGAGCCCGGCGGACCCTCAACCGCCGGGAAATTCCGAGGATTCCGGG
GAGCCCGGGGTACCCGAGGATACGGCCCTGGGCACATCGGCCGAAGATGCCGAGCCACCC
GTACTTTCGGAGGCCGAATGGCGCGTGGCCACCCTCGCGGCGTCGAGAATGACCAACCGG
CAGATCGCCAAGAGCCTCTACATCACCGTCAGCACCGTGGAGCAGCATCTGACCCGCGTC
TACCGCAAGCTCTCCGTGGGAAATCGACAGGATCTGTCGCGCCGCCTGTGGCCGCTCATA
GGAGCCACCGGATCGTCCTCCTGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000792 Transcription regulator LuxR, C-terminal (Domain) PF00196 GerE PS50043 HTH_LUXR_2 SM00421 HTH_LUXR IPR011990 Tetratricopeptide-like helical (Domain) G3DSA:1.25.40.10 TPR-like_helical IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR016032 Signal transduction response regulator, C-terminal effector (Domain) SSF46894 Bipartite_resp_reg_C-effector
SignalP	No significant hit
TMHMM	No significant hit

Gelda_00120 last last