Herb_00030 Herb_00030   Herb_00050 Herb_00050

Herb_00040 : CDS information

close this sectionLocation

Organism
StrainAM 3672
Entry nameHerbimycin A
Contig
Start / Stop / Direction5,013 / 3,757 / - [in whole cluster]
5,013 / 3,757 / - [in contig]
Locationcomplement(3757..5013) [in whole cluster]
complement(3757..5013) [in contig]
TypeCDS
Length1,257 bp (418 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative cytochrome P450
Product (GenBank)unknown
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORF4p; ORF4; P450; similar to ORF4_gdm (PikC subfamily)
Reference
ACC
PmId
[16085885] Insights into the biosynthesis of the benzoquinone ansamycins geldanamycin and herbimycin, obtained by gene sequencing and disruption. (Appl Environ Microbiol. , 2005)
comment
herbimycin生合成系gene clusterのクローニングを行い、同じbenzoquinone ansamycin系のgeldanamycin のgene clusterと比較し、一部の遺伝子欠損株を作成してその生合成における関与を調べた文献。

ORF04(1257bp):P450
機能実験、詳細記載無し。
Related Reference
ACC
O87605
NITE
Pikro_00170
PmId
[9778370] Characterization of the macrolide P-450 hydroxylase from Streptomyces venezuelae which converts narbomycin to picromycin. (Biochemistry. , 1998)
[9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998)
[9831532] Hydroxylation of macrolactones YC-17 and narbomycin is mediated by the pikC-encoded cytochrome P450 in Streptomyces venezuelae. (Chem Biol. , 1998)
comment
<Streptomyces venezuelae, BLAST 20; id=41%>

---
[PMID: 9778370](1998)
narbomycinのC-12 hydroxylationを行いpicromycinへと変換するpicK遺伝子をE. coliでC末にHis-tagを付けて発現させて精製し、kinetic解析をしている。

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[PMID: 9770448](1998)
macrolide antibiotic biosynthesis gene cluster 中のpikCについて、挿入変異株を作成し、YC-17 (at C-10 and C-12) と narbomycin (at C-12)の両方のhydroxylationを行っていることを確認している。

---
[PMID: 9831532](1998)
methymycin/pikromycin biosynthetic gene cluster (pik)におけるpikCについて、不活化させると全てのhydroxyl化した macrolideの生産が消滅すること、pikCをHis-tagを付けてE. coliで発現させて精製し、in vitroでYC-17をmethymycin (C-10), neomethymycin (C-12) に、 narbomycinをpikromycin(C-12) にhydroxyl化して変換することを確認している。

close this sectionSequence

selected fasta
>putative cytochrome P450 [unknown]
MTLQESGTAPEALPEPVPLMGCPYKSNPYPLYERMRETGPVHRVRFPSGVLAWLVTGYDA
AHSALGDERLGKNHDRGNDRWRARASIMPEPQHSQLQVHLLHQDPPTHTRMRRFVTDAFT
PRRIEQLRPRFQKLADTLVDALPETGPADLVTGFAARFPFQVLAEVIGLPDELAARFDRD
WGKVVQPVGPADPGRPLYEARLHGLQSYIAEVVAHKREHWADDLLSRLVVAHDRRELSQE
ELDSMIFQLLVAGQEPVTNQITTALIALFRHPAELARLRDNPELMPRAVDELLRYDSAFE
LTTWRFFDQDSDLHGTEVPAGDSVIISLCAANRDPRRFPDPDTLDLDRSPNPHLAFGHGI
HFCPGAALARAELQIALGTLLTRLLGAHLAIDDGDIEWIPAVLGRGTNHLPVGYDRRV
selected fasta
>putative cytochrome P450 [unknown]
ATGACCCTCCAGGAATCCGGGACGGCCCCCGAGGCCCTGCCCGAGCCCGTGCCGCTGATG
GGCTGCCCCTACAAGAGCAACCCGTACCCCCTCTACGAGCGGATGCGCGAGACCGGCCCG
GTGCACCGCGTCCGCTTTCCCAGCGGCGTACTCGCCTGGCTCGTCACCGGCTACGACGCC
GCGCACTCCGCACTGGGCGACGAGCGCCTCGGCAAGAACCACGACCGGGGCAACGACCGC
TGGCGCGCCCGCGCCTCGATCATGCCCGAGCCCCAGCACTCCCAGCTCCAGGTGCACCTC
CTCCACCAGGACCCACCGACCCACACCCGCATGCGGCGCTTCGTCACCGACGCCTTCACC
CCGCGCCGCATCGAGCAACTGAGGCCCCGCTTCCAGAAGCTGGCCGACACCCTCGTGGAC
GCCCTCCCGGAAACCGGCCCCGCGGACCTGGTCACCGGCTTCGCCGCCCGCTTCCCCTTC
CAGGTCCTCGCCGAAGTCATCGGCCTGCCAGATGAGTTGGCGGCCCGCTTCGACCGCGAC
TGGGGCAAGGTCGTCCAGCCGGTCGGACCGGCCGACCCCGGACGCCCGCTGTACGAGGCC
CGTCTGCACGGACTCCAGAGCTACATAGCCGAGGTCGTCGCCCATAAGCGCGAACACTGG
GCGGACGACCTGCTCAGCCGCCTGGTCGTGGCCCATGACCGCCGCGAACTCTCCCAGGAG
GAGCTGGACTCGATGATCTTCCAGCTCCTCGTGGCGGGCCAGGAACCGGTCACCAACCAG
ATCACCACCGCCCTGATCGCCCTCTTCCGCCACCCCGCCGAACTCGCCCGGCTGCGCGAC
AACCCGGAGCTGATGCCCCGCGCGGTCGACGAACTCCTCCGCTACGACAGCGCCTTCGAG
CTGACCACCTGGCGCTTCTTCGACCAGGACAGCGACCTGCACGGCACCGAGGTCCCGGCC
GGCGACTCGGTGATCATCTCCCTGTGCGCCGCCAACCGCGACCCGCGCCGCTTCCCCGAC
CCCGACACCCTCGACCTCGACCGCTCGCCCAACCCCCACCTCGCCTTCGGCCACGGCATC
CACTTCTGCCCCGGCGCGGCCCTGGCCCGCGCCGAACTCCAGATCGCGCTGGGCACCCTC
CTGACCCGCCTCCTCGGAGCGCATCTGGCCATCGACGACGGAGACATCGAGTGGATCCCG
GCCGTCCTCGGCCGGGGGACCAACCACCTGCCCGTCGGCTACGACCGACGCGTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[219-383] 2.3e-21 PF00067
PF00067   p450
[15-417] 6.7999999999999e-104 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
[7-417] 7.19996395544006e-89 SSF48264
SSF48264   Cytochrome_P450
IPR002397 Cytochrome P450, B-class (Family)
[103-114] 2.90000354677981e-44 PR00359 [149-165] 2.90000354677981e-44 PR00359 [207-229] 2.90000354677981e-44 PR00359 [287-298] 2.90000354677981e-44 PR00359 [305-332] 2.90000354677981e-44 PR00359 [333-348] 2.90000354677981e-44 PR00359 [354-363] 2.90000354677981e-44 PR00359 [363-374] 2.90000354677981e-44 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[356-365] PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit
Herb_00030 Herb_00030   Herb_00050 Herb_00050
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