Herb_00110 Herb_00110   Herb_00130 Herb_00130

Herb_00120 : CDS information

close this sectionLocation

Organism
StrainAM 3672
Entry nameHerbimycin A
Contig
Start / Stop / Direction22,946 / 22,155 / - [in whole cluster]
22,946 / 22,155 / - [in contig]
Locationcomplement(22155..22946) [in whole cluster]
complement(22155..22946) [in contig]
TypeCDS
Length792 bp (263 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative hydrolase
Product (GenBank)putative hydrolase
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORF18p; ORF18; similar to Streptomyces coelicolor SC0267
Reference
ACC
PmId
[16085885] Insights into the biosynthesis of the benzoquinone ansamycins geldanamycin and herbimycin, obtained by gene sequencing and disruption. (Appl Environ Microbiol. , 2005)
comment
herbimycin生合成系gene clusterのクローニングを行い、同じbenzoquinone ansamycin系のgeldanamycin のgene clusterと比較し、一部の遺伝子欠損株を作成してその生合成における関与を調べた文献。

ORF18(792bp):hydrolase
機能実験、詳細記載無し。
Related Reference
ACC
Q83WC8
PmId
[12542698] Role of Acinetobacter calcoaceticus 3,4-dihydrocoumarin hydrolase in oxidative stress defence against peroxoacids. (Eur J Biochem. , 2003)
comment
<Acinetobacter calcoaceticus, BLAST 97; id=29%>
Dihydrocoumarin hydrolase(EC 3.1.1.35)_dch

dchをE. coliで発現させて、aromatic lactonesに対するhydrolysis活性とmonochlorodimedonに対するorganic acid-assisted bromination活性の両方を持つことを示している。

close this sectionSequence

selected fasta
>putative hydrolase [putative hydrolase]
MTMRVPVAGGSLDVRVGGDTGPTLVFAHYWGGSADTWNGVLGHLPPGQATVRFDQRGWGT
SRALPGPYHLDQLADDLVRVVEERVSGPFVLVGHSMGGKASQLVAARRPAGLAGLVLVAP
APPQPPAMVTEEYRQGLSHAYDSAETVAHALDHVLTATPLPETVRATAERDSLASGAEAR
QEWPLHGIARDITDATRRIEVPVTVLAAENDMVEPPHILREHLLPHIPHATLTTVPDAGH
LLPAEAPGAVARALGDFLRAIRR
selected fasta
>putative hydrolase [putative hydrolase]
ATGACAATGAGAGTTCCCGTGGCGGGCGGATCGCTCGATGTGCGAGTCGGGGGCGATACC
GGTCCGACGCTGGTGTTCGCCCACTACTGGGGCGGCTCCGCCGACACCTGGAACGGTGTG
CTCGGCCACCTGCCGCCCGGGCAGGCGACGGTCCGCTTCGACCAGCGCGGCTGGGGCACC
TCGCGGGCGCTGCCCGGGCCCTATCACCTCGACCAGCTCGCCGACGATCTCGTCCGTGTG
GTCGAGGAGCGGGTATCGGGGCCGTTCGTCCTCGTCGGCCACTCGATGGGTGGCAAGGCG
AGCCAGCTCGTCGCGGCCCGCCGACCGGCCGGTCTGGCCGGTCTGGTGCTCGTCGCGCCC
GCACCGCCCCAGCCACCCGCCATGGTGACCGAGGAGTACCGACAGGGCCTGTCACACGCC
TACGACTCGGCCGAGACGGTGGCGCACGCCCTCGACCATGTCCTGACCGCCACACCGCTG
CCCGAGACGGTGCGGGCCACCGCGGAGCGCGACAGCCTCGCCTCCGGCGCCGAGGCCCGG
CAGGAGTGGCCCCTGCACGGAATCGCGCGGGACATCACCGATGCCACACGGCGTATCGAG
GTCCCGGTGACGGTGCTTGCCGCGGAGAACGACATGGTGGAGCCGCCGCACATCCTGCGC
GAACACCTCTTGCCCCACATCCCGCACGCGACCCTGACGACCGTCCCTGACGCCGGCCAC
CTGCTCCCCGCGGAAGCCCCCGGCGCCGTAGCCCGAGCCCTCGGAGACTTCCTCAGGGCC
ATTCGGCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP
[1-34] 0.283 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Herb_00110 Herb_00110   Herb_00130 Herb_00130
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