Herb_00130 : CDS information

Location

Organism	Streptomyces hygroscopicus
Strain	AM 3672
Entry name	Herbimycin A
Contig	AY947889
Start / Stop / Direction	23,100 / 23,984 / + [in whole cluster] 23,100 / 23,984 / + [in contig]
Location	23100..23984 [in whole cluster] 23100..23984 [in contig]
Type	CDS
Length	885 bp (294 aa)

Annotation

Category	4.1 transcriptional regulator
Product	putative AraC family transcriptional regulator
Product (GenBank)	putative transcriptional regulator
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	ORF19p; ORF19; AraC family; similar to Streptomyces coelicolor SC0266
Reference	ACC Q49BE5 PmId [16085885] Insights into the biosynthesis of the benzoquinone ansamycins geldanamycin and herbimycin, obtained by gene sequencing and disruption. (Appl Environ Microbiol. , 2005) comment herbimycin生合成系gene clusterのクローニングを行い、同じbenzoquinone ansamycin系のgeldanamycin のgene clusterと比較し、一部の遺伝子欠損株を作成してその生合成における関与を調べた文献。 ORF19(885bp):transcriptional regulator (AraC family) 機能実験、詳細記載無し。
Related Reference	ACC P09377 PmId [8230210] A regulatory cascade in the induction of rhaBAD. (J Mol Biol. , 1993) [10464186] Amino acid-DNA contacts by RhaS: an AraC family transcription activator. (J Bacteriol. , 1999) [10940041] Genetic evidence that transcription activation by RhaS involves specific amino acid contacts with sigma 70. (J Bacteriol. , 2000) comment <Escherichia coli, BLAST 387; id=26%> HTH-type transcriptional activator rhaS --- [PMID: 8230210](1993) RhaSがL-rhamnose catabolic operon, rhaBADの発現に対してdirect L-rhamnose-specific activatorとして機能していることを示した文献。 --- [PMID: 10464186](1999) rhaIと結合することによってrhaBAD の転写を活性化するRhaSについて、rhaIと特異的に結合するアミノ酸残基を同定した文献。 --- [PMID: 10940041](2000) RhaSのsigma 70と相互作用するアミノ酸残基を同定した文献。
Family/Domain	FD IPR018060 comment [IPR018060] DNA binding HTH domain, AraC-type (Domain)

Sequence

>putative AraC family transcriptional regulator [putative transcriptional regulator]
MENGHSAVRQLRYLPAVGSAYGVEVLDFAALRSMDTQRRRTQPQRPDFHVFALVGSGTGS
HEADFHNYRLGEGGAVWIRPGMVHRWSDIDACDGPLILFRPGFLSGFTAAEATTPACWHL
DRQRLPLALLAAEHLGREHSTAVHTPRLASPALLSHLLAALILRALPGTPPSAEAARPGS
RPTEVFRVYRATVEERFAEWHQVADYARALGYDVRTLTRATRAATGTGAKTFLDQRILLE
AKRLLAHTDLPVSGCGRRLGFRDVGNFTTFFRRQTGLPPAAWRAAYSTGGTRGV

>putative AraC family transcriptional regulator [putative transcriptional regulator]
GTGGAAAACGGACACAGTGCGGTGCGGCAACTGCGCTATCTGCCTGCCGTGGGATCGGCG
TACGGGGTGGAGGTCCTGGATTTCGCCGCGCTGCGTTCGATGGACACCCAGCGCCGTCGT
ACCCAGCCGCAGCGCCCCGACTTCCATGTGTTCGCGCTGGTCGGCTCCGGAACCGGCAGC
CATGAAGCGGACTTCCACAACTACCGGCTGGGGGAAGGCGGCGCCGTGTGGATCCGGCCG
GGCATGGTGCACCGCTGGAGCGATATCGACGCCTGCGACGGCCCGCTGATCCTGTTCCGG
CCCGGTTTCCTTTCCGGCTTCACGGCGGCAGAGGCCACCACACCGGCGTGCTGGCATCTG
GACCGGCAGCGGCTGCCCCTCGCCCTGCTCGCGGCCGAACATCTCGGCCGCGAGCACAGC
ACGGCAGTGCACACACCACGCCTGGCATCCCCCGCCCTGCTCTCCCACCTGCTGGCGGCG
CTGATCCTGCGCGCACTCCCGGGCACACCGCCCTCGGCCGAGGCGGCAAGACCCGGCAGC
CGGCCAACCGAAGTGTTCCGGGTCTATCGGGCCACCGTCGAAGAGCGCTTCGCCGAATGG
CACCAGGTGGCCGACTACGCACGGGCGTTGGGCTACGACGTACGCACCCTCACCCGGGCA
ACGCGCGCCGCCACCGGCACGGGCGCCAAGACCTTTCTCGACCAGCGCATCCTGCTGGAG
GCGAAACGGCTGCTCGCCCACACCGATCTGCCGGTCAGCGGCTGCGGCCGACGCCTCGGC
TTCCGGGACGTCGGCAACTTCACCACGTTCTTCCGGCGCCAGACCGGCCTGCCCCCCGCC
GCGTGGCGCGCCGCGTACAGCACCGGCGGCACACGCGGCGTCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003313 Transcription regulator HTH, AraC, arabinose-binding/dimerisation (Domain) PF02311 AraC_binding IPR009057 Homeodomain-like (Domain) SSF46689 Homeodomain_like G3DSA:1.10.10.60 Homeodomain-rel IPR011051 RmlC-like cupin domain (Domain) SSF51182 RmlC_like_cupin IPR018060 DNA binding HTH domain, AraC-type (Domain) PS01124 HTH_ARAC_FAMILY_2 SM00342 HTH_ARAC
SignalP	Eukaryota
TMHMM	No significant hit

Herb_00120 Herb_00140