Herb_00270 Herb_00270   Herb_00290 Herb_00290

Herb_00280 : CDS information

close this sectionLocation

Organism
StrainAM 3672
Entry nameHerbimycin A
Contig
Start / Stop / Direction81,528 / 81,334 / - [in whole cluster]
81,528 / 81,334 / - [in contig]
Locationcomplement(81334..81528) [in whole cluster]
complement(81334..81528) [in contig]
TypeCDS
Length195 bp (64 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.5 electron carrier
Productputative ferredoxin
Product (GenBank)HbmFdx
Gene
Gene (GenBank)hbmFdx
EC number
Keyword
Note
Note (GenBank)
  • ferredoxin
Reference
ACC
PmId
[16085885] Insights into the biosynthesis of the benzoquinone ansamycins geldanamycin and herbimycin, obtained by gene sequencing and disruption. (Appl Environ Microbiol. , 2005)
comment
herbimycin生合成系gene clusterのクローニングを行い、同じbenzoquinone ansamycin系のgeldanamycin のgene clusterと比較し、一部の遺伝子欠損株を作成してその生合成における関与を調べた文献。

HbmFdx(195bp):ferredoxin
hbmFdx自体の機能実験は無し。
Related Reference
ACC
P18325
PmId
[1846297] Ferredoxins from two sulfonylurea herbicide monooxygenase systems in Streptomyces griseolus. (Biochemistry. , 1991)
comment
<Streptomyces griseolus, BLAST 2; id=69%>
Ferredoxin-2(Fd-2)_subB

sulfonylurea herbicideが誘発されたStreptomyces griseolusの可溶性タンパク質フラクションより、Fd-1 と Fd-2の二種類のフェレドキシンを精製し、どちらも、aerobic mixture of NADPH、spinach ferredoxin:NADP oxidoreductase、精製したcytochrome P-450SU1とherbicide基質にsulfonylurea monooxygenase活性を復元できることが示されている。また、cytochromes P-450, P-450SU1 (CYP105A1) と P-450SU2 (CYP105B1)は、それぞれFd-1 と Fd-2の近傍に位置していることが示されている。

close this sectionSequence

selected fasta
>putative ferredoxin [HbmFdx]
MKVSVDQDKCCAAGHCALAAPEVFDQREEDGTVVLLDARPPLELRDSVAEASFLCPAAAI
AVED
selected fasta
>putative ferredoxin [HbmFdx]
GTGAAGGTCTCGGTGGATCAGGACAAGTGCTGCGCGGCCGGGCATTGCGCGCTGGCGGCG
CCGGAGGTTTTCGACCAGCGGGAGGAGGACGGGACCGTGGTCCTGCTGGATGCGCGGCCG
CCGCTCGAGCTGCGCGACAGCGTCGCCGAGGCGTCGTTCCTCTGCCCGGCCGCCGCGATC
GCGGTCGAGGACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001080 3Fe-4S ferredoxin (Family)
[5-16] 1.60000065022528e-06 PR00352 [16-26] 1.60000065022528e-06 PR00352 [48-60] 1.60000065022528e-06 PR00352
PR00352   3FE4SFRDOXIN
SignalP No significant hit
TMHMM No significant hit
Herb_00270 Herb_00270   Herb_00290 Herb_00290
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