Herb_00260 Herb_00260   Herb_00280 Herb_00280

Herb_00270 : CDS information

close this sectionLocation

Organism
StrainAM 3672
Entry nameHerbimycin A
Contig
Start / Stop / Direction81,257 / 80,193 / - [in whole cluster]
81,257 / 80,193 / - [in contig]
Locationcomplement(80193..81257) [in whole cluster]
complement(80193..81257) [in contig]
TypeCDS
Length1,065 bp (354 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.2 modification addition of starter unit
Productputative aminodehydroquinate synthase
Product (GenBank)HbmO
Gene
Gene (GenBank)hbmO
EC number4.2.3.-
Keyword
  • AHBA
Note
Note (GenBank)
  • putative aminoDHQ synthase
Reference
ACC
PmId
[16085885] Insights into the biosynthesis of the benzoquinone ansamycins geldanamycin and herbimycin, obtained by gene sequencing and disruption. (Appl Environ Microbiol. , 2005)
comment
herbimycin生合成系gene clusterのクローニングを行い、同じbenzoquinone ansamycin系のgeldanamycin のgene clusterと比較し、一部の遺伝子欠損株を作成してその生合成における関与を調べた文献。

HbmO(1,065bp):amino DHQ synthase
hbmO自体の機能実験は無し。
Related Reference
ACC
O52549
NITE
Rifam_00280
PmId
[11278540] Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei S699. (J Biol Chem. , 2001)
comment
21st, id70%, 1e-119
Amycolatopsis mediterranei_rifG
3-dehydroquinate synthase(EC 4.2.3.4)_rifG(aroB)
[DoBISCUIT]putative aminodehydroquinate synthase

rifamycinと関連ansamycinsのPKS starter 3-amino-5-hydroxybenzoic acid (AHBA)の生合成経路について調査。関連遺伝子の不活化、異種性発現、中間体を基質とした反応確認など。

RifG: Aminodehydroquinate synthase

rifH, -G, and -J genesの遺伝子産物は shikimate biosynthesis pathwayに関連する酵素に似ている。rifH, G, Jの各mutantは成長に影響を与えないので、shikimate pathwayに直接関連しないことがわかる。また、mutantでもrifamycin B産生をある程度保つことから、shikimate pathwayのgeneが代替していると考えられる。

AHBA biosynthetic genesをプラスミドに組み込んだS. coelicolor YU105を使ってAHBA生産を調べると、AHBA biosynthetic genesが全て揃っている場合と比較して、rifGを除いた場合はAHBAの生産量が約10%にまで落ちることが示されており、rifGはAHBAの生産に関与していることが示されている。
ACC
P0A4Z4
PmId
[17586643] Functional characterization by genetic complementation of aroB-encoded dehydroquinate synthase from Mycobacterium tuberculosis H37Rv and its heterologous expression and purification. (J Bacteriol. , 2007)
[1910148] The Mycobacterium tuberculosis shikimate pathway genes: evolutionary relationship between biosynthetic and catabolic 3-dehydroquinases. (Mol Gen Genet. , 1991)
comment
100th, id45%, 1e-49
Mycobacterium tuberculosis_aroB
3-dehydroquinate synthase(EC 4.2.3.4)

[PMID: 17586643]
Mycobacterium tuberculosis H37Rv_aroBをクローニング、E. coliで大量発現してhomogeneityに精製、アミノ酸シークエンス。
M. tub_aroBはE. coli aroB deletion mutantを相補することを確認。

close this sectionSequence

selected fasta
>putative aminodehydroquinate synthase [HbmO]
MTTSTSTSATGSASPARQVTVGLAERSYTVHIGHGVQRLLPQVVAALGARRAVVVTARPA
GQTPDPGVPSLVVPARDGEEAKDLAAVTDLCRRFVGFGLTRSDVVVSCGGGTTTDTVGLA
AALYHRGTPVVHVPTSLLAQVDASVGGKTAVNLPEGKNLVGAYWQPAAVLCDLEHLKTLP
DREWRNGLGEIARCHFIGAPDLDGLPLLDQISASVTLKAGIVAADERDSGLRHLLNYGHT
LGHALERATGFALRHGEGVAIGTVFAGRLAGALGRIGPERVAEHHDVVSRYGLPTGLPPD
VSVSELVELMRLDKKATDGLTFVLDGPEGPGLVRGIPEDTVSTTLAAMPRAPAW
selected fasta
>putative aminodehydroquinate synthase [HbmO]
TTGACCACGTCGACTTCCACTTCGGCCACCGGCTCCGCGTCCCCTGCCCGGCAGGTCACC
GTCGGGCTGGCCGAACGCTCCTACACCGTTCACATCGGCCACGGTGTGCAGCGGCTGCTG
CCACAGGTGGTGGCCGCCCTGGGCGCGCGCAGGGCGGTGGTGGTCACCGCGCGGCCCGCC
GGGCAGACCCCCGACCCGGGAGTGCCCTCGCTCGTCGTGCCCGCCCGCGACGGGGAGGAG
GCCAAGGACCTGGCCGCCGTGACGGACCTGTGCCGCCGGTTCGTCGGATTCGGACTCACC
CGCTCGGATGTGGTGGTGTCCTGTGGCGGCGGCACCACGACCGACACGGTGGGCCTGGCC
GCCGCCCTCTACCACCGGGGCACCCCGGTCGTTCATGTGCCGACCTCGCTGCTCGCCCAG
GTGGACGCGAGCGTCGGCGGGAAGACCGCGGTGAATCTGCCCGAGGGCAAGAACCTGGTC
GGCGCCTACTGGCAGCCCGCGGCCGTGCTCTGCGACCTCGAGCATCTGAAAACCCTGCCC
GACCGGGAATGGCGCAACGGCCTCGGTGAGATCGCCCGCTGCCACTTCATCGGCGCGCCC
GACCTCGACGGGCTGCCGCTGCTCGACCAGATCTCGGCCAGTGTGACGCTCAAGGCGGGC
ATCGTCGCGGCGGACGAACGCGACTCCGGCCTGCGCCACCTCCTCAACTACGGACACACC
TTGGGGCATGCGCTGGAACGCGCCACCGGGTTCGCACTGCGCCACGGCGAGGGCGTGGCC
ATCGGCACGGTCTTCGCCGGCCGGCTCGCCGGCGCGCTGGGGCGCATCGGCCCGGAACGG
GTCGCCGAGCACCATGACGTCGTATCCCGCTACGGCCTGCCGACCGGGCTGCCGCCGGAT
GTCTCGGTGTCCGAGCTGGTGGAGCTGATGCGCCTGGACAAGAAGGCCACCGACGGGCTG
ACGTTCGTCCTGGACGGCCCCGAGGGGCCCGGACTGGTGCGCGGGATCCCCGAGGACACC
GTCAGCACCACCCTCGCGGCGATGCCCCGCGCGCCCGCATGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR016037 3-dehydroquinate synthase AroB (Family)
[17-353] 2.89997807423186e-78 PIRSF001455
PIRSF001455   DHQ_synth
SignalP
[1-24] 0.371 Signal
Bacteria, Gram-positive   
[1-18] 0.5 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Herb_00260 Herb_00260   Herb_00280 Herb_00280
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