Herb_00260 : CDS information

Location

Organism	Streptomyces hygroscopicus
Strain	AM 3672
Entry name	Herbimycin A
Contig	AY947889
Start / Stop / Direction	79,917 / 77,137 / - [in whole cluster] 79,917 / 77,137 / - [in contig]
Location	complement(77137..79917) [in whole cluster] complement(77137..79917) [in contig]
Type	CDS
Length	2,781 bp (926 aa)

Annotation

Category	4.1 transcriptional regulator
Product	putative LuxR family transcriptional regulator
Product (GenBank)	HbmRII
Gene
Gene (GenBank)	hbmRII
EC number
Keyword
Note
Note (GenBank)	putative transcriptional regulator; LuxR-type
Reference	ACC Q49BD2 PmId [16085885] Insights into the biosynthesis of the benzoquinone ansamycins geldanamycin and herbimycin, obtained by gene sequencing and disruption. (Appl Environ Microbiol. , 2005) comment herbimycin生合成系gene clusterのクローニングを行い、同じbenzoquinone ansamycin系のgeldanamycin のgene clusterと比較し、一部の遺伝子欠損株を作成してその生合成における関与を調べた文献。 HbmRII(2,781bp):LuxR-type transcriptional regulator hbmRII自体の機能実験は無し。
Related Reference	ACC Q06BL5 NITE Gelda_00100 PmId [18214443] The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997. (Arch Microbiol. , 2008) comment <gdmRII, Streptomyces hygroscopicus, BLAST 3; id=91%> gdmRI, gdmRIIのいずれか一方の欠損株ではgeldanamycinの生産が完全に消失し、欠損した遺伝子をプラスミドで相補すると geldanamycin生産が回復すること、二つの遺伝子の転写は独立に行われ互いを制御していないこと、二つの遺伝子の転写はpolyketide生合成に関与する遺伝子と協調し、post-PKS modification 遺伝子である gdmNとは協調していないことが示されており、gdmRI と gdmRIIはgeldanamycin 生合成経路におけるpolyketide 生合成遺伝子特異的なpositive regulatorsであると結論付けている。

Sequence

>putative LuxR family transcriptional regulator [HbmRII]
MPYSYAMPVNTERNSRPLGGVLNTAVHLGHPFGDSLVRPGEALLVDGPLACGKTTLLRSF
AERASESGYLTITATCSPSERDLPFGVVSQLARGAWKSPGGPPEVPGLPDILRGAGDPVD
RAGIARLCHRLCTSLIDHAEHTPLLLAVDDVRHSDPASAAFLLQLVRRLDSARIAAVFTD
DLSLSSSLPLRYELLRAQHLRRIGLGPLSPGQVAEVVVAELGETARRHVGDILAATGGNR
LLLHTLLSDYREYGEARQTGYGQSFLSCLHRNEPIFLDVVRALAVVGSALPAADLAWLTG
HEPEPISQVLAALTGAGLMDEGAFRQETARLSVLNDMPAQARRTLHQRAARLLHDQGRPA
ATIARHLVRAGQIPDSWSAEVLLEVAEQVAVGEEASIAVDLLEQSFEQCPHEERRAALRA
KLAEAEWKINPSTATRHHAPLYGAVRAGRLGLPDSVTLLMQLLWKGGLSEVEGLLAHLRA
DPAATDQLHAIEAALTCTYPWLAERRAAPPHHGGAAATRAAAWPRAGTVLADVLTGGQTH
DTVRRAEEVLRELQLGHDPACHEQAGLFALLALVYGGRNDLASAWCEGALGETGGGPHVP
MRQAVLSAARSEIALRRGDLAEAAEQSRAALTHASPGAWGVAIGLPLGALILACTRMGRH
EEAGFHVAQTVPNAMFKSSYGLHYLYARGHYFLAAGRHQAALADFLLCGELLTDWGLSSG
CDPVPWRIGAAEAWLAQGNHDQARILVYQQLSRPHTDGARARGQSLRLLAATSSAKRRPQ
LLNEAVGLFTEQEDKYELARTLWDLSQAYHALGEKKQARRTMRRAWHVAKMCDAASLYEE
WLPADDQGHATGPTPKSDTAIERLTHSERRVASLAAMGYTNREIAGKLYVTASTVEQHLT
RVFRKLDIKHREQLPTELYADRMELA

>putative LuxR family transcriptional regulator [HbmRII]
ATGCCCTACAGCTATGCCATGCCGGTGAATACCGAGCGGAATTCCCGTCCGCTGGGCGGG
GTATTGAATACCGCGGTGCACCTCGGACATCCATTCGGTGACTCTTTGGTGCGGCCCGGG
GAGGCTCTTCTCGTGGACGGACCGCTGGCATGCGGGAAGACGACCCTGCTCCGGTCGTTC
GCCGAACGAGCCTCGGAATCCGGCTATCTCACCATCACCGCGACATGCTCCCCCAGCGAG
CGGGACCTTCCCTTCGGCGTCGTCTCGCAATTGGCCCGCGGCGCCTGGAAGTCACCGGGT
GGGCCGCCCGAGGTACCGGGGCTGCCGGACATCCTCCGCGGGGCCGGCGATCCGGTGGAC
CGGGCCGGGATCGCCCGGCTGTGCCACCGGCTGTGCACCTCGCTGATCGACCACGCGGAA
CACACACCACTGCTCCTCGCCGTGGACGATGTCCGGCACAGCGACCCGGCCTCCGCAGCC
TTCCTCCTGCAACTGGTACGGCGCCTGGACTCGGCGCGCATCGCGGCCGTATTCACCGAC
GACCTGAGCCTGTCCTCGTCCCTGCCGCTCCGCTACGAACTGCTGCGCGCCCAGCACCTG
CGCCGCATCGGCCTGGGCCCCCTTTCCCCGGGTCAGGTGGCCGAAGTGGTCGTGGCGGAG
CTGGGGGAGACCGCGCGCCGCCACGTCGGCGACATCCTCGCCGCCACCGGTGGCAACCGG
CTGCTGCTGCACACCTTGCTCTCCGACTACCGTGAGTACGGCGAGGCCCGCCAGACCGGC
TACGGCCAGTCCTTCCTGAGCTGTCTGCACCGCAATGAGCCGATCTTCCTGGACGTGGTG
CGCGCGCTGGCCGTGGTGGGCTCCGCCCTGCCCGCCGCCGACCTCGCCTGGCTGACCGGA
CACGAGCCCGAGCCCATCAGCCAGGTGCTCGCGGCGCTGACCGGGGCCGGGCTGATGGAC
GAGGGCGCGTTCCGGCAGGAGACGGCCCGGCTGAGCGTGCTCAACGACATGCCCGCGCAG
GCCCGCAGGACCCTGCACCAGCGGGCCGCGCGGCTGCTGCACGACCAGGGCAGGCCCGCC
GCCACGATCGCCCGTCATCTGGTGCGGGCCGGGCAGATCCCCGACTCGTGGTCGGCCGAG
GTGCTTCTGGAAGTGGCCGAGCAGGTGGCGGTGGGCGAAGAGGCGTCCATCGCCGTCGAC
TTACTGGAGCAGTCCTTCGAGCAATGCCCGCACGAAGAGCGCCGCGCCGCCCTGCGGGCG
AAGCTCGCCGAGGCGGAGTGGAAGATCAATCCGTCCACCGCCACGCGGCACCACGCACCG
CTGTACGGCGCCGTCCGGGCCGGCCGGCTCGGCCTCCCCGACAGCGTCACCCTGCTCATG
CAGCTCCTGTGGAAGGGGGGACTGAGCGAGGTGGAGGGGCTGCTCGCCCATCTGCGCGCG
GACCCCGCGGCCACCGACCAGCTCCATGCCATCGAGGCGGCACTCACCTGCACCTATCCC
TGGCTGGCCGAGCGGCGGGCGGCACCGCCGCACCACGGTGGCGCCGCGGCGACACGGGCG
GCGGCGTGGCCCCGGGCCGGTACGGTCCTGGCCGACGTGCTCACCGGCGGGCAGACCCAT
GACACCGTCCGCCGGGCCGAGGAGGTGCTGCGCGAACTTCAGCTCGGACACGACCCGGCC
TGCCATGAGCAGGCGGGGCTGTTCGCCCTGCTCGCCCTGGTCTACGGCGGCCGGAACGAC
CTGGCGTCCGCCTGGTGCGAGGGTGCGCTCGGCGAGACCGGTGGTGGACCGCACGTCCCG
ATGCGGCAGGCGGTGCTGTCGGCCGCCAGGTCGGAGATCGCACTGCGCCGCGGTGACCTA
GCCGAGGCCGCGGAGCAGTCCCGCGCCGCCCTCACCCACGCCTCCCCCGGCGCCTGGGGC
GTCGCCATCGGACTGCCGCTCGGCGCCCTCATCCTGGCGTGTACGCGGATGGGGCGGCAC
GAGGAGGCGGGGTTCCATGTCGCGCAGACCGTGCCCAACGCCATGTTCAAAAGCTCCTAC
GGGCTGCACTACCTGTACGCGCGCGGCCACTACTTCCTGGCGGCGGGCCGCCACCAGGCC
GCGCTCGCGGACTTCCTGCTCTGCGGCGAACTGCTCACCGACTGGGGGCTGAGCAGCGGC
TGCGACCCGGTGCCGTGGCGGATCGGGGCGGCGGAGGCATGGCTGGCGCAGGGCAACCAC
GACCAGGCCCGGATCCTGGTGTACCAGCAGCTCAGCCGTCCGCACACGGACGGCGCCCGG
GCCCGCGGACAGTCGTTACGCCTTCTGGCGGCCACCAGTTCGGCCAAGCGGCGGCCGCAG
TTGCTCAACGAGGCGGTGGGCCTGTTCACCGAGCAGGAGGACAAGTACGAGCTGGCCCGC
ACCCTGTGGGACCTCAGCCAGGCGTACCACGCGCTCGGCGAGAAGAAGCAGGCCCGCCGG
ACCATGCGCCGGGCCTGGCATGTGGCGAAGATGTGCGACGCGGCGTCGCTGTACGAGGAG
TGGCTGCCGGCCGACGACCAGGGCCATGCCACCGGGCCAACGCCGAAGTCGGACACCGCG
ATCGAGCGGCTGACACACTCCGAACGGCGGGTCGCGTCGCTGGCCGCCATGGGCTATACC
AACCGGGAGATCGCCGGGAAGCTGTACGTCACGGCCAGCACGGTGGAGCAGCATCTGACC
CGGGTGTTCCGCAAGCTGGACATCAAGCACCGGGAGCAACTGCCGACCGAACTGTACGCC
GACCGGATGGAGTTGGCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000792 Transcription regulator LuxR, C-terminal (Domain) PR00038 HTHLUXR PS00622 HTH_LUXR_1 PS50043 HTH_LUXR_2 PF00196 GerE SM00421 HTH_LUXR IPR011990 Tetratricopeptide-like helical (Domain) G3DSA:1.25.40.10 TPR-like_helical IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR016032 Signal transduction response regulator, C-terminal effector (Domain) SSF46894 Bipartite_resp_reg_C-effector
SignalP	No significant hit
TMHMM	No significant hit

Herb_00250 Herb_00270