Herb_00250 : CDS information

Location

Organism	Streptomyces hygroscopicus
Strain	AM 3672
Entry name	Herbimycin A
Contig	AY947889
Start / Stop / Direction	76,381 / 77,037 / + [in whole cluster] 76,381 / 77,037 / + [in contig]
Location	76381..77037 [in whole cluster] 76381..77037 [in contig]
Type	CDS
Length	657 bp (218 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative O-methyltransferase
Product (GenBank)	HbmG
Gene
Gene (GenBank)	hbmG
EC number	2.1.1.-
Keyword	methoxymalonyl-ACP
Note
Note (GenBank)	OMT; similar to FkbG (putative O-methyltransferase in methoxymalonyl-ACP biosynthesis)
Reference	ACC Q49BD3 PmId [16085885] Insights into the biosynthesis of the benzoquinone ansamycins geldanamycin and herbimycin, obtained by gene sequencing and disruption. (Appl Environ Microbiol. , 2005) comment herbimycin生合成系gene clusterのクローニングを行い、同じbenzoquinone ansamycin系のgeldanamycin のgene clusterと比較し、一部の遺伝子欠損株を作成してその生合成における関与を調べた文献。 HbmG(657bp):O-methyltransferase in methoxymalonyl-ACP biosynthesis hbmG自体の機能実験は無し。
Related Reference	ACC Q8KUG5 NITE Ansam_00190 PmId [11996558] Functional expression of genes involved in the biosynthesis of the novel polyketide chain extension unit, methoxymalonyl-acyl carrier protein, and engineered biosynthesis of 2-desmethyl-2-methoxy-6-deoxyerythronolide B. (J Am Chem Soc. , 2002) comment 83rd(Q8KUG5) 57%, 1e-45 Actinosynnema pretiosum subsp. auranticum_asm17 O-methyltransferase S. lividansで、AT domainsのひとつがmethoxymalonateに特異的なATで置換されているerythromycin PKSのカセットとasm13-17を同時発現すると、予測された位置にmethoxymalonateが取り込まれた6-deoxyerythronolide Bの派生体が産生された。よって、asm13-17はmethoxymalonyl-ACP生合成に十分である。また、methyltransferaseをコードするasm17を削除すると、そのhydroxy類似体ではなく6-deoxyerythronolide Bが得られたので、伸長unitのmethylationはその取り込みのために必要である。
	ACC P28034 PmId [1894629] Molecular cloning, induction and taxonomic distribution of caffeoyl-CoA 3-O-methyltransferase, an enzyme involved in disease resistance. (J Biol Chem. , 1991) [9037150] Structure of the parsley caffeoyl-CoA O-methyltransferase gene, harbouring a novel elicitor responsive cis-acting element. (Plant Mol Biol. , 1997) comment 196th(P28034) 38%, 3e-31 Petroselinum crispum (Parsley)(パセリ) Caffeoyl-CoA O-methyltransferase(EC 2.1.1.104) caffeoyl-CoA-specific 3-O-methyltransferaseの活性測定あり。
	ACC Q9KIE7 NITE Asco_00070 PmId [15179529] Engineered biosynthesis of 16-membered macrolides that require methoxymalonyl-ACP precursors in Streptomyces fradiae. (Appl Microbiol Biotechnol. , 2004) comment Blast 12th, id60%, 3e-69 Streptomyces hygroscopicus subsp. ascomyceticus_fkbG FkbG S.hygroscopicus由来fkbG-Kを導入することにより、S. fradiaeはmalonyl-CoA, methylmalonyl-CoA, ethylmalonyl-CoAに加えてmethoxymalonyl-ACPもPKS前駆体として利用できるようになることを、midecamycin PKS genes（最後から2番目の縮合ステップでmethoxymalonateを要求）の異種性発現産物で確認している。

Sequence

>putative O-methyltransferase [HbmG]
MAQQVDVTEKILGYVRELSLRDDEILAGLRAETAGLPAAQAMQVMPEEGQLLGLLVRLVG
ARSVLEIGTFTGYSTLCMARALPTDGTLVTCDITAKWPGLGRPFWERAEVADRIDVRIGD
AKETLAGLRREGREFDLVFIDADKTGYAHYYEESLAMLRRGGLIVLDNTLFFGRVTDPAA
QDADTAALREVNKLLREDGRVEISMLTVGDGITLAVKR

>putative O-methyltransferase [HbmG]
ATGGCTCAGCAAGTCGATGTGACCGAAAAAATTCTCGGATATGTCCGGGAACTGTCCCTG
CGCGATGACGAGATCCTGGCCGGGCTGCGGGCGGAGACCGCGGGTCTGCCCGCCGCCCAG
GCCATGCAGGTGATGCCCGAGGAGGGCCAGCTCCTCGGGCTGCTGGTACGGCTCGTCGGC
GCCCGTTCGGTGTTGGAGATCGGCACCTTCACCGGTTACAGCACGCTGTGCATGGCGCGG
GCCCTGCCGACCGACGGCACGCTGGTGACCTGCGACATCACGGCGAAGTGGCCGGGGCTC
GGCCGCCCGTTCTGGGAGCGCGCCGAGGTGGCGGACCGCATCGACGTGCGCATCGGTGAC
GCCAAGGAGACGCTCGCCGGGCTGCGGCGGGAGGGCCGGGAGTTCGACCTGGTCTTCATC
GACGCGGACAAGACCGGATACGCGCACTATTACGAGGAGTCGCTGGCGATGCTCCGGCGC
GGCGGGCTCATCGTCCTGGACAACACCCTCTTCTTCGGCCGGGTGACCGACCCCGCCGCG
CAGGACGCCGACACCGCCGCCCTGCGCGAGGTGAACAAGCTGCTCCGGGAGGACGGACGC
GTCGAGATCAGCATGCTCACCGTTGGTGATGGCATCACGCTCGCGGTCAAACGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002935 O-methyltransferase, family 3 (Family) PF01596 Methyltransf_3
SignalP	No significant hit
TMHMM	No significant hit

Herb_00240 Herb_00260