Herb_00240 : CDS information

Location

Organism	Streptomyces hygroscopicus
Strain	AM 3672
Entry name	Herbimycin A
Contig	AY947889
Start / Stop / Direction	76,137 / 75,271 / - [in whole cluster] 76,137 / 75,271 / - [in contig]
Location	complement(75271..76137) [in whole cluster] complement(75271..76137) [in contig]
Type	CDS
Length	867 bp (288 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative oxidoreductase
Product (GenBank)	HbmK
Gene
Gene (GenBank)	hbmK
EC number
Keyword	methoxymalonyl-ACP
Note
Note (GenBank)	similar to FkbK (methoxymalonyl-ACP biosynthesis pathway protein)
Reference	ACC Q49BD4 PmId [16085885] Insights into the biosynthesis of the benzoquinone ansamycins geldanamycin and herbimycin, obtained by gene sequencing and disruption. (Appl Environ Microbiol. , 2005) comment herbimycin生合成系gene clusterのクローニングを行い、同じbenzoquinone ansamycin系のgeldanamycin のgene clusterと比較し、一部の遺伝子欠損株を作成してその生合成における関与を調べた文献。 HbmK(867bp):methoxymalonyl-ACP biosynthesis pathway hbmK自体の機能実験は無し。
Related Reference	ACC Q8KUG1 NITE Ansam_00150 PmId [11996558] Functional expression of genes involved in the biosynthesis of the novel polyketide chain extension unit, methoxymalonyl-acyl carrier protein, and engineered biosynthesis of 2-desmethyl-2-methoxy-6-deoxyerythronolide B. (J Am Chem Soc. , 2002) comment 14th(Q8KUG1) 62%, 3e-80 Actinosynnema pretiosum subsp. auranticum_asm13 3-hydroxyacyl-CoA dehydrogenase S. lividansで、AT domainsのひとつがmethoxymalonateに特異的なATで置換されているerythromycin PKSのカセットとasm13-17を同時発現すると、予測された位置にmethoxymalonateが取り込まれた6- deoxyerythronolide Bの派生体が産生された。よって、asm13-17はmethoxymalonyl-ACP生合成に十分である。
	ACC Q7BQ74 PmId [16983083] Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units. (Proc Natl Acad Sci U S A. , 2006) comment 132nd(Q7BQ74) 37%, 1e-45 Bacillus cereus_zmaG ZmaG ZmaGは、珍しいPKS伸長units, hydroxymalonyl-ACP and aminomalonyl-ACPの生合成に共通して関連する。 ZmaE or ZmaIと共に働く。 ZmaI: eryl-ZmaH(ACP)→aminomalonyl-ZmaH(ACP) ZmaE: glyceryl-ZmaD(ACP)→hydroxymalonyl-ZmaD(ACP)
	ACC P52041 PmId [8655474] Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. (J Bacteriol. , 1996) comment 306th(P52041) 36%, 1e-40 Clostridium acetobutylicum_hbd 3-hydroxybutyryl-CoA dehydrogenase beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase (BHBD)の遺伝子のクローニングを行い、E. coliで過剰発現させたところBHBDの細胞外への分泌を確認。また、C. acetobutylicumで発現させ、BHBD activityの上昇を確認している。
	ACC Q9ADL9 NITE Soraph_00040 PmId [12039053] Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. (Gene. , 2002) [15289572] Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. (Microbiology. , 2004) comment Blast 323rd, id41%, 3e-40 Sorangium cellulosum_sorD Beta-hydroxybutyryl-CoA dehydrogenase --- [PMID: 12039053](2002) Soraphen A biosynthetic gene clusterの報告。 SorD(295aa): beta-Hydroxybutyryl-CoA dehydrogenase Module 3,7で取り込まれるMethoxymalonyl-CoAを合成する。本ORFはCH2OHをCHOにする。 --- [PMID: 15289572](2004) sorC破壊株はsoraphen A非産生。 S. lividansでのsor genes異種性発現。 SorABRCDFE株 + cinnamate SorABRCDFE株 + badA(benzoate-CoA ligase) + benzoate or cinnamate の組み合わせでSoraphen A産生。 SorABRCDFE株で発現されているのは、the sorA, and the sorB4M, sorRCDFE operons.
	ACC Q9KIE3 NITE Asco_00110 PmId [15179529] Engineered biosynthesis of 16-membered macrolides that require methoxymalonyl-ACP precursors in Streptomyces fradiae. (Appl Microbiol Biotechnol. , 2004) comment Blast 12th, id62%, 5e-82 Streptomyces hygroscopicus subsp. ascomyceticus_fkbK FkbK S.hygroscopicus由来fkbG-Kを導入することにより、S. fradiaeはmalonyl-CoA, methylmalonyl-CoA, ethylmalonyl-CoAに加えてmethoxymalonyl-ACPもPKS前駆体として利用できるようになることを、midecamycin PKS genes（最後から2番目の縮合ステップでmethoxymalonateを要求）の異種性発現産物で確認している。

Sequence

>putative oxidoreductase [HbmK]
MSGDAEAPRLTVLGAGTMGLGITSLAVGHGIPVTVVEVDEAKAGRTRAAVTERLRMAQLM
GALPAGRPRGELTVTASLADGRNATAVVEAVTEDTPTKAKVLEAVAGLTGARVPLISNTS
SIPIDELAGHIADPARLVGTHFMNPPYLIPTVEVIRGPRTGEAVMTAVTDLLRALERKPV
VVGDGPGFVTSRVLHPMINDAIRVVQEGTATVEAVDVLMRDCLGHRTGPLRTADLIGLDN
LADSLRVLHERTGDSRCAPSELLLKKVRDGHLGRKSGRGFYAYGKELA

>putative oxidoreductase [HbmK]
ATGAGCGGAGATGCGGAAGCGCCGCGGCTCACGGTGCTGGGCGCAGGAACGATGGGACTG
GGAATCACCTCTCTCGCCGTCGGGCACGGAATCCCGGTGACGGTCGTCGAGGTGGACGAG
GCCAAGGCCGGCCGAACACGGGCCGCGGTCACCGAACGGCTCCGCATGGCGCAGCTCATG
GGAGCCCTGCCGGCCGGCCGCCCGCGGGGCGAGCTGACGGTCACCGCGTCCCTGGCCGAC
GGCCGGAACGCCACCGCGGTGGTGGAGGCCGTCACCGAGGACACCCCCACCAAGGCCAAG
GTGCTCGAAGCGGTCGCCGGGCTCACCGGAGCGCGGGTGCCGCTGATCTCGAACACCTCC
TCCATCCCGATCGACGAACTGGCCGGTCATATCGCCGATCCGGCACGGCTGGTCGGCACC
CACTTCATGAACCCGCCCTATCTGATCCCCACGGTGGAGGTGATCCGCGGTCCCCGGACC
GGGGAAGCGGTGATGACCGCCGTGACGGATCTGCTGCGTGCGCTGGAGCGCAAGCCGGTC
GTCGTCGGCGACGGGCCGGGGTTCGTCACCAGCCGGGTGCTGCACCCGATGATCAACGAC
GCGATCCGGGTGGTGCAGGAGGGCACGGCCACGGTGGAGGCCGTGGACGTACTCATGCGG
GACTGCCTGGGCCACCGCACCGGGCCGCTGCGCACCGCGGACCTGATCGGCCTGGACAAC
CTGGCCGACTCGCTGCGCGTGCTGCACGAGCGCACCGGCGACTCCCGGTGCGCGCCGAGC
GAACTGCTGCTGAAGAAGGTCCGCGACGGCCACCTGGGCCGCAAGTCGGGCCGCGGGTTC
TACGCATACGGGAAGGAACTGGCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal (Domain) PF00725 3HCDH IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding (Domain) PF02737 3HCDH_N IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain) SSF48179 6DGDH_C_like IPR013328 Dehydrogenase, multihelical (Domain) G3DSA:1.10.1040.10 Opine_DH IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd IPR022694 3-hydroxyacyl-CoA dehydrogenase (Family) PIRSF000105 HCDH
SignalP	Bacteria, Gram-negative Eukaryota
TMHMM	No significant hit

Herb_00230 Herb_00250