Grana_00220 Grana_00220   Grana_00240 Grana_00240

Grana_00230 : CDS information

close this sectionLocation

Organism
StrainTü22
Entry nameGranaticin
Contig
Start / Stop / Direction27,124 / 26,093 / - [in whole cluster]
27,124 / 26,093 / - [in contig]
Locationcomplement(26093..27124) [in whole cluster]
complement(26093..27124) [in contig]
TypeCDS
Length1,032 bp (343 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative oxidoreductase
Product (GenBank)putative FMN-dependent monooxygenase
Gene
Gene (GenBank)gra-orf29
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998)
comment
entire granaticin gene cluster(gra cluster)の報告。
ORF29: 配列解析でsugar ORFsに割り当てられているが？付き。
Related Reference
ACC
Q8TXY4
PmId
[1953299] Purification and properties of N5,N10-methylenetetrahydromethanopterin reductase (coenzyme F420-dependent) from the extreme thermophile Methanopyrus kandleri. (Arch Microbiol. , 1991)
comment
223rd(Q8TXY4) 27%, 2e-13
Methanopyrus kandleri_mer
5,10-methylenetetrahydromethanopterin reductase(EC 1.5.99.11)

いろいろな条件下(温度,pH,添加物)で活性を見ている。
電子供与体としてF420(メタン発酵に用いられる特別な補酵素)に特異的。
Family/Domain
FD
IPR011251
comment
[IPR011251] Luciferase-like domain (Domain)
function; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

close this sectionSequence

selected fasta
>putative oxidoreductase [putative FMN-dependent monooxygenase]
MRLAVNLVHQGAGELARAAERSGYAAALAPEGYRSDAASVLGLVAGVTERIALISGVMQI
PGRPPAMTALTAATLDSLSGGRFRLGLGVSNPDVSVGWYGVPFARPLERTREYVDIVRRA
LEGDPVTYQGTHFEVGGPAPLHLLTERRPGRLPVLLAAVGPRNLRLAGEIADGWLGVFTS
PEAVAESVAEIRKGRGTDDLDGFEVLPSLPTAVADSAQDAADMLRGQYVYLMGIGDPDRN
FYCAMARSMGYEAEVAAFLERLAAGDRTGAGAALPLGFIDRTALIGPVDRIADRMRAYAD
AGVTTLGVMVSAAATGLEGRLAIVEAAARALERSGTATTAGTG
selected fasta
>putative oxidoreductase [putative FMN-dependent monooxygenase]
GTGCGGCTCGCCGTGAACCTCGTCCACCAGGGTGCCGGCGAACTCGCCCGCGCCGCCGAG
CGGTCGGGCTACGCCGCCGCCCTCGCCCCGGAGGGATACCGGTCCGACGCGGCCAGTGTC
CTCGGGCTGGTGGCAGGGGTCACCGAACGCATCGCGCTGATCTCGGGTGTGATGCAGATC
CCGGGCCGCCCGCCGGCCATGACCGCGCTGACCGCCGCCACGCTCGACTCGCTGAGCGGC
GGCCGCTTCCGGCTCGGGCTCGGGGTGTCCAACCCCGACGTGTCGGTGGGCTGGTACGGC
GTGCCGTTCGCCCGGCCGCTGGAGCGCACCAGGGAGTACGTCGACATCGTCCGGCGCGCC
CTGGAGGGCGACCCTGTCACCTACCAGGGCACTCACTTCGAGGTCGGCGGGCCCGCCCCG
CTGCATCTGCTGACCGAGCGGCGGCCCGGCCGGCTGCCGGTGCTGCTCGCGGCGGTCGGT
CCGCGCAACCTGCGGCTCGCGGGGGAGATCGCGGACGGCTGGCTCGGCGTCTTCACCTCC
CCCGAAGCCGTGGCCGAGTCCGTCGCGGAGATCCGCAAGGGCCGGGGCACCGACGACCTG
GACGGCTTCGAGGTGCTGCCCAGCCTGCCCACCGCCGTCGCCGACAGCGCGCAGGATGCG
GCGGACATGCTGCGCGGCCAGTACGTGTACCTGATGGGCATCGGCGATCCCGACCGCAAC
TTCTACTGCGCGATGGCCCGTTCGATGGGCTACGAGGCCGAGGTCGCCGCCTTCCTGGAG
CGGCTCGCCGCCGGTGACCGCACGGGCGCAGGGGCCGCACTGCCCCTCGGTTTCATCGAC
CGTACGGCGCTGATCGGCCCGGTCGACCGGATCGCCGACCGGATGCGCGCCTACGCCGAC
GCGGGCGTGACCACCCTCGGGGTCATGGTCTCGGCCGCCGCGACCGGCCTGGAAGGGCGA
CTGGCGATCGTCGAAGCGGCGGCCCGCGCACTGGAGCGCTCCGGCACCGCCACCACCGCC
GGCACCGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR011251 Luciferase-like domain (Domain)
[1-331] 1.90000694315261e-62 SSF51679
SSF51679   Luciferase_like
[13-304] 1.69999999999998e-67 PF00296
PF00296   Bac_luciferase
[13-329] 2.99999999999998e-66 G3DSA:3.20.20.30
G3DSA:3.20.20.30   Luciferase_like
SignalP No significant hit
TMHMM No significant hit
Grana_00220 Grana_00220   Grana_00240 Grana_00240
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