Grana_00280 : CDS information

Location

Organism	Streptomyces violaceoruber
Strain	Tü22
Entry name	Granaticin
Contig	AJ011500
Start / Stop / Direction	31,629 / 31,886 / + [in whole cluster] 31,629 / 31,886 / + [in contig]
Location	31629..31886 [in whole cluster] 31629..31886 [in contig]
Type	CDS
Length	258 bp (85 aa)

Annotation

Category	1.1 PKS
Product	polyketide synthase acyl carrier protein subunit
Product (GenBank)	acyl carrier protein
Gene
Gene (GenBank)	gra-orf3
EC number
Keyword	type II PKS
Note
Note (GenBank)
Reference	ACC P12885 PmId [2583128] Structure and deduced function of the granaticin-producing polyketide synthase gene cluster of Streptomyces violaceoruber Tu22. (EMBO J. , 1989) [9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998) [8468280] Genetic construction and functional analysis of hybrid polyketide synthases containing heterologous acyl carrier proteins. (J Bacteriol. , 1993) [9305965] Utilization of enzymatically phosphopantetheinylated acyl carrier proteins and acetyl-acyl carrier proteins by the actinorhodin polyketide synthase. (Biochemistry. , 1997) comment [PMID: 2583128](1989) granaticin生合成gene clusterの一部6.5 kb regionの配列解析報告。 -- [PMID: 9831526](1998) entire granaticin gene cluster(gra cluster)の報告。 ORF3: Acyl carrier protein (ACP)[PKS] 配列解析。actI-3に似ている。 gra-ORF1-5はact clusterと同じ並び。 --- [PMID: 8468280](1993) actinorhodin clusterのACPをgranaticin ACPで置換しても機能的なPKSが発現され、actVII mutantで見られるshunt産物である鎖状polyketide mutactinを産生する。 --- [PMID: 9305965](1997) abstract E.coliのholo-ACP synthaseを使用して形成されたgranaticin holo-ACPは、actinorhodin PKSのKS-CLFと共に機能的であり、正規のactinorhodin PKSによって形成されるものと同じ芳香族polyketidesを合成する。
Related Reference	ACC Q02054 NITE Actino_00200 PmId [16285746] Self-malonylation is an intrinsic property of a chemically synthesized type II polyketide synthase acyl carrier protein. (Biochemistry. , 2005) [19361520] Probing the Interactions of early polyketide intermediates with the Actinorhodin ACP from S. coelicolor A3(2). (J Mol Biol. , 2009) [19381365] Preliminary kinetic analysis of acyl carrier protein-ketoacylsynthase interactions in the actinorhodin minimal polyketide synthase. (Mol Biosyst. , 2009) comment Blast 5th, id62%, 3e-18 Streptomyces coelicolor_SCO5089(actI-ORF3) Actinorhodin polyketide synthase acyl carrier protein -- [PMID: 19361520](2009) abstract ACP-中間体の相互作用を見てる。 -- [PMID:19381365](2009) abstract actinorhodin PKSのACPとKSの間の相互作用をkinetic assaysを使用して調査。 -- 他にも実験論文多数あり。 Simpson TJ.らはact ACPがself-acylation(malonyl-ACP形成)すると言っている[PMID:9479478](1998), [PMID:9862793](1998), [PMID:16285746](2005)。これと異なり、Khosla C.らはmalonyl-CoA:ACP transacylase(MCAT)が必要で、一次代謝のFA合成geneを動員すると言っている[PMID:9518007](1998), [PMID:10455191](1999)。

PKS/NRPS Module

ACP	5..78

Sequence

>polyketide synthase acyl carrier protein subunit [acyl carrier protein]
MARLTLDGLRTILVACAGEDDGVDLSGDILDITFEELGYDSLALMESASRIERELGVALA
DGDINEELTPRVLLDLVNGAQAEAA

>polyketide synthase acyl carrier protein subunit [acyl carrier protein]
ATGGCTCGGCTCACCCTCGACGGACTGCGCACCATCCTCGTCGCCTGCGCCGGAGAGGAC
GACGGCGTCGACCTCTCCGGCGACATCCTCGACATCACGTTCGAGGAGCTCGGCTACGAC
TCCCTCGCCCTCATGGAGAGCGCCTCCCGCATCGAGCGCGAGCTCGGAGTCGCCCTCGCC
GACGGTGACATCAACGAGGAGCTGACCCCCCGCGTCCTCCTCGACCTCGTCAACGGCGCC
CAGGCCGAGGCGGCCTGA

ACP	5..78

ACP	13..234

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006162 Phosphopantetheine attachment site (PTM) PS00012 PHOSPHOPANTETHEINE IPR009081 Acyl carrier protein-like (Domain) PS50075 ACP_DOMAIN SSF47336 ACP_like G3DSA:1.10.1200.10 ACP_like PF00550 PP-binding
SignalP	No significant hit
TMHMM	No significant hit

Grana_00270 Grana_00290