Griseo_00020 : CDS information

Location

Organism	Streptomyces sp.
Strain	JP95
Entry name	Griseorhodin A
Contig	AF509565
Start / Stop / Direction	2,296 / 3,117 / + [in whole cluster] 2,296 / 3,117 / + [in contig]
Location	2296..3117 [in whole cluster] 2296..3117 [in contig]
Type	CDS
Length	822 bp (273 aa)

Annotation

Category	4.1 transcriptional regulator
Product	putative transcriptional regulator
Product (GenBank)	putative transcriptional activator GrhR2
Gene
Gene (GenBank)	grhR2
EC number
Keyword	SARP family
Note
Note (GenBank)
Reference	ACC Q8KSZ0 PmId [12323376] A gene cluster from a marine Streptomyces encoding the biosynthesis of the aromatic spiroketal polyketide griseorhodin A. (Chem Biol. , 2002) comment griseorhodin A生合成gene clusterのクローニング、シークエンス、異種性発現。 grhR2: Transcriptional activator 配列解析のみ。 GrhR1 and GrhR2はSARP familyのtranscriptional activatorsに似ている。 SARPsは下流遺伝子の転写を活性化するためpromoter領域に結合し、しばしばregulationに関連したTTA codonを含むがgrhR1 or grhR2には存在しない。
Related Reference	ACC P25047 NITE Adria_00210 PmId [1729206] Regulation of secondary metabolism in Streptomyces spp. and overproduction of daunorubicin in Streptomyces peucetius. (J Bacteriol. , 1992) [7868593] Functional characterization and transcriptional analysis of the dnrR1 locus, which controls daunorubicin biosynthesis in Streptomyces peucetius. (J Bacteriol. , 1995) [8971703] Purification and characterization of the DNA-binding protein DnrI, a transcriptional factor of daunorubicin biosynthesis in Streptomyces peucetius. (Mol Microbiol. , 1996) [16545946] Feedback regulation of doxorubicin biosynthesis in Streptomyces peucetius. (Res Microbiol. , 2006) [20529134] Modulation of dnrN expression by intracellular levels of DnrO and daunorubicin in Streptomyces peucetius. (FEMS Microbiol Lett. , 2010) [20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010) comment Blast 15th, id54%, 1e-69 Streptomyces peucetius_dnrI Regulatory protein dnrI -- [PMID: 1729206](1992) ATCC 29050 strainでdnrI を不活化したら、RHO産生とdaunorubicin (DNR)産生ができなくなり、DNRへの抵抗性が著しく減った。 --- 同じ著者らの続報。 [PMID: 7868593](1995) dnrI::aphII mutantでは、DNR生合成におけるearly- and late-acting enzymesの両方をコードする転写物が存在しない。よってdnrI は、直接または間接的にDNR生合成遺伝子のすべてを調節している。 dnrI::aphII and dnrN::aphII strainsでresistance genes=drrAB and drrC転写物は消失。よって、dnrI はDNR抵抗性の重大なregulatorであり、dnrNはdnrI を通して間接的にその影響を発現する、と示唆される。 -- [PMID: 8971703](1996) abstract DNA-binding assays. ・dnrG-dpsABCD と dpsEF の間・dnrC gene と dnrDKPSQ の間の遺伝子間領域を含んだDNA断片へ特異的に結合する。 -- [PMID: 16545946](2006) abstract dnrI promoter活性を増やすためにはDnrO and DnrN の両方が必要であった。 -- [PMID: 20529134]や[PMID: 20158521]で、dnrO, dnrN, dnrI, DNRが関与する細胞内DNR濃度維持システムが提唱されている。 dnrI promoterにはDnrNが結合し活性化される。

Sequence

>putative transcriptional regulator [putative transcriptional activator GrhR2]
MRIRVLGPLNADVNGVSVVPSAGKPRQVLSLLALYPGQIMPVPTLMEEIWGAAPPVSALT
TLQTYILQLRRRLGTAMGPEAPHTAKDVLATRHGGYVLQIPADSTDVHAYERKVREGQAA
FEAGDDESAADTLREALALWQGPALVDVRCGPALSIEVLRLEESRLVTVERRIDADLRLG
RHCEISAELRDLTARHPHHEGLHSQAMVALYRSGRQAAALDLYRKLRVRLIEELGVEPSP
QMQRMHQAMLSVDPALDLATGPRRGSTFDLYAA

>putative transcriptional regulator [putative transcriptional activator GrhR2]
ATGAGGATCCGGGTTCTGGGACCGCTGAATGCCGACGTCAATGGTGTCTCCGTCGTTCCC
TCGGCGGGCAAACCACGCCAAGTCCTTTCCCTGCTCGCGCTGTACCCGGGGCAGATCATG
CCCGTGCCGACGCTGATGGAGGAGATCTGGGGCGCCGCCCCGCCCGTCAGCGCGCTGACC
ACGCTCCAGACCTACATACTCCAGCTGCGGCGCCGGCTCGGCACCGCCATGGGGCCCGAG
GCCCCGCACACCGCGAAGGACGTCCTCGCCACGCGGCACGGCGGGTACGTGCTCCAGATC
CCGGCGGACAGCACGGACGTGCACGCCTACGAGCGCAAGGTGCGGGAGGGGCAGGCGGCC
TTCGAGGCCGGTGACGACGAGAGCGCTGCCGACACGCTGCGGGAGGCGCTGGCGCTCTGG
CAGGGACCCGCGCTGGTCGACGTGCGCTGCGGCCCGGCGCTGAGCATCGAGGTGCTCCGG
CTGGAGGAGAGCAGGCTGGTGACGGTGGAGCGGCGCATCGACGCCGATCTGCGGCTGGGC
CGTCACTGCGAGATCAGCGCCGAACTGCGGGACCTGACGGCGCGCCACCCGCACCACGAG
GGGCTGCACTCGCAGGCCATGGTGGCCCTGTACCGGTCCGGCCGGCAGGCCGCGGCGCTG
GACCTCTACCGCAAGCTGCGGGTACGGCTCATCGAGGAGCTGGGCGTGGAGCCCTCGCCG
CAGATGCAGCGCATGCACCAGGCGATGCTCTCCGTGGACCCCGCGCTCGACCTGGCCACG
GGGCCCCGGCGCGGCTCGACGTTCGACCTGTACGCGGCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001867 Signal transduction response regulator, C-terminal (Domain) SM00862 Trans_reg_C PF00486 Trans_reg_C IPR005158 Bacterial transcriptional activator domain (Domain) PF03704 BTAD SM01043 BTAD IPR011990 Tetratricopeptide-like helical (Domain) G3DSA:1.25.40.10 TPR-like_helical IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR016032 Signal transduction response regulator, C-terminal effector (Domain) SSF46894 Bipartite_resp_reg_C-effector
SignalP	No significant hit
TMHMM	No significant hit

Griseo_00010 Griseo_00030