Griseo_00270 Griseo_00270   Griseo_00290 Griseo_00290

Griseo_00280 : CDS information

close this sectionLocation

Organism
StrainJP95
Entry nameGriseorhodin A
Contig
Start / Stop / Direction30,227 / 31,507 / + [in whole cluster]
30,227 / 31,507 / + [in contig]
Location30227..31507 [in whole cluster]
30227..31507 [in contig]
TypeCDS
Length1,281 bp (426 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase beta-ketoacyl synthase subunit
Product (GenBank)putative ketosynthase alpha GrhA
Gene
Gene (GenBank)grhA
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
Reference
ACC
PmId
[12323376] A gene cluster from a marine Streptomyces encoding the biosynthesis of the aromatic spiroketal polyketide griseorhodin A. (Chem Biol. , 2002)
comment
griseorhodin A生合成gene clusterのクローニング、シークエンス、異種性発現。

GrhA: KS-alpha
配列解析のみ。

---
http://hss.ulb.uni-bonn.de/2012/2790/2790.htm
"Investigations on the early biosynthetic steps of griseorhodin A" (学位論文)

grhA, grhB, grhC, grhE, grhT, grhQをクローニングして、S.albusでの異種性発現に利用しているが、うまくいっていない。

grh cluster内cyclases GrhT or GrhQのdeletionは生合成を廃止し、polyketidesは検出されなかった。
ΔgrhH-P mutantでは早期中間体形成が見られたが、ΔgrhH-Q mutantではgriseorhodin A生合成がなかった。

これらの結果から、griseorhodin A生合成における早期のpolyketide組み立てには多酵素複合体形成が必要であると述べている。
Related Reference
ACC
Q54173
NITE
Urd_00120
PmId
[7592377] Cloning and characterization of a polyketide synthase gene from Streptomyces fradiae Tu2717, which carries the genes for biosynthesis of the angucycline antibiotic urdamycin A and a gene probably involved in its oxygenation. (J Bacteriol. , 1995)
comment
Blast 9th, id71%, 1e-172
Streptomyces fradiae_urdA
Putative ketoacyl synthase

urdEFABCDを含むcosmid purd8をS.lividans TK24へ導入すると、urdamycin抵抗性を獲得。
urdamycin産生株S.fradiae Tu2717染色体から、urdE-C末+urdFABを含むfragment g領域を削除するとurdamycin産生不可。

AA配列解析で、UrdAはPKS gene clustersのbeta-ketoacyl synthasesに高い類似性あり。
UrdAとUrdBはid36%で似ているが、UrdBにはないactive site cysteineをUrdAは持っている。

TcmK ketoacyl synthase or TcmL chain length factorのどちらかを障害してtetracenomycin C非産生にしたStreptomyces glaucescensのmutantで、urdFABを異種性発現したらどちらのmutantも相補され、tetracenomycin Cを産生できた。

close this sectionPKS/NRPS Module

KS3..375

close this sectionSequence

selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [putative ketosynthase alpha GrhA]
MSRRAVITGIGAVAPGGIGRDAYWDLLAHGRTATRRISRFDASAFRSQVAAECDFDPVAA
GLSPQEVRRMDRAAQFAVVAAQEAIEDSGIDVGAGDPGRTAVSIGSAVGCTTGLEDEYAV
LSDGGRNWLVDHTYGVPHLYGYMVPSTLAAEVAWRARAEGPVALISTGCTSGLDAIAHGV
QLIEEGAADIVLAGATDAPLSPITSACFDAIKATTPNADQDPGSASRPFDGRRDGFVLGE
GSAVMVIEEAGAARRRGATVYAEVAGFANRSNAFHMTGLKPDGREMAEAIRVALDRARLD
PGAIDHVNAHGSGTKQNDRHETAAFKRSLGQRAHEIPVSSIKSMIGHSLGAIGSLEVAAC
ALALRHQVVPPTANLTVPDPQCDLDYVPVTAREHRMDHVLSVGSGFGGFQSAMILSRPGA
RKEAVA
selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [putative ketosynthase alpha GrhA]
GTGAGCCGACGCGCCGTCATCACGGGGATCGGAGCCGTCGCTCCCGGCGGCATCGGACGG
GACGCGTACTGGGACCTGCTCGCGCACGGCCGTACGGCCACCCGGCGGATCAGCCGGTTC
GACGCGTCGGCGTTCCGCTCGCAGGTCGCCGCCGAATGCGACTTCGACCCGGTCGCCGCG
GGGCTGAGCCCGCAGGAAGTGCGCCGGATGGACCGGGCCGCCCAGTTCGCGGTGGTCGCG
GCCCAGGAGGCGATCGAGGACAGCGGCATCGACGTCGGCGCGGGCGACCCGGGGCGGACC
GCGGTCAGCATCGGCAGCGCGGTCGGCTGCACCACCGGCCTGGAGGACGAGTACGCGGTC
CTCTCGGACGGCGGCCGGAACTGGCTGGTCGACCACACCTACGGGGTCCCGCACCTGTAC
GGCTACATGGTCCCCAGCACGCTGGCGGCCGAAGTGGCCTGGCGGGCGCGGGCGGAGGGC
CCGGTGGCACTGATCTCGACCGGCTGCACCTCCGGTCTCGACGCGATCGCCCACGGCGTG
CAGCTCATCGAGGAGGGCGCCGCCGACATCGTCCTGGCGGGCGCCACCGACGCCCCGCTG
TCCCCCATCACCTCCGCCTGCTTCGACGCGATCAAGGCGACGACGCCCAACGCGGACCAG
GACCCGGGCAGCGCCTCCCGCCCCTTCGACGGCCGCCGCGACGGCTTCGTGCTGGGCGAG
GGCTCGGCGGTCATGGTGATCGAGGAGGCCGGGGCGGCCCGCCGCCGGGGGGCCACCGTC
TACGCCGAGGTGGCCGGTTTCGCCAACCGCTCCAACGCCTTCCACATGACCGGGCTCAAG
CCCGACGGGCGGGAGATGGCCGAGGCCATCCGGGTCGCCCTGGACCGCGCGCGGCTCGAC
CCCGGCGCGATCGACCACGTCAACGCGCACGGCTCCGGCACCAAGCAGAACGACCGGCAC
GAGACGGCCGCCTTCAAGCGGTCCCTGGGGCAGCGCGCCCACGAGATCCCGGTCAGCTCC
ATCAAGTCGATGATCGGGCACTCCCTGGGGGCGATCGGCTCCCTGGAGGTGGCCGCCTGC
GCCCTGGCGCTGCGCCACCAGGTGGTGCCGCCCACGGCGAACCTCACGGTCCCCGACCCC
CAGTGCGACCTGGACTACGTCCCGGTCACCGCCCGGGAGCACCGCATGGACCACGTCCTC
AGCGTCGGCAGCGGATTCGGCGGCTTCCAGAGCGCCATGATCCTCAGCCGGCCCGGAGCC
CGTAAGGAGGCGGTCGCGTGA
KS3..375
KS7..1125

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[3-250] 6.59999999999996e-59 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[261-375] 1.29999999999998e-38 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[7-266] 4.6e-62 G3DSA:3.40.47.10 [271-417] 1.80000000000002e-53 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[2-420] 8.30001403791594e-64 SSF53901
SSF53901   Thiolase-like
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[160-176] PS00606
PS00606   B_KETOACYL_SYNTHASE
SignalP
[1-19] 0.725 Signal
Eukaryota   
TMHMM No significant hit
Griseo_00270 Griseo_00270   Griseo_00290 Griseo_00290
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