Halsto_00070 Halsto_00070   Halsto_00090 Halsto_00090

Halsto_00080 : CDS information

close this sectionLocation

Organism
StrainHC34
Entry nameHalstoctacosanolide
Contig
Start / Stop / Direction100,132 / 101,346 / + [in whole cluster]
100,132 / 101,346 / + [in contig]
Location100132..101346 [in whole cluster]
100132..101346 [in contig]
TypeCDS
Length1,215 bp (404 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productcytochrome P450
Product (GenBank)cytochrome P450 monooxygenase
Gene
Gene (GenBank)hlsH
EC number
Keyword
  • C-4 keto group formation
Note
Note (GenBank)
Reference
ACC
PmId
[16568718] The complete biosynthetic gene cluster of the 28-membered polyketide macrolactones, halstoctacosanolides, from Streptomyces halstedii HC34. (J Antibiot (Tokyo). , 2006)
comment
halstoctacosanolide生合成に関与するgene clusterのクローニングを行い、完全長を決定した文献。
hlsH(404aa): Cytochrome P450 monooxygenase

また、hlsH, hlsIそれぞれの欠損変異株を作製して、各々の機能を明らかにしている。
Halstoctacosanolide B より Halstoctacosanolide A への変換をHlsH が司る。
（Halstoctacosanolide B の C-4 methylene group を酸化して carbonyl化）

close this sectionSequence

selected fasta
>cytochrome P450 [cytochrome P450 monooxygenase]
MTEATQTGTCPHFPFSNGRPVELEPEYIEFRRDRPVARVELPYGGEAWLLTRMAEVKEFL
GDTRFSRQAMKGFSPRYTEAPIADEGGLPMMDGAQHLRTRRLLNKAFSRRRVETLRPAVT
AIVNDLVDAMEAGERPVDLVAEFGNAIPREVNCLLLGVPSEDWSRLGRLVDDVIHQGVIP
QEQLIASWTELTGYFMTMIKDRRTTPTEDLISEMIQARNGEDVFADEEVMMNAVVVFVAG
YETTACQIVNMICALLRDPDQMKWLGENLDALPQAVEELLRYVPLTAAGVMPSIAAEDAE
IGGVPISAGEAVYTCVVSANRDEEVFTEPEKLNLRRGDEVNQHVAFGAGPHYCLGASLAR
VELQVALEAVLRRMPGIRLAIPEAELPLKDGLVLRGFEQFPVVW
selected fasta
>cytochrome P450 [cytochrome P450 monooxygenase]
GTGACCGAAGCCACCCAGACCGGCACCTGCCCGCATTTCCCGTTCAGCAACGGACGGCCG
GTGGAACTGGAGCCCGAGTACATCGAGTTCCGGCGGGACAGACCCGTCGCGCGTGTCGAG
CTGCCGTACGGGGGCGAGGCGTGGCTGCTCACCAGGATGGCCGAGGTGAAGGAGTTCCTC
GGTGACACCCGGTTCAGCCGCCAGGCGATGAAGGGCTTCAGCCCCCGCTACACCGAGGCG
CCGATCGCGGACGAGGGCGGCCTCCCGATGATGGACGGCGCCCAGCACCTCCGCACCCGG
CGTCTCCTCAACAAGGCGTTCAGCCGCCGTCGGGTCGAGACCCTGCGGCCCGCCGTCACC
GCGATCGTGAACGACCTGGTCGACGCGATGGAGGCGGGCGAGCGCCCGGTGGACCTCGTC
GCCGAGTTCGGCAACGCGATACCCCGTGAGGTGAACTGCCTGCTGCTCGGTGTGCCCAGC
GAGGACTGGAGCAGGCTGGGCCGGCTCGTGGACGACGTCATCCACCAGGGCGTGATCCCC
CAGGAGCAGTTGATCGCCTCCTGGACCGAGCTGACCGGCTACTTCATGACCATGATCAAG
GACCGTCGCACCACCCCGACGGAGGACCTGATCAGCGAGATGATCCAAGCCAGAAACGGC
GAGGACGTGTTCGCCGACGAAGAAGTCATGATGAACGCCGTGGTCGTCTTTGTCGCTGGG
TACGAGACCACCGCCTGCCAGATCGTCAACATGATCTGCGCCCTGCTGCGCGACCCCGAC
CAGATGAAGTGGCTCGGAGAGAACCTGGACGCCCTGCCGCAGGCGGTGGAGGAACTGCTC
CGCTATGTGCCGCTAACCGCGGCCGGTGTGATGCCGTCGATAGCCGCCGAGGACGCGGAG
ATCGGCGGGGTGCCGATCTCCGCCGGAGAGGCGGTCTACACCTGCGTGGTCTCGGCCAAC
CGCGACGAGGAGGTCTTCACCGAGCCCGAGAAGCTGAATCTGCGGCGGGGCGACGAGGTG
AACCAGCACGTGGCCTTCGGCGCCGGTCCGCACTACTGCCTCGGTGCCTCCCTCGCCCGG
GTCGAGCTCCAGGTGGCGCTGGAGGCCGTGCTGCGCCGGATGCCCGGTATCCGACTGGCC
ATTCCCGAGGCCGAGCTGCCGCTGAAGGACGGTCTGGTGCTGCGCGGCTTCGAGCAGTTC
CCGGTCGTCTGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[239-256] 2.00000075217457e-07 PR00385 [274-285] 2.00000075217457e-07 PR00385 [344-353] 2.00000075217457e-07 PR00385 [353-364] 2.00000075217457e-07 PR00385
PR00385   P450
[10-404] 1.50000965748778e-89 SSF48264
SSF48264   Cytochrome_P450
[22-404] 8.1e-101 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
[188-373] 1.2e-26 PF00067
PF00067   p450
IPR002397 Cytochrome P450, B-class (Family)
[91-102] 1.10000150671643e-44 PR00359 [138-154] 1.10000150671643e-44 PR00359 [155-170] 1.10000150671643e-44 PR00359 [193-215] 1.10000150671643e-44 PR00359 [274-285] 1.10000150671643e-44 PR00359 [293-320] 1.10000150671643e-44 PR00359 [321-336] 1.10000150671643e-44 PR00359 [344-353] 1.10000150671643e-44 PR00359 [353-364] 1.10000150671643e-44 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[346-355] PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit
Halsto_00070 Halsto_00070   Halsto_00090 Halsto_00090
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