Halsto_00080 Halsto_00080   last last

Halsto_00090 : CDS information

close this sectionLocation

Organism
StrainHC34
Entry nameHalstoctacosanolide
Contig
Start / Stop / Direction101,343 / 102,587 / + [in whole cluster]
101,343 / 102,587 / + [in contig]
Location101343..102587 [in whole cluster]
101343..102587 [in contig]
TypeCDS
Length1,245 bp (414 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productcytochrome P450
Product (GenBank)cytochrome P450 monooxygenase
Gene
Gene (GenBank)hlsI
EC number
Keyword
  • C-32 hydroxylate
Note
Note (GenBank)
Reference
ACC
PmId
[16568718] The complete biosynthetic gene cluster of the 28-membered polyketide macrolactones, halstoctacosanolides, from Streptomyces halstedii HC34. (J Antibiot (Tokyo). , 2006)
comment
halstoctacosanolide生合成に関与するgene clusterのクローニングを行い、完全長を決定した文献。
hlsI (414aa): Cytochrome P450 monooxygenase

また、hlsH, hlsI それぞれの欠損変異株を作製して、各々の機能を明らかにしている。
Halstoctacosanolide C より Halstoctacosanolide B への変換をHlsI が司る。
（C-32の methylene group を hydroxyl 化)

close this sectionSequence

selected fasta
>cytochrome P450 [cytochrome P450 monooxygenase]
MTAGTTGTPAGGSRSGTEQAPDFPFADENGIQLGTEYDPLLARDGLARVTMPHGGDAWIA
TRMADVKKVLSDPRLSRTAAARPDSPRYTPEVLPSGGVLVGLDGPEHARIRRLLGKALSV
GLMEGMRPWIRETVDEILTDLLDQGPPVDLVRELGEKLPRLAICKLLGMPYSDRHRFDES
VDVVVSMATRSRDEVGRAWSTLSAYIGELVEAKRREPAEDLISALLEVRHDGDSFSDREV
ITNTIAVLVAGYESTASELTTMAYLLMVHPGLKDSLREDPSRIPASVEEMLRYAALSPAG
AMTCRATEDVTIGETEVREGDAVVPCLVAANYDQTVFDQPRELDPDRGNKGKHLAFGFGA
HYCMGARLARIELQEALTGLVHRMPGLRLAVSEKLLPRRTEMGMRGFLRLPVTW
selected fasta
>cytochrome P450 [cytochrome P450 monooxygenase]
GTGACCGCCGGTACGACAGGGACGCCGGCAGGGGGGTCCCGGTCCGGTACCGAGCAGGCG
CCCGACTTCCCCTTCGCCGACGAGAACGGAATCCAACTCGGCACCGAGTACGATCCGCTG
CTCGCCCGGGACGGCCTCGCCCGGGTGACCATGCCGCACGGCGGTGATGCCTGGATCGCA
ACCCGGATGGCCGATGTCAAGAAGGTGCTCTCCGACCCCCGGCTGAGCCGGACCGCGGCC
GCCCGCCCGGACTCGCCGCGCTACACCCCGGAGGTGCTGCCCAGCGGCGGTGTCCTGGTG
GGACTCGACGGGCCCGAGCACGCCCGGATCCGCCGGCTGCTCGGCAAGGCGCTCTCGGTC
GGCCTCATGGAGGGCATGAGGCCGTGGATCCGGGAGACGGTGGACGAGATCCTGACCGAT
CTGCTGGACCAGGGCCCGCCGGTGGACCTGGTGCGGGAGCTGGGGGAGAAGCTGCCCCGA
CTGGCCATCTGCAAGCTGCTCGGTATGCCTTACAGCGATCGGCACCGGTTCGACGAATCG
GTGGATGTGGTGGTCTCCATGGCCACCCGCTCGCGGGACGAGGTCGGCCGCGCCTGGAGC
ACCCTCTCCGCGTACATCGGCGAGCTGGTCGAGGCCAAGCGCCGGGAGCCCGCCGAGGAC
CTGATCTCCGCACTGCTGGAGGTACGGCACGACGGCGATTCCTTCTCCGACCGGGAGGTG
ATCACCAACACGATCGCCGTACTGGTCGCCGGGTACGAGAGCACGGCTTCAGAGCTGACC
ACCATGGCCTATCTGCTGATGGTCCACCCTGGCCTGAAGGACAGCCTCCGGGAGGACCCG
TCGCGGATCCCGGCCTCCGTGGAGGAGATGCTGCGCTATGCCGCGCTCAGCCCGGCCGGC
GCCATGACCTGCCGAGCCACCGAGGACGTGACGATCGGGGAGACGGAGGTCCGCGAGGGG
GACGCGGTGGTGCCCTGTCTGGTCGCGGCCAACTACGACCAGACGGTCTTCGACCAACCC
CGGGAGCTGGACCCGGACCGGGGCAACAAGGGCAAGCACCTCGCGTTCGGCTTCGGCGCC
CACTACTGCATGGGCGCCCGGCTGGCCCGGATCGAACTCCAGGAGGCACTGACCGGACTG
GTCCACCGGATGCCCGGACTGCGGCTCGCGGTCAGCGAGAAGCTGCTGCCGCGCCGCACC
GAGATGGGGATGCGTGGGTTCCTCCGACTGCCGGTGACCTGGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[172-382] 2.4e-25 PF00067
PF00067   p450
[250-267] 2.4000006166714e-07 PR00385 [285-296] 2.4000006166714e-07 PR00385 [354-363] 2.4000006166714e-07 PR00385 [363-374] 2.4000006166714e-07 PR00385
PR00385   P450
[36-414] 1.69999999999998e-95 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
[10-414] 2.39999798157263e-88 SSF48264
SSF48264   Cytochrome_P450
IPR002397 Cytochrome P450, B-class (Family)
[102-113] 3.29999077503323e-46 PR00359 [149-165] 3.29999077503323e-46 PR00359 [166-181] 3.29999077503323e-46 PR00359 [204-226] 3.29999077503323e-46 PR00359 [285-296] 3.29999077503323e-46 PR00359 [304-331] 3.29999077503323e-46 PR00359 [332-347] 3.29999077503323e-46 PR00359 [354-363] 3.29999077503323e-46 PR00359 [363-374] 3.29999077503323e-46 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[356-365] PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit
Halsto_00080 Halsto_00080   last last
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