Heda_00080 Heda_00080   Heda_00100 Heda_00100

Heda_00090 : CDS information

close this sectionLocation

Organism
StrainATCC 15422
Entry nameHedamycin
Contig
Start / Stop / Direction17,084 / 18,130 / + [in whole cluster]
17,084 / 18,130 / + [in contig]
Location17084..18130 [in whole cluster]
17084..18130 [in contig]
TypeCDS
Length1,047 bp (348 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative DeoR family transcriptional regulator
Product (GenBank)transcription repressor
GeneorfH9
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • orf24
Reference
ACC
PmId
[15271354] The hedamycin locus implicates a novel aromatic PKS priming mechanism. (Chem Biol. , 2004)
comment
hedamycinの生合成gene clusterの報告。

orfH9: Transcription repressor

配列解析のみ。
transcriptional repressor proteinであると提唱されているDnrOの近いhomologである。
Related Reference
ACC
Q54822
NITE
Adria_00110
PmId
[10846224] The dnrO gene encodes a DNA-binding protein that regulates daunorubicin production in Streptomyces peucetius by controlling expression of the dnrN pseudo response regulator gene. (Microbiology. , 2000)
[20122958] The activator/repressor protein DnrO of Streptomyces peucetius binds to DNA without changing its topology. (Int J Biol Macromol. , 2010)
[20529134] Modulation of dnrN expression by intracellular levels of DnrO and daunorubicin in Streptomyces peucetius. (FEMS Microbiol Lett. , 2010)
[20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010)
comment
Blast 7th, id54%, 6e-86
Streptomyces peucetius_dnrO
Putative repressor
[Adria_00110]transcriptional activator/repressor

---
[PMID: 10846224](2000)
dnrO::aphII mutant strainはDNR生合成不可能。
このmutant strainにdnrN or dnrI を導入すると生合成可能。
dnrI 発現はDnrNが必要なことがわかっているので、dnrN発現にDnrOが必要ということが示される。

DnrOをE.coliで過剰発現し、抽出液でmobility shift DNA-binding assays。
DnrOはoverlapping dnrN/dnrO(p1) promoter regionへ特異的に結合した。

---
その後、別グループでも報告あり。
[PMID: 20122958][PMID: 20529134][PMID: 20158521](2010)
dnrO, dnrN, dnrI, DNRが関与する細胞内DNR濃度維持システムが提唱されている。
DnrOはDnrNと競合してfeedback的にDNRの細胞内濃度を調節している。

close this sectionSequence

selected fasta
>putative DeoR family transcriptional regulator [transcription repressor]
MLETSARLLRLLALLQARHEWGGAELAERLSVSPRTVRRDVDKLRALGYPIQSIGGVGGG
YQLAAGASLPPLLLDDEEAVAVAVGLRTAAAGTVTGIAETSTRALAKLDQVLPSRLRARV
NAISSALVTLPMLGSTVDPGALTAIATAIRTHERLRFDYRDGRGVSEERDAEPYRLVHNE
RRWYLFAWDTARAGWRTFRVDRMRLRIPNGPRFRPRPLPGEGVAEYVTLSMTTTRHRYHA
VLTMHGNARDVADEVPASLGLVEPVDEHTCTLRIGSDSLDQLAVWVAAFGFDFEVHEPPE
LVAHLRTLTARLSRAADASAAGPGRAPAGRADRGQTSPNEAATARARA
selected fasta
>putative DeoR family transcriptional regulator [transcription repressor]
ATGCTGGAGACCTCGGCACGGCTGCTGCGACTACTGGCCCTGCTCCAGGCGCGCCACGAG
TGGGGAGGCGCCGAACTGGCCGAACGCCTGTCGGTGAGCCCCCGCACGGTGCGCCGGGAC
GTCGACAAGCTGCGCGCCCTCGGCTATCCGATCCAGTCCATCGGCGGCGTGGGCGGCGGG
TACCAGTTGGCGGCGGGCGCCTCGCTGCCACCGCTCCTCCTCGACGACGAGGAGGCCGTC
GCGGTGGCCGTGGGTCTGCGCACCGCCGCCGCAGGCACGGTGACCGGCATAGCCGAGACC
TCGACGCGGGCGCTGGCCAAGCTCGACCAGGTGCTGCCCTCGCGGCTGCGGGCCCGCGTC
AACGCCATCAGCTCCGCCCTGGTCACCCTGCCCATGCTCGGCTCCACCGTCGACCCGGGC
GCGCTGACCGCGATCGCCACGGCGATCCGGACCCACGAGCGGCTGCGCTTCGACTACCGG
GACGGCCGCGGCGTGTCCGAGGAGCGCGACGCCGAACCGTACCGGCTCGTGCACAACGAG
CGCCGCTGGTACCTGTTCGCCTGGGACACCGCGCGGGCCGGCTGGCGGACCTTCCGGGTG
GACCGGATGCGGCTGCGCATCCCCAACGGGCCCCGGTTCCGTCCCCGGCCACTGCCCGGG
GAGGGCGTCGCCGAGTACGTCACGCTGAGCATGACGACCACCCGCCACCGCTACCACGCC
GTCCTCACCATGCACGGCAACGCCCGCGACGTGGCCGACGAGGTCCCCGCCTCGCTGGGC
CTGGTGGAACCGGTCGACGAGCACACCTGCACCCTGCGGATCGGGTCCGACAGCCTCGAC
CAGCTCGCGGTGTGGGTGGCCGCGTTCGGCTTCGACTTCGAGGTCCACGAGCCACCGGAA
CTCGTCGCCCACCTGCGCACCCTGACCGCCCGGCTGAGCCGGGCGGCCGACGCGAGCGCC
GCCGGCCCGGGCCGGGCGCCGGCGGGACGCGCCGACCGCGGTCAGACCTCGCCGAACGAG
GCCGCCACCGCGCGGGCCCGGGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001034 HTH DeoR N-terminal domain (Domain)
[4-59] PS51000
PS51000   HTH_DEOR_2
[7-59] 6.899999633797e-05 SM00420
SM00420   HTH_DEOR
[25-39] 6.59999852526375e-05 PR00037 [39-57] 6.59999852526375e-05 PR00037
PR00037   HTHLACR
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[2-65] 4.70000000000001e-18 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR013196 Helix-turn-helix, type 11 (Domain)
[7-62] 1.3e-15 PF08279
PF08279   HTH_11
IPR018356 Transcription regulator, HTH DeoR-type, conserved site (Conserved_site)
[7-41] PS00894
PS00894   HTH_DEOR_1
SignalP
[1-26] 0.646 Signal
Eukaryota   
TMHMM No significant hit
Heda_00080 Heda_00080   Heda_00100 Heda_00100
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