Heda_00090 Heda_00090   Heda_00110 Heda_00110

Heda_00100 : CDS information

close this sectionLocation

Organism
StrainATCC 15422
Entry nameHedamycin
Contig
Start / Stop / Direction18,735 / 18,085 / - [in whole cluster]
18,735 / 18,085 / - [in contig]
Locationcomplement(18085..18735) [in whole cluster]
complement(18085..18735) [in contig]
TypeCDS
Length651 bp (216 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative oxidoreductase
Product (GenBank)ACP-phosphodiesterase
GeneorfH8
Gene (GenBank)
EC number
Keyword
Note
  • The N-terminal position was modified from original INSDC entry.
Note (GenBank)
  • orf23
Reference
ACC
PmId
[15271354] The hedamycin locus implicates a novel aromatic PKS priming mechanism. (Chem Biol. , 2004)
comment
hedamycinの生合成gene clusterの報告。

orfH8: ACP-phosphodiesterase

このORFについて詳細記載なし。
Related Reference
ACC
P41407
PmId
comment
Blast 228th, id33%, 6e-16
E.coli_azoR(acpD)
FMN-dependent NADH-azoreductase

E.coliでの実験いままでACP-phosphodiesteraseだと考えられてきたが、aromatic azo化合物のアゾ基を分解する活性を持つことを報告した論文。2004年には構造解析も出ている。

close this sectionSequence

selected fasta
>putative oxidoreductase [ACP-phosphodiesterase]
MARLLHIDSSSARDSVSRALAVRFREVWDKEHGPDSVTYRDLVADPVPHLDGDAVVTLWS
EPYTEAQRATATLQNRLVEEILAADALLVSVPMYNWTVPSGLKAWLDQSLILGRTLPYDP
GHKPLGGRPATVLTACGGYYGADVPEASMDHCRPYLATVLGQVLGYDLETITVWRTLATA
GQGDARERRAAEESLRAGQEAAQARARAVAASFGEV
selected fasta
>putative oxidoreductase [ACP-phosphodiesterase]
ATGGCCCGACTGCTGCACATCGACTCGTCCTCCGCCCGGGACTCCGTCTCCCGCGCGCTC
GCCGTCCGCTTCCGTGAGGTGTGGGACAAGGAACACGGGCCCGACTCCGTGACCTACCGC
GACCTGGTCGCCGACCCCGTCCCGCACCTCGACGGGGACGCGGTCGTCACCCTGTGGTCC
GAGCCGTACACCGAAGCCCAGCGGGCGACGGCGACGCTGCAGAACCGGCTGGTGGAGGAG
ATCCTGGCCGCGGACGCGCTGCTGGTCAGCGTGCCGATGTACAACTGGACCGTGCCCTCG
GGGCTCAAGGCGTGGCTGGACCAGTCGCTGATCCTGGGCCGCACCCTGCCCTACGACCCG
GGCCACAAGCCGCTGGGCGGGCGCCCGGCGACCGTGCTGACGGCCTGCGGCGGCTACTAC
GGCGCGGACGTGCCGGAGGCGTCCATGGACCACTGCCGTCCCTACCTCGCCACCGTGCTG
GGTCAGGTGCTCGGCTACGACCTGGAGACGATCACGGTGTGGCGCACCCTGGCCACCGCA
GGCCAGGGCGACGCGCGGGAGCGCCGGGCGGCCGAGGAGTCGCTGCGCGCCGGCCAGGAG
GCGGCTCAGGCCCGGGCCCGCGCGGTGGCGGCCTCGTTCGGCGAGGTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003680 Flavodoxin-like fold (Domain)
[3-182] 1.9e-30 PF02525
PF02525   Flavodoxin_2
IPR023048 NADH-azoreductase, FMN-dependent (Family)
[2-210] 24.671 MF_01216
MF_01216   Azoreductase_type1
SignalP
[1-21] 0.128 Signal
Eukaryota   
TMHMM No significant hit
Heda_00090 Heda_00090   Heda_00110 Heda_00110
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