Heda_00160 Heda_00160   Heda_00180 Heda_00180

Heda_00170 : CDS information

close this sectionLocation

Organism
StrainATCC 15422
Entry nameHedamycin
Contig
Start / Stop / Direction27,197 / 26,379 / - [in whole cluster]
27,197 / 26,379 / - [in contig]
Locationcomplement(26379..27197) [in whole cluster]
complement(26379..27197) [in contig]
TypeCDS
Length819 bp (272 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative transcriptional regulator
Product (GenBank)putative regulatory protein
GeneorfH5
Gene (GenBank)
EC number
Keyword
  • SARP family
Note
Note (GenBank)
  • orf16
Reference
ACC
PmId
[15271354] The hedamycin locus implicates a novel aromatic PKS priming mechanism. (Chem Biol. , 2004)
comment
hedamycinの生合成gene clusterの報告。

orfH5: Regulatory protein

配列解析のみ。
SARP familyのtranscriptional activatorsに似ている。
Related Reference
ACC
P25047
NITE
Adria_00210
PmId
[1729206] Regulation of secondary metabolism in Streptomyces spp. and overproduction of daunorubicin in Streptomyces peucetius. (J Bacteriol. , 1992)
[7868593] Functional characterization and transcriptional analysis of the dnrR1 locus, which controls daunorubicin biosynthesis in Streptomyces peucetius. (J Bacteriol. , 1995)
[8971703] Purification and characterization of the DNA-binding protein DnrI, a transcriptional factor of daunorubicin biosynthesis in Streptomyces peucetius. (Mol Microbiol. , 1996)
[16545946] Feedback regulation of doxorubicin biosynthesis in Streptomyces peucetius. (Res Microbiol. , 2006)
[20529134] Modulation of dnrN expression by intracellular levels of DnrO and daunorubicin in Streptomyces peucetius. (FEMS Microbiol Lett. , 2010)
[20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010)
comment
Blast 4th, id53%, 2e-68
Streptomyces peucetius_dnrI
Regulatory protein dnrI
[Adria_00210]transcriptional activator

--
[PMID: 1729206](1992)
ATCC 29050 strainでdnrI を不活化したら、RHO産生とdaunorubicin (DNR)産生ができなくなり、DNRへの抵抗性が著しく減った。

---
同じ著者らの続報。
[PMID: 7868593](1995)
dnrI::aphII mutantでは、DNR生合成におけるearly- and late-acting enzymesの両方をコードする転写物が存在しない。よってdnrI は、直接または間接的にDNR生合成遺伝子のすべてを調節している。

dnrI::aphII and dnrN::aphII strainsでresistance genes=drrAB and drrC転写物は消失。
よって、dnrI はDNR抵抗性の重大なregulatorであり、dnrNはdnrI を通して間接的にその影響を発現する、と示唆される。

--
[PMID: 8971703](1996) abstract
DNA-binding assays.
・dnrG-dpsABCD と dpsEF の間
・dnrC gene と dnrDKPSQ の間
の遺伝子間領域を含んだDNA断片へ特異的に結合する。

--
[PMID: 16545946](2006) abstract
dnrI promoter活性を増やすためにはDnrO and DnrN の両方が必要であった。

--
[PMID: 20529134]や[PMID: 20158521]で、dnrO, dnrN, dnrI, DNRが関与する細胞内DNR濃度維持システムが提唱されている。
dnrI promoterにはDnrNが結合し活性化される。

close this sectionSequence

selected fasta
>putative transcriptional regulator [putative regulatory protein]
MRIRLLGELEAVLNDVSITPTAAKPRQVLALLALNARRIVSVQSLVEELWGHHPPPSVRT
TLQTYILQLRKLIDSALPAGSGRTAKDILVTRFDGYLLDIVPENVDVCSYERLVKAGQQA
LARGDHEAVSTLLGMALTKWQSRALVDVRIGLLLSLEVARLEESRLNALEARIAADIQLG
RHQSVLSELTVLTAQQPMHENLCAHYMVSLYRSGRQWQALQAYKTLRTTLNRELGVEPSL
RLRRLQQAVLRSEADLDVLVEPVPDPARATNA
selected fasta
>putative transcriptional regulator [putative regulatory protein]
ATGCGCATACGACTTCTGGGAGAACTGGAAGCCGTTCTCAACGATGTCTCAATCACACCG
ACCGCGGCGAAACCCCGCCAAGTACTGGCCCTCCTGGCGCTCAACGCCAGGCGAATCGTT
TCCGTGCAAAGTCTCGTCGAAGAGCTGTGGGGCCACCACCCGCCGCCGAGCGTGCGGACC
ACTCTCCAGACGTACATCCTCCAACTGCGCAAACTCATCGACTCCGCGCTCCCCGCGGGC
TCCGGACGCACCGCCAAGGACATCCTGGTCACCCGGTTCGACGGGTACCTCCTCGACATC
GTCCCGGAGAACGTCGACGTCTGCTCCTACGAACGCCTCGTCAAGGCCGGTCAGCAGGCG
CTGGCGAGGGGAGACCACGAGGCGGTCTCGACCCTGCTCGGCATGGCTCTGACCAAGTGG
CAGAGCCGCGCCCTGGTGGACGTGCGCATCGGCCTGCTGCTCTCCCTGGAGGTGGCGCGG
CTGGAGGAGAGCCGCCTGAACGCCCTGGAGGCCAGGATCGCCGCCGACATCCAGCTCGGC
CGGCACCAGTCGGTGCTCAGCGAGCTGACCGTGCTCACCGCCCAGCAGCCCATGCACGAG
AACCTGTGCGCCCACTACATGGTCAGCCTCTACCGCTCCGGCCGGCAGTGGCAGGCGTTG
CAGGCCTACAAGACGCTGCGCACCACCCTGAACCGCGAACTGGGCGTAGAGCCCTCGCTC
CGGCTGCGCCGGCTGCAGCAGGCGGTGCTGCGCTCGGAGGCGGACCTCGACGTGCTCGTG
GAGCCGGTCCCCGACCCCGCGCGAGCCACCAACGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001867 Signal transduction response regulator, C-terminal (Domain)
[20-98] 1.2e-18 PF00486
PF00486   Trans_reg_C
[15-98] 8.49999291746323e-11 SM00862
SM00862   Trans_reg_C
IPR005158 Bacterial transcriptional activator domain (Domain)
[105-250] 3.40000000000004e-46 PF03704
PF03704   BTAD
[105-250] 2.9000035467798e-45 SM01043
SM01043   BTAD
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[23-98] 4.5e-08 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016032 Signal transduction response regulator, C-terminal effector (Domain)
[1-99] 8.60000664726925e-14 SSF46894
SSF46894   Bipartite_resp_reg_C-effector
SignalP
[1-44] 0.162 Signal
Eukaryota   
TMHMM No significant hit
Heda_00160 Heda_00160   Heda_00180 Heda_00180
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