Heda_00180 Heda_00180   Heda_00200 Heda_00200

Heda_00190 : CDS information

close this sectionLocation

Organism
StrainATCC 15422
Entry nameHedamycin
Contig
Start / Stop / Direction28,743 / 29,423 / + [in whole cluster]
28,743 / 29,423 / + [in contig]
Location28743..29423 [in whole cluster]
28743..29423 [in contig]
TypeCDS
Length681 bp (226 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose epimerase
Product (GenBank)putative 3-5-epimerase
GenehedK
Gene (GenBank)
EC number5.1.3.-
Keyword
  • alpha-L-N,N-dimethylvancosamine
Note
Note (GenBank)
  • orf14
Reference
ACC
PmId
[15271354] The hedamycin locus implicates a novel aromatic PKS priming mechanism. (Chem Biol. , 2004)
comment
hedamycinの生合成gene clusterの報告。

hedK: 3,5-Epimerase

配列解析のみ。
alpha-L-N,N-dimethylvancosamine (3-dimethylamino-2,3,6-trideoxy-3-C-methyl-alpha-L-lyxo-hexopyranose)の生合成での異性化に割り当てられている。
参照しているのはvancomycin gene clusterでのdTDP-L-vancosamine生合成経路。
Related Reference
ACC
Q9S0P2
NITE
Aver_00150
PmId
[11451669] Insights about the biosynthesis of the avermectin deoxysugar L-oleandrose through heterologous expression of Streptomyces avermitilis deoxysugar genes in Streptomyces lividans. (Chem Biol. , 2001)
comment
Blast 17th, id59%, 6e-58
Streptomyces avermitilis_aveBV(avrF)
dTDP-4-keto-6-deoxyhexose 3,5-epimerase
[Aver_00150]NDP-hexose 5-epimerase

Table1.
avrF/aveBV: dTDP-4-keto-6-deoxyglucose 3-epimerase　
(本文を読むと5-epimeraseの間違いと思われる。)

S.lividansでS.avermitilis avrBCDEFGHIを発現させて外因性aglyconeと反応させて産物解析。
delta-avrFな発現株では、C-13にD-olivoseが1つbeta-glycoside結合したavermectin A1a3を産生する。
avermectin A1a3を産生できるのはdelta-avrFのみ。
C-3での立体化学は最終的に3-O-methyltransferaseによって決定されるので、AvrF proteinは5-epimeraseであって3,5-epimeraseではない。

close this sectionSequence

selected fasta
>putative NDP-hexose epimerase [putative 3-5-epimerase]
MQTRSLKVAGVLEFSPPVFRDPRGLFVSPFQRDAFVAATGHELFGVAQTSFSVSRRGAVR
GIHYTRVPPGTAKYVHCPRGRALDMVVDLRLGSPTFGAWDSVVLDQDDCRALYLPVGVGH
AFAALTDDTLMAYTLSTAYAPENELAVSVHDPRLRLPLPRDVPPLLSERDRAAPTLAEAA
ARGLLPDYTRCREAEQVLAGAPVPSAGSAGEPGGGPAASPATDATP
selected fasta
>putative NDP-hexose epimerase [putative 3-5-epimerase]
ATGCAGACACGCTCATTGAAGGTGGCCGGAGTCCTGGAGTTCAGCCCGCCCGTGTTCCGC
GATCCACGCGGCCTGTTCGTCTCGCCCTTCCAGCGGGACGCCTTCGTGGCGGCGACCGGG
CACGAGCTGTTCGGCGTCGCGCAGACGAGCTTCAGCGTCTCCCGGCGCGGCGCCGTGCGC
GGCATCCACTACACCCGCGTGCCCCCGGGCACCGCCAAGTACGTCCACTGCCCGCGCGGC
CGGGCGCTCGACATGGTGGTGGACCTGCGGCTCGGATCACCCACCTTCGGCGCCTGGGAC
AGCGTGGTGCTCGACCAGGACGACTGCCGGGCCCTCTACCTGCCGGTGGGAGTGGGACAC
GCCTTCGCCGCACTGACCGACGACACGCTCATGGCGTACACGCTCTCCACCGCGTACGCC
CCGGAGAACGAACTCGCCGTCTCGGTGCACGATCCGCGGCTGCGGCTCCCGCTGCCCCGT
GACGTCCCGCCACTGCTCTCCGAGCGGGACCGCGCGGCTCCCACGCTCGCCGAGGCCGCG
GCACGGGGCCTGCTGCCCGACTACACCCGGTGCCGCGAGGCCGAGCAGGTCCTGGCCGGC
GCACCGGTCCCATCCGCCGGGAGCGCCGGGGAGCCCGGGGGAGGCCCCGCTGCTTCGCCC
GCCACCGACGCCACGCCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000888 dTDP-4-dehydrorhamnose 3,5-epimerase-related (Family)
[1-171] PD001462
PD001462   dTDP_sugar_isom
[4-178] 1.80000000000002e-51 PF00908
PF00908   dTDP_sugar_isom
IPR011051 RmlC-like cupin domain (Domain)
[1-183] 1e-65 SSF51182
SSF51182   RmlC_like_cupin
IPR014710 RmlC-like jelly roll fold (Domain)
[1-191] 2.99999999999998e-56 G3DSA:2.60.120.10
G3DSA:2.60.120.10   RmlC-like_jellyroll
SignalP
[1-36] 0.166 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Heda_00180 Heda_00180   Heda_00200 Heda_00200
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