Heda_00190 Heda_00190   Heda_00210 Heda_00210

Heda_00200 : CDS information

close this sectionLocation

Organism
StrainATCC 15422
Entry nameHedamycin
Contig
Start / Stop / Direction29,467 / 30,606 / + [in whole cluster]
29,467 / 30,606 / + [in contig]
Location29467..30606 [in whole cluster]
29467..30606 [in contig]
TypeCDS
Length1,140 bp (379 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative C-glycosyltransferase
Product (GenBank)glycosyltransferase
GenehedJ
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • orf13
Reference
ACC
PmId
[15271354] The hedamycin locus implicates a novel aromatic PKS priming mechanism. (Chem Biol. , 2004)
comment
hedamycinの生合成gene clusterの報告。

hedJ: Glycosyltransferase

配列解析のみ。Figure 2だとORFの向きが逆。
C-glycosyltransferasesの候補。
medermycin生合成でangolosamine移動を担うC-glycosyltransferaseであるMed-Orf8にとても似ている。

HedJ and HedLには、主にO-glycosyltransferasesに制限されているputative general base Asp残基が保存されていない。
普通はAla(A), Val(V), and Ile(I)のような非極性残基で置換されるが、HedJとGra-Orf14はLys(K)で置換されている。

C-glycosyltransferメカニズムについて検討あり。
Related Reference
ACC
Q70J67
NITE
Chro_00310
PmId
[16391039] Deoxysugar transfer during chromomycin A3 biosynthesis in Streptomyces griseus subsp. griseus: new derivatives with antitumor activity. (Appl Environ Microbiol. , 2006)
comment
Blast 3rd, 45%, 7e-60
Streptomyces griseus subsp. griseus_cmmGII
Glycosyltransferase
[Chro_00310]D-oliose glycosyltransferase

chromomycin gene clusterにある4つのGTs gene (cmmGI, cmmGII, cmmGIII, and cmmGIV)の不活化mutant作製、産物解析。

cmmGII mutantはposition 8でのdisaccharide鎖を欠いた4環化合物産生。
ammGI mutantはposition 8でのdisaccharide鎖の2つめの糖を欠いた3環化合物を産生。

よって、生合成中間体prechromomycin A3の8 positionへCmmGIIが1つめ、CmmGIが2つめのD-oliosyl基を取り込むことによりdisaccharideの形成を担う。
ACC
Q9ZGC0
NITE
Lando_00200
PmId
[15977274] LanGT2 Catalyzes the First Glycosylation Step during landomycin A biosynthesis. (Chembiochem. , 2005)
[21513888] Rational design of an aryl-C-glycoside catalyst from a natural product O-glycosyltransferase. (Chem Biol. , 2011)
comment
Blast 9th, id38%, 2e-42
Streptomyces cyanogenus_lanGT2
Glycosyl transferase homolog
[Lando_00200]D-olivose glycosyltransferase

---
[PMID: 15977274](2005) abstract
lanGT2不活化mutantの産物は糖側鎖を欠損。
よってLanGT2がhexasaccharide生合成においてpriming glycosyl transfer(polyketide骨格のO-8へのD-olivoseの付着)を触媒することを示す。

lanGT2 mutantでurdGT2を発現すると新規C-glycosylated angucyclineが産生されるのを確認している。
よってLanGT2とUrdGT2は似ているが異なる機能を持つ。

---
[PMID: 21513888](2011)
O-glycosyltransferase LanGT2を操作して、UrdGT2のようなC-Glycosylation活性を持つようにしている。
LanGT2, UrdGT2の反応メカニズムを提唱。
ACC
Q9RPA7
NITE
Urd_00200
PmId
[10658661] Two new tailoring enzymes, a glycosyltransferase and an oxygenase, involved in biosynthesis of the angucycline antibiotic urdamycin A in Streptomyces fradiae Tu2717. (Microbiology. , 2000)
[17640665] Structure and action of the C-C bond-forming glycosyltransferase UrdGT2 involved in the biosynthesis of the antibiotic urdamycin. (J Mol Biol. , 2007)
[12696864] The C-Glycosyltransferase UrdGT2 is unselective toward d- and l-configured nucleotide-bound rhodinoses. (J Am Chem Soc. , 2003)
comment
Blast 53rd, id33%, 1e-31
Streptomyces fradiae_urdGT2
Glycosyl transferase
[Urd_00200]C-glycosyltransferase

--
[PMID: 10658661](2000)
urdGT2が削除されたmutantは、urdamycin Aの代わりに、C-C connected deoxysugar moietyを欠いたtetracyclic angucyclinonesであるurdamycin I, urdamycin J and urdamycin Kを産生した。したがって、UrdGT2はNDP-D-olivoseのC-glycosyltransferを触媒する。

---
構造解析など続報あり。

close this sectionSequence

selected fasta
>putative C-glycosyltransferase [glycosyltransferase]
MKVLVNTGPAHPLFFPLVPLAWALHAAGHHVLVATPESFEPVVRGAGLRMAPVYGPLDMG
EVMGRDREGNRLRVPDSEEEMADGVGCGFARLAVRTLEPTVELVGSWRPDLVIGESYSFA
SAAIAAAVHGVPWVKHTVGPGDLPVTAALARELAPELKEHGLVEMPRPDLVLDNCPPLMG
GPAPDTTPVRYVPYGEPGVVPDWALRPGVRPRVLVTLGSVQPQIGGLPALAEMLAAVAEL
PVDLVVAVADHLVPGLGPLPANVIAAGWQSLTAVLPGCDLAVHHGGPGTMMACSFHGLPQ
VIVPGRGKPLDEIRRLAATGAVTEVPPAELTPHALRDACRTLLDDPGRRERARALRDHIA
EQPSPLRVVGVLEELAAAR
selected fasta
>putative C-glycosyltransferase [glycosyltransferase]
ATGAAGGTTCTGGTCAACACGGGTCCCGCCCACCCGCTGTTCTTCCCCCTGGTGCCGCTC
GCCTGGGCGCTGCACGCGGCCGGCCACCATGTGCTGGTCGCGACCCCGGAGAGCTTCGAG
CCGGTGGTGCGGGGCGCGGGCCTGCGCATGGCCCCGGTGTACGGACCGCTCGACATGGGT
GAGGTCATGGGCCGCGACCGCGAGGGCAACCGGCTGCGGGTCCCGGACTCCGAGGAGGAG
ATGGCCGACGGCGTGGGCTGCGGCTTCGCCCGCCTGGCCGTCCGGACGCTGGAGCCCACC
GTGGAACTGGTCGGTTCCTGGCGGCCCGACCTGGTGATCGGCGAGTCCTACTCGTTCGCC
TCCGCCGCGATCGCCGCCGCCGTCCACGGTGTGCCCTGGGTCAAGCACACGGTCGGACCC
GGCGACCTGCCGGTCACCGCCGCCCTGGCCCGCGAACTCGCTCCCGAGCTCAAGGAGCAC
GGGCTGGTGGAAATGCCGCGCCCCGACCTCGTGCTGGACAACTGCCCGCCCCTGATGGGC
GGCCCGGCCCCGGACACGACCCCCGTGCGGTACGTCCCCTACGGGGAACCCGGTGTGGTC
CCGGACTGGGCACTGCGCCCGGGCGTCCGGCCCCGGGTCCTGGTGACGCTGGGCTCGGTC
CAGCCGCAGATCGGCGGTCTGCCGGCGCTCGCCGAGATGCTGGCGGCGGTGGCGGAGCTG
CCCGTCGACCTCGTGGTCGCGGTCGCCGACCACCTCGTGCCCGGGCTGGGTCCGCTGCCC
GCCAACGTCATCGCCGCGGGCTGGCAGTCGCTCACCGCCGTCCTGCCCGGCTGCGACCTC
GCCGTGCACCACGGAGGGCCGGGCACCATGATGGCCTGTTCCTTCCACGGGCTGCCCCAA
GTGATCGTCCCCGGTCGCGGCAAGCCGCTGGACGAGATCCGGCGGCTGGCCGCGACCGGC
GCCGTGACCGAGGTTCCGCCCGCGGAGCTGACCCCGCACGCGCTGCGCGACGCCTGCCGG
ACCCTGCTCGACGACCCCGGCCGGCGCGAGCGGGCCCGGGCGCTCCGCGACCACATCGCC
GAGCAGCCGTCGCCCCTGCGCGTCGTCGGCGTGCTCGAGGAACTCGCGGCGGCCAGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002213 UDP-glucuronosyl/UDP-glucosyltransferase (Family)
[282-355] 2.9e-05 PF00201
PF00201   UDPGT
IPR004276 Glycosyl transferase, family 28 (Domain)
[14-138] 6.90000000000001e-10 PF03033
PF03033   Glyco_transf_28
IPR010610 Domain of unknown function DUF1205 (Domain)
[172-263] 4.70000000000001e-20 PF06722
PF06722   DUF1205
SignalP
[1-28] 0.982 Signal
Eukaryota   
[1-23] 0.476 Signal
Bacteria, Gram-positive   
[1-26] 0.405 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Heda_00190 Heda_00190   Heda_00210 Heda_00210
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