Heda_00210 : CDS information

Location

Organism	Streptomyces griseoruber
Strain	ATCC 15422
Entry name	Hedamycin
Contig	AY196994
Start / Stop / Direction	30,644 / 31,621 / + [in whole cluster] 30,644 / 31,621 / + [in contig]
Location	30644..31621 [in whole cluster] 30644..31621 [in contig]
Type	CDS
Length	978 bp (325 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose C-4 ketoreductase
Product (GenBank)	probable dTDP-4-keto-6-deoxyhexose reductase
Gene	hedI
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	orf12
Reference	ACC Q67G37 PmId [15271354] The hedamycin locus implicates a novel aromatic PKS priming mechanism. (Chem Biol. , 2004) comment hedamycinの生合成gene clusterの報告。 hedI: 4,6-Hexoseketoreductase 配列解析のみ。Figure 2だとORFの向きが逆。 alpha-L-N,N-dimethylvancosamine (3-dimethylamino-2,3,6-trideoxy-3-C-methyl-alpha-L-lyxo-hexopyranose)の生合成での4-KRに割り当てられている。参照しているのはvancomycin gene clusterでのdTDP-L-vancosamine生合成経路。
Related Reference	ACC O33708 NITE Adria_00200 PmId [9209071] Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine. (J Bacteriol. , 1997) [10631513] A two-plasmid system for the glycosylation of polyketide antibiotics: bioconversion of epsilon-rhodomycinone to rhodomycin D. (Chem Biol. , 1999) [9447597] Production of the antitumor drug epirubicin (4'-epidoxorubicin) and its precursor by a genetically engineered strain of Streptomyces peucetius. (Nat Biotechnol. , 1998) comment Blast 5th, id47%, 5e-58 Streptomyces peucetius_dnmV thymidine diphospho-4-keto-2,3,6-trideoxyhexulose reductase [Adria_00200]NDP-hexose C-4 ketoreductase --- [PMID: 9209071](1997) dnmV: 4-keto基の還元 dnmV不活化mutant産物解析と相補による回復から、daunosamine生合成に関与することは確認されている。dTDP-4-ketodeoxyhexulose ketoreductase機能の割り当ては配列解析に基づく。 --- [PMID: 10631513](1999) 異種性ホストにおけるL-daunosamineの生合成と付着のための最少の情報は、dnmLMJVUTS genesによってコードされる。 DnmVとavrE and eryBIV genesの産物は、DNR and DXRに由来する4'-epimeric anthracyclinesの合成を通して、dTDP-4-ketohexulose reductasesであることが証明されている(ref26)。 ↓そのref26 [PMID: 9447597](1998) abstract S.peucetius dnmV mutantに、Streptomyces avermitilis avrE or Saccharopolyspora eryBIV genesを異種性に導入すると、epirubicin (4'-epidoxorubicin) and 4'-epidaunorubicinを形成。 Saccharopolyspora eryBIV = dTDP-4-keto-6-deoxy-L-hexose 4-reductase

Sequence

>putative NDP-hexose C-4 ketoreductase [probable dTDP-4-keto-6-deoxyhexose reductase]
MTRRQAAGAGRVAVLGGTGWVGRHVCRAFSRAGHEVLVVARRPAEHRAPHPFVALDLADA
PAGELRRLLADAGAGVVVNATDAANAADGWAVGEQALAALNVDLVERLVTAAGALPWRTR
LVHLGSILEYGETPHGTRVDERYPPRPTTAYTRSKLAGSTAVLDAARAGTVDATVLRLTN
VCGPHPSPQSLPGKLVELLAEAVRSGGPMIVGVTEARRDFVDVRDVAEAVLRAAGPGATG
RAVNIGSGRAVGIRELVRLFVTVAGSDPGILREERRPNTSLGGTWTCADIRLAGELLGWR
PRTGLAASLRDMWRTAARTPDGGRG

>putative NDP-hexose C-4 ketoreductase [probable dTDP-4-keto-6-deoxyhexose reductase]
GTGACACGACGGCAGGCGGCCGGAGCGGGGCGGGTGGCCGTCCTCGGCGGCACCGGCTGG
GTGGGCCGGCACGTGTGCCGGGCGTTCTCCCGCGCCGGTCACGAAGTCCTCGTGGTGGCT
CGCCGCCCCGCCGAGCACCGCGCCCCGCATCCCTTCGTCGCGCTCGACCTCGCCGACGCC
CCGGCCGGCGAGCTGCGCCGGCTGCTGGCCGACGCGGGCGCGGGCGTGGTGGTCAACGCG
ACCGACGCAGCCAACGCCGCCGACGGCTGGGCCGTCGGCGAGCAGGCGCTGGCCGCGCTC
AACGTGGACCTGGTGGAACGGCTCGTCACGGCGGCCGGCGCGCTGCCGTGGCGCACCCGG
CTGGTCCACCTGGGCTCGATCCTCGAGTACGGCGAGACGCCGCACGGCACCCGGGTGGAC
GAGCGCTACCCGCCCCGGCCGACGACCGCCTACACCCGCAGCAAGCTGGCCGGTTCCACG
GCCGTGCTGGACGCGGCGCGCGCCGGCACGGTCGACGCGACGGTGCTGCGCCTGACGAAC
GTCTGCGGCCCCCACCCCTCCCCGCAGAGCCTGCCGGGCAAGCTGGTCGAGCTGCTCGCC
GAGGCGGTGCGCTCGGGCGGTCCCATGATCGTGGGCGTGACCGAGGCCCGACGCGACTTC
GTCGATGTCCGCGACGTCGCGGAGGCGGTGCTCCGCGCCGCCGGGCCGGGGGCGACCGGC
CGCGCCGTCAACATCGGCAGCGGCCGGGCCGTCGGCATCCGCGAGCTGGTACGGCTCTTC
GTCACGGTCGCGGGGAGCGACCCGGGCATCCTGCGCGAGGAACGGCGGCCCAACACCAGC
CTGGGCGGGACCTGGACCTGCGCGGACATCCGGCTCGCCGGTGAACTGCTCGGCTGGCGC
CCGCGCACCGGCCTGGCCGCCTCACTGCGCGACATGTGGCGGACCGCCGCCCGGACACCG
GACGGCGGCCGAGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	Eukaryota
TMHMM	No significant hit

Heda_00200 Heda_00220