Heda_00210 Heda_00210   Heda_00230 Heda_00230

Heda_00220 : CDS information

close this sectionLocation

Organism
StrainATCC 15422
Entry nameHedamycin
Contig
Start / Stop / Direction32,368 / 31,640 / - [in whole cluster]
32,368 / 31,640 / - [in contig]
Locationcomplement(31640..32368) [in whole cluster]
complement(31640..32368) [in contig]
TypeCDS
Length729 bp (242 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose N,N-dimethyltransferase
Product (GenBank)N,N-dimethyltransferase
GenehedH
Gene (GenBank)
EC number2.1.1.-
Keyword
Note
Note (GenBank)
  • orf11
Reference
ACC
PmId
[15271354] The hedamycin locus implicates a novel aromatic PKS priming mechanism. (Chem Biol. , 2004)
comment
hedamycinの生合成gene clusterの報告。

hedH: N,N-di-Methyltransferase

配列解析のみ。Figure 2だとORFの向きが逆。
beta-D-angolosamine (3-dimethylamino-2,3,6-trideoxy-beta-D-arabino-hexopyranose)の生合成経路に割り当てられている。
参照しているのはvancomycin gene clusterでのdTDP-L-vancosamine生合成経路。
Related Reference
ACC
Q9ZGH6
NITE
Pikro_00100
PmId
[9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998)
[12119032] Expression, purification, and characterization of two N,N-dimethyltransferases, tylM1 and desVI, involved in the biosynthesis of mycaminose and desosamine. (Biochemistry. , 2002)
comment
Blast 3rd, id54%, 1e-67
Streptomyces venezuelae_desVI
N,N-dimethyltransferase

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[PMID: 9770448](1998)
12員環骨格を持つMethymycin and Neomethymycinと、14員環骨格を持つNarbomycin and Pikromycinの生合成で共有されるclusterの同定。

DesVI: N,N-Dimethyltransferase

desI, desII, desIII, desIV, desV, desVI, and desVIII はdeoxysugar生合成を担う。

desVI のDeletion/replacement mutationにより、methymycin, neomethymycin, narbomycin, and pikromycinは非産生。それらのaglyconeである10-deoxymethynolide and narbonolideを蓄積。

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[PMID: 12119032](2002)abstract
N,N-dimethyltransferasesであるtylM1 and desVI の過剰発現、精製、触媒機能確認。
TylM1 and DesVI はhomodimeric proteinで、ほとんど同じ生化学的特性を持つ。

close this sectionSequence

selected fasta
>putative NDP-hexose N,N-dimethyltransferase [N,N-dimethyltransferase]
MYGTEFAEIYDLVYAARGKDYAGECAEVVRHVRARRPDAASLLDVACGTGAHLALLRDEF
AVVEGLELSEHMIARARAGLPDVPVHQGDMRGFRTGRAYDALTCMFSSIGYVGSPEDLGR
ALSSMARHLTPGGVLVLEPWYFPEAFLPEYIADDLVRTGDRVVARVSYSAREGDRVPIVV
HYVDALKGRGIRHFTDVHRMSLFTREQYRTAFERAGCSVEYVEGGPFRCGLFVGVREDGP
PA
selected fasta
>putative NDP-hexose N,N-dimethyltransferase [N,N-dimethyltransferase]
GTGTACGGAACGGAGTTCGCCGAGATCTACGACCTGGTCTACGCCGCGCGCGGCAAGGAC
TACGCCGGCGAGTGCGCGGAGGTGGTGCGGCATGTGCGGGCACGCCGCCCGGACGCCGCC
TCCCTGCTGGACGTGGCGTGCGGAACGGGTGCCCATCTGGCCCTGCTGCGGGACGAGTTC
GCGGTGGTGGAGGGGCTCGAACTGTCCGAGCACATGATCGCCCGGGCGCGGGCCGGCCTG
CCGGACGTGCCGGTCCACCAGGGCGACATGCGCGGCTTCCGCACCGGTCGCGCGTACGAC
GCGCTCACGTGCATGTTCAGCTCCATCGGGTACGTCGGCTCGCCGGAGGACCTCGGCCGG
GCGCTGAGCAGCATGGCGCGGCATCTGACGCCCGGCGGGGTGCTGGTCCTCGAACCCTGG
TACTTCCCCGAGGCGTTCCTGCCCGAGTACATCGCGGACGACCTGGTCCGGACGGGGGAC
CGGGTCGTGGCGCGGGTGTCGTACTCGGCGCGGGAGGGCGACCGGGTGCCGATCGTGGTG
CACTACGTCGACGCCCTCAAGGGCCGCGGCATCCGGCACTTCACGGACGTGCACCGCATG
AGCCTGTTCACGCGGGAGCAGTACCGGACCGCCTTCGAGCGGGCGGGCTGCTCCGTGGAG
TACGTCGAGGGCGGCCCGTTCCGCTGCGGTCTCTTCGTCGGCGTGCGCGAAGACGGGCCG
CCGGCGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP
[1-34] 0.041 Signal
Bacteria, Gram-negative   
[1-15] 0.074 Signal
Eukaryota   
TMHMM No significant hit
Heda_00210 Heda_00210   Heda_00230 Heda_00230
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