Heda_00240 : CDS information

Location

Organism	Streptomyces griseoruber
Strain	ATCC 15422
Entry name	Hedamycin
Contig	AY196994
Start / Stop / Direction	35,231 / 33,606 / - [in whole cluster] 35,231 / 33,606 / - [in contig]
Location	complement(33606..35231) [in whole cluster] complement(33606..35231) [in contig]
Type	CDS
Length	1,626 bp (541 aa)

Annotation

Category	5.1 general function
Product	putative hydrolase
Product (GenBank)	putative secreted tripeptidylaminopeptidase
Gene	orfH3
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	orf9
Reference	ACC Q67G34 PmId [15271354] The hedamycin locus implicates a novel aromatic PKS priming mechanism. (Chem Biol. , 2004) comment hedamycinの生合成gene clusterの報告。 orfH3: Transaminopeptidase 配列解析のみ。 S. lividansのtripeptidyl aminopeptidase (TAP)に強い相同性あり。
Related Reference	ACC Q54410 PmId [7487044] ( , ) comment Blast 38th, id32%, 3e-42 Streptomyces lividans_tap Tripeptidyl aminopeptidase(EC 3.4.14.-) Tripeptidylaminopeptidase precursor tripeptidyl aminopeptidase (Tap) geneを抽出、過剰発現させた結果、産物は分子量55000の分泌タンパク質で、主要なN末分解活性を有していた。また、相同性やlipases、 esterases、acyl transferases等を考慮し、active siteはputative serine残基であるといっている。(このserine残基はorfH3で保存されていない。) primer extensionで転写開始点の同定も合わせて行なっている。 Tap活性を欠いたmutant株でも普通に生育し、胞子形成することができる。

Sequence

>putative hydrolase [putative secreted tripeptidylaminopeptidase]
MAPSLAGRRRGTPEDGWSAGVESCSTPVRGPGGSLAAMTRDAQPPSSAAFHTQRPTWAPA
PSGDGVEHATVQVPLDYADPAGATLTIAVSRRRATDPARRRGVLLALNGGPGGYFGLGRR
FAQVLAATPLAEVYDLIGFDPRGTGDSTPLHAEITPRRTPVDSRPPESAFPLLAEDARDR
EEGNRRGGGARRPHFSTRNVARDMDVLRAVLGEPVINYLGYTYGTYAGAVYGAMFPERLD
RSVLDSCVHPDWTWREQLMAQGAAHRANVDAWAQWTARRRGHFGLGSSAAEVLDAVERTV
AHVETLPDGVRLRTLLDSALGSRSADRARWSELGRMVGSLAGADGPTAAALLADERIWPP
EETEGATRCGVLDAVILEKDWPDDLETYYADMRRFRARHPYGCGVTRAQPLPGAFRTFTP
PEPPTRLVRDGYPAGLVVHADGDPVDHYPGGVALARRLGHRLVTVADSGQHEIYAFQRNK
QVDEVVEAYLVDGVLPEGDVTCPSTVARPDLPEDDAGGHPDGVGVNALAASADSDREGRH
A

>putative hydrolase [putative secreted tripeptidylaminopeptidase]
GTGGCGCCCAGCCTGGCGGGGAGGCGTCGAGGCACGCCTGAGGACGGTTGGAGCGCCGGG
GTCGAGTCCTGCTCCACGCCTGTTCGAGGCCCAGGCGGCAGTCTCGCCGCCATGACCCGT
GACGCTCAGCCGCCGTCGTCGGCGGCGTTTCACACGCAGCGCCCCACGTGGGCCCCCGCG
CCGTCGGGCGACGGGGTGGAACACGCCACCGTGCAGGTGCCACTCGACTACGCGGACCCC
GCGGGCGCCACCCTGACGATCGCCGTCAGCAGACGCAGGGCCACCGATCCCGCCCGCCGC
CGCGGCGTCCTGCTGGCACTGAACGGCGGGCCGGGCGGCTACTTCGGGCTCGGCCGACGC
TTTGCCCAGGTGCTGGCGGCCACGCCTCTCGCGGAGGTGTACGACCTGATCGGTTTCGAC
CCGCGCGGCACCGGCGACTCCACTCCGCTGCACGCGGAGATCACCCCCCGCCGCACGCCC
GTGGACTCGCGTCCGCCGGAGTCCGCCTTCCCCCTGCTCGCCGAGGACGCGCGCGACCGG
GAGGAGGGGAACCGGCGCGGCGGCGGGGCGCGCCGGCCGCACTTCAGCACCCGCAACGTC
GCCCGGGACATGGACGTCCTGCGCGCGGTGCTCGGCGAGCCCGTCATCAACTACCTGGGC
TACACGTACGGGACGTACGCGGGCGCCGTCTACGGCGCGATGTTCCCCGAGAGGCTGGAC
CGCAGCGTCCTCGACTCCTGTGTGCACCCGGACTGGACCTGGCGCGAGCAACTGATGGCC
CAGGGCGCGGCACACCGGGCGAACGTCGACGCGTGGGCTCAGTGGACGGCCCGACGGCGC
GGGCACTTCGGGCTCGGCTCGTCGGCCGCGGAGGTGCTGGACGCCGTGGAACGGACCGTG
GCGCACGTCGAGACGCTGCCCGACGGGGTGCGGCTGCGCACCCTGCTGGACTCGGCGCTC
GGCTCGCGCTCCGCGGACCGGGCGCGCTGGTCTGAGCTGGGCCGGATGGTGGGTTCGCTG
GCCGGCGCGGACGGTCCCACCGCCGCGGCGCTGCTGGCGGACGAGCGGATCTGGCCGCCG
GAGGAGACGGAGGGCGCGACACGCTGCGGGGTTCTGGACGCCGTCATCCTGGAGAAGGAC
TGGCCGGACGACCTCGAGACGTACTACGCCGACATGCGCCGCTTCCGCGCCCGTCACCCT
TACGGGTGCGGGGTGACGCGGGCCCAGCCGCTGCCCGGCGCGTTCCGCACCTTCACCCCG
CCGGAGCCGCCCACCCGGCTGGTCCGGGACGGCTATCCGGCCGGGCTGGTCGTGCACGCC
GACGGCGACCCCGTCGACCACTACCCGGGCGGCGTGGCGCTGGCCCGGCGACTGGGCCAC
CGGCTGGTGACGGTCGCCGACTCCGGCCAGCACGAGATCTACGCCTTCCAGCGCAACAAG
CAGGTGGACGAGGTCGTCGAGGCGTACCTGGTCGACGGAGTGCTGCCGGAGGGCGACGTC
ACCTGCCCGAGCACCGTTGCCCGCCCGGATCTGCCCGAGGACGACGCCGGCGGGCACCCG
GACGGCGTCGGGGTGAACGCCCTCGCCGCCTCGGCCGACAGCGACCGCGAAGGAAGGCAC
GCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR013595 Peptidase S33 tripeptidyl aminopeptidase-like, C-terminal (Domain) PF08386 Abhydrolase_4
SignalP	No significant hit
TMHMM	No significant hit

Heda_00230 Heda_00250