Indano_00030 : CDS information

Location

Organism	Streptomyces antibioticus
Strain	NRRL 8167
Entry name	Indanomycin
Contig	FJ545274
Start / Stop / Direction	3,240 / 1,951 / - [in whole cluster] 3,240 / 1,951 / - [in contig]
Location	complement(1951..3240) [in whole cluster] complement(1951..3240) [in contig]
Type	CDS
Length	1,290 bp (429 aa)

Annotation

Category	5.1 general function
Product	putative penicillin-binding protein
Product (GenBank)	putative penicillin binding protein
Gene
Gene (GenBank)
EC number
Keyword
Note	This ORF is not involved in indanomycin biosynthesis.
Note (GenBank)	Orf3
Reference	ACC C5HV01 PmId [19301315] Analysis of the indanomycin biosynthetic gene cluster from Streptomyces antibioticus NRRL 8167. (Chembiochem. , 2009) comment indanomycin biosynthetic gene clusterに関する文献。 orf3(429a.a): putative penicillin-binding protein orf3自体の機能実験は無いが、欠損させてもindanomycinの生産が減少したり消失することは無いとの記載有り。
Related Reference	ACC Q9L656 PmId [11094280] Cloning and characterization of the gene encoding penicillin-binding protein A of Streptomyces griseus. (FEMS Microbiol Lett. , 2000) comment Blast 21st, id38%, 4e-64 Streptomyces griseus PbpA Streptomyces griseus_pbpAのクローニング、特徴づけ。 pbpA mutantsにおいて、growth, sporulation, or spore sonication resistanceに影響なし。よってS. griseusの一般条件における生育や胞子形成において、PBPAは必須ではないと示唆される。
Family/Domain	FD IPR001460 comment [IPR001460] Penicillin-binding protein, transpeptidase (Domain) Function GO:0008658 penicillin binding

Sequence

>putative penicillin-binding protein [putative penicillin binding protein]
MTSGCSQDTHTGTGDGSPAEHASPSASRRGLGDILVGGKAVTGSQPTNLPKAPYQRTYTD
GPLYAPVTGYRSMAYGRAGLEALYDDSLSAGAQGDVATTIEPAVQKAAFDALGDRQGAAV
ALDAESGRLLTLVSTPSYDPGQFSGRGVKDAQAWRSATADSRSPMLNRALKQADAPGATF
SVVVAAAALDEGLYATVDDATVSPLTYVLPLSTTRVTGASSQCENASIGLALHRSCANVF
TKLAAELGQDRLRAAARKFGFDDDTLKVPLTVAESSYPGDGTTAPEAALTGMGAGDVRAT
PLQMARVAAAVANGGRLVSPRLVEGVTKADGTTQLPEAADAGSAGQAMSRGTAEALRAAL
GSGGQAGWVPSGDGDLAASWFIGSAPSGDGRRVAVAVRVEGLPSTAGAEESAGGRAAAEA
AEKILASVS

>putative penicillin-binding protein [putative penicillin binding protein]
GTGACCAGCGGCTGCAGCCAGGACACGCACACCGGCACCGGGGACGGGTCCCCGGCGGAG
CACGCCTCCCCGTCCGCGTCCAGACGCGGACTGGGCGACATTCTCGTCGGCGGGAAAGCC
GTCACCGGCTCGCAGCCCACGAACCTCCCGAAGGCCCCCTACCAGCGCACCTATACCGAC
GGTCCCCTCTATGCGCCCGTCACGGGCTACCGGTCGATGGCTTACGGCCGCGCGGGCCTC
GAGGCCCTGTACGACGACTCGCTGAGCGCCGGGGCGCAGGGTGACGTCGCGACGACCATC
GAACCCGCCGTCCAGAAAGCCGCGTTCGACGCGCTGGGTGACCGGCAGGGCGCCGCGGTG
GCTCTCGACGCGGAGTCCGGGAGGCTTCTCACCCTGGTGAGTACGCCTTCCTACGACCCG
GGGCAGTTCAGCGGCCGCGGAGTGAAGGACGCCCAGGCGTGGAGGTCGGCCACCGCCGAC
AGCCGATCGCCGATGCTCAACCGGGCGCTGAAACAGGCCGATGCGCCGGGCGCGACCTTC
AGCGTCGTCGTCGCGGCCGCCGCCCTGGACGAAGGCCTCTACGCGACGGTGGACGACGCC
ACCGTCAGCCCGCTCACCTATGTGCTGCCCCTCTCGACGACGCGCGTGACCGGCGCGTCG
TCGCAGTGCGAGAACGCCTCCATCGGACTGGCGTTGCACCGCTCCTGCGCCAACGTGTTC
ACCAAGCTGGCCGCCGAGCTCGGTCAGGACCGACTCCGCGCCGCGGCACGGAAGTTCGGC
TTCGACGACGACACGCTCAAGGTGCCCTTGACGGTCGCCGAGAGCAGCTACCCCGGTGAC
GGCACGACGGCACCCGAAGCGGCCCTCACGGGGATGGGAGCCGGGGACGTCCGGGCAACT
CCGCTGCAGATGGCGCGAGTCGCGGCCGCGGTCGCCAACGGCGGGCGGCTGGTGTCCCCC
CGGCTGGTGGAAGGGGTGACGAAGGCCGACGGCACGACGCAGCTGCCCGAGGCCGCCGAC
GCTGGTTCTGCCGGGCAGGCGATGTCCCGGGGCACGGCTGAGGCGTTGCGGGCGGCACTC
GGTTCCGGCGGACAGGCGGGGTGGGTCCCCAGCGGTGACGGCGACCTGGCCGCCTCGTGG
TTCATCGGCTCCGCGCCGTCGGGCGACGGACGGCGCGTTGCCGTGGCGGTCCGGGTCGAG
GGCCTGCCGTCGACGGCCGGCGCCGAGGAGTCCGCCGGCGGACGGGCAGCGGCCGAGGCG
GCGGAGAAGATTCTCGCGAGCGTCTCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001460 Penicillin-binding protein, transpeptidase (Domain) PF00905 Transpeptidase IPR012338 Beta-lactamase/transpeptidase-like (Domain) SSF56601 PBP_transp_fold G3DSA:3.40.710.10 G3DSA:3.40.710.10
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Indano_00020 Indano_00040