Indano_00130 Indano_00130   Indano_00150 Indano_00150

Indano_00140 : CDS information

close this sectionLocation

Organism
StrainNRRL 8167
Entry nameIndanomycin
Contig
Start / Stop / Direction17,886 / 19,376 / + [in whole cluster]
17,886 / 19,376 / + [in contig]
Location17886..19376 [in whole cluster]
17886..19376 [in contig]
TypeCDS
Length1,491 bp (496 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.4 Other
Productputative L-proline--PCP ligase
putative L-prolyl-PCP synthetase
Product (GenBank)proline adenyltransferase
Gene
Gene (GenBank)idmJ
EC number
Keyword
  • pyrrole ring moiety
Note
Note (GenBank)
Reference
ACC
PmId
[19301315] Analysis of the indanomycin biosynthetic gene cluster from Streptomyces antibioticus NRRL 8167. (Chembiochem. , 2009)
comment
indanomycin biosynthetic gene clusterに関する文献。

idmJ(496a.a): proline adenyltransferase

idmJの欠損株を作成し、indanomycin生産が消失していること、及びidmJを欠損株に相補することによって、indanomycin生産能が回復することを確認している。idmJ自体の機能実験は行っていないが、相同性よりproline adenyltransferaseと予想している模様。
Related Reference
ACC
Q9X3R4
NITE
Pyolu_00090
PmId
[11880032] Conversion of L-proline to pyrrolyl-2-carboxyl-S-PCP during undecylprodigiosin and pyoluteorin biosynthesis. (Chem Biol. , 2002)
comment
<Putative acyl-CoA synthetase(pltF), Pseudomonas fluorescens, BLAST 15, id=49%>

pyrrole biosynthesisについて新規のメカニズムを提言した文献。
pltF遺伝子をE. coliで発現させて精製し、ATP-[32P]PPi exchange活性測定を行い、基質としてL-proline特異的であることを確認している。また、holo-PCP(PltL)がPltFによってaminoacylateされることも確認している。

close this sectionPKS/NRPS Module

0
A28..417

close this sectionSequence

selected fasta
>putative L-proline--PCP ligase [proline adenyltransferase]
MNLHQLLVDTAAKEPDRLAVAGTAARLTYAELDSTANALAHRLRALGVGPGDRVVLWSDK
SPAVVAAMQAVLRLGAAYVPADGALPIARVAAMADDCSAAALLAPADRLAPVADVLGPRC
PAADLAQRPDPAAEPLNALVAPDDLAYILYTSGSTGAPKGVCISHRNARAFVDWAVEELA
PGPQDRFSNHAPFTFDLSVLDLYAAFSAGASVHLIPSELAYAPEQLVEFLHDRQITVWYS
VPSALTLMMRDGGLLDRPAPRALRTVLFAGEPFPLPGVRALAGWTDARLLNLYGPTETNV
CTRHEVRPTDLDGDRPLPIGTAVSGDRAWAEGPDGRLAAPGEEGELLVDGPTVMLGYWGG
EPHTGPYRTGDLVRPLPDGSFAYLGRRDHMVKVRGHRVELGEVESVLALHPDVAEAAAVV
VGSGMDGRLVAFVVPEPDRRPGVLSLVRHAAQRLPRYMVADEVRVLPGLPRTRNGKVDRL
ALRDTAEAPAPGAAAQ
selected fasta
>putative L-proline--PCP ligase [proline adenyltransferase]
GTGAACCTCCACCAGCTCCTCGTCGACACCGCCGCGAAGGAACCCGACCGGCTGGCCGTG
GCCGGAACCGCCGCCCGACTGACCTACGCGGAGCTCGACTCCACCGCCAACGCCCTCGCC
CACCGGCTGCGCGCCCTGGGCGTCGGGCCCGGTGACCGGGTCGTGCTGTGGTCGGACAAG
TCGCCGGCCGTCGTGGCCGCGATGCAGGCGGTGCTCCGCCTCGGCGCCGCCTACGTGCCC
GCCGACGGCGCCCTGCCGATCGCCCGGGTCGCCGCCATGGCCGACGACTGCTCGGCCGCC
GCGCTCCTGGCCCCGGCCGACCGTCTGGCGCCGGTCGCCGACGTGCTCGGCCCGCGGTGT
CCGGCCGCCGACCTCGCCCAGCGGCCCGACCCGGCCGCCGAACCCCTCAACGCGCTGGTG
GCGCCCGACGACCTGGCGTACATCCTCTACACCTCCGGATCGACCGGTGCTCCCAAAGGG
GTGTGCATCAGCCACCGCAACGCGCGCGCCTTCGTCGACTGGGCGGTCGAGGAGCTCGCC
CCCGGGCCGCAGGATCGCTTCTCCAACCATGCCCCGTTCACCTTCGACCTGTCCGTCCTG
GACCTGTACGCCGCGTTCTCGGCCGGCGCGTCGGTGCACCTGATTCCCTCCGAACTCGCC
TACGCACCGGAACAGTTGGTGGAGTTCCTGCACGACCGGCAGATCACGGTCTGGTACTCG
GTGCCGTCCGCCCTCACGCTGATGATGCGTGACGGGGGACTGTTGGACCGCCCCGCCCCC
CGAGCGCTGCGGACCGTGCTCTTCGCCGGGGAGCCCTTCCCCCTCCCGGGCGTCCGCGCG
CTGGCGGGTTGGACCGACGCCCGGCTGCTCAACCTCTACGGGCCCACCGAGACCAACGTC
TGTACCCGCCACGAGGTCCGCCCCACCGACCTCGACGGCGACCGGCCCCTGCCCATCGGC
ACCGCCGTCAGCGGCGACCGGGCCTGGGCCGAGGGCCCGGACGGGCGGCTCGCCGCACCC
GGCGAGGAGGGCGAACTGCTCGTCGACGGGCCGACGGTGATGCTCGGCTACTGGGGCGGG
GAACCGCACACCGGCCCCTACCGCACCGGCGACCTGGTCCGCCCGCTGCCCGACGGATCG
TTCGCCTACCTCGGCCGCCGCGATCACATGGTCAAAGTGCGCGGCCACCGGGTCGAACTG
GGCGAGGTCGAGTCCGTGCTCGCACTGCACCCGGACGTCGCGGAGGCGGCCGCCGTGGTC
GTGGGCAGCGGCATGGACGGCCGGCTGGTGGCGTTCGTGGTGCCCGAGCCGGACCGCCGT
CCCGGAGTGCTCTCGCTCGTACGGCACGCGGCGCAGCGGCTGCCGCGCTACATGGTCGCC
GACGAGGTGCGCGTACTGCCCGGGCTGCCCCGCACCCGCAACGGGAAGGTCGACCGCCTG
GCGCTGCGGGACACGGCCGAAGCCCCGGCCCCGGGCGCCGCCGCGCAGTGA
[0] A28..417
[0] A82..1251

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000873 AMP-dependent synthetase/ligase (Domain)
[28-417] 1.5e-97 PF00501
PF00501   AMP-binding
IPR010071 Amino acid adenylation domain (Domain)
[28-417] 1.99999999999999e-102 TIGR01733
TIGR01733   AA-adenyl-dom
IPR020459 AMP-binding (Domain)
[143-154] 2.79999889404114e-05 PR00154 [155-163] 2.79999889404114e-05 PR00154
PR00154   AMPBINDING
IPR020845 AMP-binding, conserved site (Conserved_site)
[148-159] PS00455
PS00455   AMP_BINDING
IPR025110 Domain of unknown function DUF4009 (Domain)
[457-487] 7.5e-06 PF13193
PF13193   DUF4009
SignalP No significant hit
TMHMM No significant hit
Indano_00130 Indano_00130   Indano_00150 Indano_00150
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