Indano_00130 : CDS information

Location

Organism	Streptomyces antibioticus
Strain	NRRL 8167
Entry name	Indanomycin
Contig	FJ545274
Start / Stop / Direction	16,756 / 17,889 / + [in whole cluster] 16,756 / 17,889 / + [in contig]
Location	16756..17889 [in whole cluster] 16756..17889 [in contig]
Type	CDS
Length	1,134 bp (377 aa)

Annotation

Category	2.2 modification addition of starter unit
Product	putative L-prolyl-PCP dehydrogenase
Product (GenBank)	prolyl carrier protein dehydrogenase
Gene
Gene (GenBank)	idmI
EC number
Keyword	pyrrole ring moiety
Note
Note (GenBank)
Reference	ACC C5HV11 PmId [19301315] Analysis of the indanomycin biosynthetic gene cluster from Streptomyces antibioticus NRRL 8167. (Chembiochem. , 2009) comment indanomycin biosynthetic gene clusterに関する文献。 idmI(377a.a): prolyl-S-dehydrogenase idmI自体の機能実験は無いが、相同性よりdehydrogenaseであるとしている。
Related Reference	ACC Q9X3R3 NITE Pyolu_00080 PmId [11880032] Conversion of L-proline to pyrrolyl-2-carboxyl-S-PCP during undecylprodigiosin and pyoluteorin biosynthesis. (Chem Biol. , 2002) comment <Putative acyl-CoA dehydrogenase(pltE), Pseudomonas fluorescens, BLAST 14, id=53%> pyrrole biosynthesisについて新規のメカニズムを提言した文献。 RedWとPltEは共にL-prolyl-S-PCP Dehydrogenasesとして機能することが示されている。 pltEをE.coliで過剰発現したPltEの精製はうまくいかなかった。そこでPltEの反応産物を加水分解処理してPCPから外し、HPLCで分離したところ、標準的なpyrrolyl-2-carboxylateに一致するピークを検出。1H-NMRでもこれが標準的なpyrrolyl-2-carboxylateに一致することを確認。HPLCで見られるもうひとつのピークは、加水分解処理による副産物と思われる。同時に検討されているRedWは精製が可能であり、・反応にL-proline、ORF11(PltFに相当)、holo-ORF9(PltLに相当)、ATPが必要なこと、・PltEと同じ産物を得ることが確かめられている。

Sequence

>putative L-prolyl-PCP dehydrogenase [prolyl carrier protein dehydrogenase]
MDFDLTEKQRQRYDDVLSAVRERLGEPPSGEPFTPARWQDAARIGLTGLCLPTEFGGGGL
GALDTALCLEAFGRGCPDTGLVFAVSAHLLACAVPIRDFADVSVRGELLSGLASGELVAA
NAMTEDDAGSDLSRLAVTAERKDDGYLLNGEKSFASNAPAADVLVTYGTSDPAAGFLGVT
AFVLRADLPGVQVGEPFRKMGLSGCPAGRVAFRDCHVPTSHRLGTEGQGSIIFQHSMGWE
RAILFAGYLGLMERQLEQCVRHARERRQFGHGIGEFQAVSHRVVGMKQRLEAARLLLYRA
CWLMDQGRDHSTAVALSKTAVSEGAVANSLDAIQIFGGSGYLSPAGIEQQLRDAVPSTIF
SGTTDIQREIVAREIGL

>putative L-prolyl-PCP dehydrogenase [prolyl carrier protein dehydrogenase]
GTGGATTTCGACCTCACCGAAAAACAGCGGCAACGCTATGACGACGTCTTGTCCGCAGTG
CGGGAGCGGCTCGGCGAGCCCCCCTCGGGCGAGCCGTTCACCCCTGCCCGATGGCAGGAC
GCGGCACGGATCGGCCTGACCGGCCTCTGCCTGCCGACCGAGTTCGGAGGCGGCGGTCTC
GGTGCGCTGGACACCGCGCTGTGCCTGGAGGCGTTCGGCCGGGGATGCCCCGACACCGGG
CTGGTGTTCGCCGTTTCCGCCCATCTGCTGGCCTGTGCCGTCCCGATCCGCGACTTCGCC
GACGTCTCGGTGCGCGGGGAGCTGCTGTCCGGGCTGGCGTCCGGAGAGCTCGTCGCCGCC
AATGCCATGACGGAGGACGACGCGGGTTCCGACCTGAGCCGTCTCGCCGTCACCGCGGAG
CGGAAGGACGACGGATACCTGCTGAACGGGGAGAAGTCGTTCGCCAGCAACGCCCCGGCC
GCCGATGTGCTCGTCACCTATGGCACGAGCGACCCCGCAGCGGGATTCCTCGGGGTCACG
GCCTTCGTGCTGCGCGCCGACCTGCCCGGTGTCCAAGTCGGTGAGCCCTTCCGCAAGATG
GGGCTCAGCGGCTGCCCCGCCGGCCGGGTCGCCTTCCGCGACTGCCACGTCCCCACCTCC
CACCGGCTCGGCACCGAGGGCCAGGGGAGCATCATCTTCCAGCACTCGATGGGCTGGGAA
CGCGCCATCCTGTTCGCCGGCTACCTCGGCCTGATGGAACGCCAGCTGGAGCAGTGCGTG
CGACATGCCCGCGAACGGCGCCAGTTCGGCCACGGCATCGGCGAGTTCCAGGCCGTCTCA
CACCGCGTCGTCGGCATGAAGCAGCGGCTGGAGGCCGCCCGGCTGCTGCTGTACCGCGCC
TGCTGGCTCATGGATCAGGGGCGTGACCACAGCACGGCGGTCGCCCTGTCCAAGACGGCC
GTCTCGGAGGGCGCGGTGGCCAACAGCCTCGACGCGATCCAGATCTTCGGGGGTTCCGGC
TATCTGAGCCCCGCCGGGATCGAACAGCAGCTCCGCGACGCCGTACCCAGCACGATCTTC
TCGGGGACCACGGACATTCAGCGCGAGATCGTCGCGAGGGAGATCGGCCTGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006090 Acyl-CoA oxidase/dehydrogenase, type 1 (Domain) PF00441 Acyl-CoA_dh_1 IPR006091 Acyl-CoA oxidase/dehydrogenase, central domain (Domain) G3DSA:2.40.110.10 Acyl_CoA_DH/ox_M PF02770 Acyl-CoA_dh_M IPR006092 Acyl-CoA dehydrogenase, N-terminal (Domain) PF02771 Acyl-CoA_dh_N IPR009075 Acyl-CoA dehydrogenase/oxidase C-terminal (Domain) G3DSA:1.20.140.10 AcylCoA_DH_1/2_C SSF47203 AcylCoADH_C_like IPR009100 Acyl-CoA dehydrogenase/oxidase (Domain) SSF56645 AcylCoA_dehyd_NM IPR013786 Acyl-CoA dehydrogenase/oxidase, N-terminal (Domain) G3DSA:1.10.540.10 AcylCoA_DH/ox_N
SignalP	No significant hit
TMHMM	No significant hit

Indano_00120 Indano_00140