Jado_00280 Jado_00280   Jado_00300 Jado_00300

Jado_00290 : CDS information

close this sectionLocation

Organism
StrainISP5230 (=NBRC 13096)
Entry nameJadomycin B
Contig
Start / Stop / Direction28,522 / 29,238 / + [in whole cluster]
10,429 / 11,145 / + [in contig]
Location28522..29238 [in whole cluster]
10429..11145 [in contig]
TypeCDS
Length717 bp (238 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productflavin reductase
Product (GenBank)JadY
Gene
Gene (GenBank)jadY
EC number
Keyword
Note
Note (GenBank)
  • contains an NAD(P)H dehydrogenase (quinone) domain; similar to Streptomyces coelicolor sequence deposited in GenBank Accession Number AL132184
Reference
ACC
PmId
[23177193] Identification of JadG as the B ring opening oxygenase catalyzing the oxidative C-C bond cleavage reaction in jadomycin biosynthesis. (Chem Biol. , 2012)
comment
UniProt登録されている[PMID:11932454]ではFigも含め、jadYについて記載なし。

--
[PMID:23177193](2012) abstract
jadomycin生合成におけるB環開環反応を担う酵素としてJadGを同定。
dehydrorabelomycin → jadomycin Aへの変換におけるcofactorとしてFMNH(2) or FADH(2)を必要とする。cofactorsは、clusterに位置しているflavin reductase JadYまたはEscherichia coli Freのどちらかにによって供給されうる。

JadYは、NAD(P)H-dependent FMN/FAD reductaseとして特徴づけられた。好ましい基質はFMNである。
jadY破壊mutantでもjadomycinが産生されたので、jadYの機能はホストで他の酵素によって置換されうる。
Related Reference
ACC
Q07923
PmId
[15184374] Crystal structure and functional characterization of yeast YLR011wp, an enzyme with NAD(P)H-FMN and ferric iron reductase activities. (J Biol Chem. , 2004)
comment
162nd, id38%, 8e-19, 末端イマイチ
Saccharomyces cerevisiae_LOT6
NAD(P)H-dependent FMN reductase LOT6(EC 1.5.1.29)

function; Has several reductase activities that are NAD(P)H-dependent and involve FMN as a cofactor, ferricyanide being the best substrate for reduction. May be involved in ferric iron assimilation.

close this sectionSequence

selected fasta
>flavin reductase [JadY]
MLSDESAKTGCPRNRRQVHRAIHRAKPGSEAEGAPHDPDRRHHRQHRPGRRSTAVAQWVE
EAAGRHEAIKAGGATVELVDIAEFGLPLLDESVPAAFGQYANEHTKRWSEKIASFDAFVF
VTPEYNRSVPAALKNAIDYLYAEWQNKSAGFVSYGVQGGTRAIDHLRVTLAEVKVADVPT
AVSLPVFTDFTFENPMDPTETGRVTPGPHQEPTLFKMFDEIIAWAEALKPLRAAGAAV
selected fasta
>flavin reductase [JadY]
ATGCTGTCCGACGAAAGCGCGAAGACAGGCTGTCCGCGAAATCGCCGCCAGGTCCACCGC
GCCATCCACCGCGCAAAACCCGGATCCGAAGCAGAGGGAGCACCACATGACCCGGATCGC
CGTCATCATCGGCAGCACCGTCCCGGCCGCCGCTCGACCGCCGTCGCCCAGTGGGTCGAG
GAGGCCGCGGGCCGCCACGAGGCGATCAAGGCCGGCGGCGCCACCGTCGAGCTCGTCGAC
ATCGCCGAGTTCGGCCTGCCGCTGCTCGACGAGTCCGTCCCGGCCGCCTTCGGCCAGTAC
GCCAACGAGCACACCAAGCGCTGGTCCGAGAAGATCGCGTCCTTCGACGCCTTCGTCTTC
GTCACCCCGGAGTACAACCGCTCCGTCCCGGCCGCGCTCAAGAACGCCATCGACTACCTG
TACGCCGAGTGGCAGAACAAGTCCGCCGGCTTCGTCAGCTACGGCGTCCAGGGCGGCACC
CGCGCCATCGACCACCTGCGGGTCACCCTCGCCGAGGTCAAGGTCGCCGACGTGCCGACC
GCCGTCTCGCTGCCGGTCTTCACCGACTTCACCTTCGAGAACCCCATGGACCCGACCGAG
ACCGGTCGCGTCACCCCCGGCCCGCACCAGGAGCCCACGCTCTTCAAGATGTTCGACGAG
ATCATCGCCTGGGCCGAGGCCCTCAAGCCGCTCCGTGCCGCGGGGGCGGCCGTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005025 NADPH-dependent FMN reductase (Domain)
[46-183] 2.29999999999998e-35 PF03358
PF03358   FMN_red
SignalP No significant hit
TMHMM No significant hit
Jado_00280 Jado_00280   Jado_00300 Jado_00300
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