Kirro_00200 : CDS information

Location

Organism	Streptomyces collinus
Strain	Tü365
Entry name	Kirromycin
Contig	AM746336
Start / Stop / Direction	22,893 / 23,399 / + [in whole cluster] 22,893 / 23,399 / + [in contig]
Location	22893..23399 [in whole cluster] 22893..23399 [in contig]
Type	CDS
Length	507 bp (168 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	hypothetical protein
Gene
Gene (GenBank)
EC number
Keyword
Note	This ORF is not involved in kirromycin biosynthesis.
Note (GenBank)
Reference	ACC B0B500 PmId [18291322] Molecular analysis of the kirromycin biosynthetic gene cluster revealed beta-alanine as precursor of the pyridone moiety. (Chem Biol. , 2008) comment kirromycin生合成gene clusterの報告。論文中ではorf(-1)としている。 orf(-1)欠損株作製。 kirromycin生産に影響しないことから、kirromycin生産に関与しないと考察している。また、orf(-1)の上流のorfも二次代謝産物生合成遺伝子とは相同性を示さないためこのORFが境界と示唆している。

Sequence

>hypothetical protein [hypothetical protein]
MTRIDDTPPAWDERSQLTTFLDYARDTARAKCEGVSAENARKALLPGSPLMTMSGVINHL
RWVEYHWFQVVFLGEEDRGPWTDEDPDREMRIAVEFPLAQLLEEYAEQSARYRELVAGND
LDKRAEGTIRNGLHVDLRWILLHLTEETARHNGHLDILRELLDGTTGI

>hypothetical protein [hypothetical protein]
ATGACGAGAATCGACGACACACCGCCCGCGTGGGACGAGCGCTCCCAGCTCACCACGTTT
CTCGACTACGCACGGGACACCGCCCGCGCCAAGTGCGAGGGTGTCTCCGCGGAGAACGCC
CGCAAGGCGCTCCTGCCCGGCTCACCGCTGATGACCATGAGCGGAGTGATCAACCATCTC
CGCTGGGTCGAGTACCACTGGTTCCAGGTGGTCTTCCTCGGGGAGGAGGACCGGGGCCCC
TGGACGGATGAGGACCCCGACCGCGAGATGCGTATCGCCGTCGAATTCCCGCTGGCGCAG
TTGCTCGAGGAGTACGCCGAACAGAGCGCCCGCTACCGCGAACTGGTCGCCGGGAACGAC
CTGGACAAGCGGGCTGAGGGAACCATCCGCAACGGCCTCCACGTCGACCTGCGCTGGATC
CTGCTGCACCTCACCGAGGAGACAGCCCGCCACAACGGTCACCTCGACATCCTGCGCGAG
TTGCTCGACGGCACGACCGGTATCTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR007061 Protein of unknown function DUF664 (Family) PF04978 DUF664
SignalP	No significant hit
TMHMM	No significant hit

Kirro_00190 Kirro_00210