Kirro_00400 Kirro_00400   Kirro_00420 Kirro_00420

Kirro_00410 : CDS information

close this sectionLocation

Organism
StrainTü365
Entry nameKirromycin
Contig
Start / Stop / Direction98,503 / 99,720 / + [in whole cluster]
98,503 / 99,720 / + [in contig]
Location98503..99720 [in whole cluster]
98503..99720 [in contig]
TypeCDS
Length1,218 bp (405 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative cytochrome P450
Product (GenBank)putative cytochrome P450 hydroxylase
Gene
Gene (GenBank)kirOII
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[18291322] Molecular analysis of the kirromycin biosynthetic gene cluster revealed beta-alanine as precursor of the pyridone moiety. (Chem Biol. , 2008)
comment
kirromycin生合成gene clusterの報告。

本ORFは機能解析実験されていない。
相同性より名付けられている。
KirOIとKirOIIが、C-16とC-30のhydroxylationに直接関与するかどうか、あるいは、tetrahydrofuran環の形成に関与するかを検証するためのmutation実験が今後行われると記述有り。
Related Reference
ACC
Q00441
PmId
[2011746] An erythromycin derivative produced by targeted gene disruption in Saccharopolyspora erythraea. (Science. , 1991)
[1732208] Characterization of Saccharopolyspora erythraea cytochrome P-450 genes and enzymes, including 6-deoxyerythronolide B hydroxylase. (J Bacteriol. , 1992)
[7749919] Structure of cytochrome P450eryF involved in erythromycin biosynthesis. (Nat Struct Biol. , 1995)
comment
accharopolyspora erythraea_eryF(CYP107A1)
6-deoxyerythronolide B hydroxylase

[PMID: 2011746](1991)
eryF(CYP107)破壊で6-deoxyerythromycin A産生。
よってeryFは6-deoxyerythronolide B (DEB) hydroxylaseをコードし、DEB → erythronolide B (EB)への変換を担う。
通常はEBで働く酵素が代替基質DEBを加工できたのでC-6 OH基を欠いた生物学的に活性のあるerythromycin派生体が形成された。

---
[PMID: 1732208](1992)
catalytically active cytochrome P-450 fractionより、majorなものとminorな種類の P-450 を精製し、各々の遺伝子orf405(major) とeryF(minor)の遺伝子をクローニングしている。また、各々の遺伝子をE. coliで発現させて活性を調べ、EryFは6-deoxyerythronolide B hydroxylase activityを持つのに対して、Orf405は6-deoxyerythronolide B hydroxylase activityを持たないこと、EryF, Orf405共に弱いながらも7-ethoxycoumarinのO-dealkylation活性を持つことが示されている。

---
[PMID: 7749919](1995)
Saccharopolyspora erythraeaのCytochrome P450eryFは6-deoxyerythronolide Bの6S-hydroxylationを触媒する(6-deoxyerythronolide Bを抗生物質erythromycinに変換するための第一ステップ)。Cytochrome P450eryFの結晶構造解析。

close this sectionSequence

selected fasta
>putative cytochrome P450 [putative cytochrome P450 hydroxylase]
MTGTFDWTDVEFDLSDVNFVTDREAGERWTGAPRPVCPGRFSDGADVYVVTRYEAVRSLF
ADRRVSNHPPEGVHLDSMRRRGVPEELLKYFDSTIMTMVPEDHRRVRSLIDRAFSVRRVK
SLRPRIERLADQLLDQMDPEGETDLVAGYAHPISTTVICELLGVDDEYRDQWLKWSEAFT
TFVRPDPEILPPALHGMVDTVIRLIGARRAQPGDDLISDLVQISDETEKLDEVELVALVL
VLVQAGLDTVRHSISLSFFNLLVHPDQLELVKSHPENTVQAVRELMRYSGPIKMALPRFA
AEPIEIDGVTIPKDGQIQLVVGAANNDPERFTDPRVLDVTRADNPQLSFAAGDHFCPGAS
LATAETEIALNKLFARYPDVRLAADPDEVGPRFLKAVTRLPVQLV
selected fasta
>putative cytochrome P450 [putative cytochrome P450 hydroxylase]
GTGACCGGAACATTCGATTGGACGGACGTCGAGTTCGACCTGTCCGACGTCAACTTCGTG
ACCGACCGCGAGGCGGGGGAGCGCTGGACAGGCGCGCCCCGGCCGGTGTGCCCGGGCCGC
TTCTCCGACGGCGCCGACGTCTACGTCGTCACCCGTTACGAGGCGGTCCGCAGCCTCTTC
GCCGATCGCCGGGTCAGCAACCACCCGCCGGAGGGCGTCCACCTCGACAGCATGCGCCGT
CGCGGCGTGCCCGAGGAACTGCTCAAGTACTTCGACTCGACGATCATGACGATGGTGCCG
GAGGACCACCGGCGCGTGCGCTCCCTGATCGACCGGGCGTTCTCGGTGCGCCGGGTCAAG
TCCCTGCGCCCCCGGATCGAGCGGCTCGCGGACCAGTTGCTCGACCAAATGGACCCCGAA
GGCGAGACGGACCTCGTCGCCGGGTACGCCCACCCGATCTCCACCACGGTCATCTGCGAA
CTCCTCGGCGTCGACGACGAGTACCGCGACCAGTGGCTGAAGTGGTCCGAGGCGTTCACC
ACCTTCGTCCGCCCCGACCCGGAGATCCTGCCGCCCGCGCTCCACGGCATGGTCGACACG
GTCATCCGGCTGATAGGCGCGCGCCGCGCCCAGCCCGGCGACGACCTGATCTCCGACCTG
GTCCAGATCAGCGACGAGACCGAGAAGCTCGACGAGGTCGAACTCGTCGCCCTGGTCCTG
GTCCTGGTGCAGGCCGGCCTCGACACCGTCCGGCACTCGATCTCGCTCAGCTTCTTCAAC
CTCCTGGTCCACCCCGACCAGCTGGAGCTGGTCAAGTCGCACCCGGAGAACACCGTCCAG
GCGGTCCGCGAGCTGATGCGCTACTCCGGTCCGATCAAGATGGCCCTGCCGCGCTTCGCC
GCGGAGCCGATCGAGATCGACGGGGTGACCATCCCCAAGGACGGCCAGATCCAGCTCGTG
GTCGGCGCCGCCAACAACGACCCTGAGCGGTTCACCGACCCGCGCGTCCTCGACGTCACC
CGCGCCGACAACCCCCAGCTCAGCTTCGCCGCCGGCGACCACTTCTGCCCGGGGGCGTCG
CTGGCCACGGCGGAGACCGAGATCGCGCTCAACAAGCTGTTCGCGCGCTACCCCGACGTG
CGCCTGGCCGCCGACCCCGACGAGGTCGGCCCGCGCTTCCTGAAAGCCGTCACACGACTG
CCCGTCCAGCTCGTGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[32-404] 1.9e-91 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
[280-377] 5.40000000000001e-15 PF00067
PF00067   p450
[35-404] 2.19999900980707e-81 SSF48264
SSF48264   Cytochrome_P450
IPR002397 Cytochrome P450, B-class (Family)
[98-109] 1.99999636034521e-39 PR00359 [144-160] 1.99999636034521e-39 PR00359 [161-176] 1.99999636034521e-39 PR00359 [199-221] 1.99999636034521e-39 PR00359 [280-291] 1.99999636034521e-39 PR00359 [298-325] 1.99999636034521e-39 PR00359 [326-341] 1.99999636034521e-39 PR00359 [347-356] 1.99999636034521e-39 PR00359 [356-367] 1.99999636034521e-39 PR00359
PR00359   BP450
SignalP No significant hit
TMHMM No significant hit
Kirro_00400 Kirro_00400   Kirro_00420 Kirro_00420
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