Lando_00020 Lando_00020   Lando_00040 Lando_00040

Lando_00030 : CDS information

close this sectionLocation

Organism
StrainS136
Entry nameLandomycin
Contig
Start / Stop / Direction2,584 / 3,858 / + [in whole cluster]
2,584 / 3,858 / + [in contig]
Location2584..3858 [in whole cluster]
2584..3858 [in contig]
TypeCDS
Length1,275 bp (424 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase beta-ketoacyl synthase subunit
Product (GenBank)B-ketoacyl-ACP synthase homolog
Gene
Gene (GenBank)lanA
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
  • LanA
Reference
ACC
PmId
[9933932] Cloning and characterization of a gene cluster from Streptomyces cyanogenus S136 probably involved in landomycin biosynthesis. (FEMS Microbiol Lett. , 1999)
comment
landomycin生合成gene clusterの同定論文。

lanA: beta-ketoacyl-ACP synthase

配列解析のみ。
urdamycin clusterのUrdA, jadomycin clusterのOrf1(JadA)に似ている。
Related Reference
ACC
Q54173
NITE
Urd_00120
PmId
[7592377] Cloning and characterization of a polyketide synthase gene from Streptomyces fradiae Tu2717, which carries the genes for biosynthesis of the angucycline antibiotic urdamycin A and a gene probably involved in its oxygenation. (J Bacteriol. , 1995)
comment
Blast 4th, id80%, 0.0
Streptomyces fradiae_urdA
Putative ketoacyl synthase

urdEFABCDを含むcosmid purd8をS.lividans TK24へ導入すると、urdamycin抵抗性を獲得。
urdamycin産生株S.fradiae Tu2717染色体から、urdE-C末+urdFABを含むfragment g領域を削除するとurdamycin産生不可。

AA配列解析で、UrdAはPKS gene clustersのbeta-ketoacyl synthasesに高い類似性あり。
UrdAとUrdBはid36%で似ているが、UrdBにはないactive site cysteineをUrdAは持っている。

TcmK ketoacyl synthase or TcmL chain length factorのどちらかを障害してtetracenomycin C非産生にしたStreptomyces glaucescensのmutantで、urdFABを異種性発現したらどちらのmutantも相補され、tetracenomycin Cを産生できた。

close this sectionPKS/NRPS Module

KS3..376

close this sectionSequence

selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [B-ketoacyl-ACP synthase homolog]
MGRRVVVTGIGVLAPGGIGAENFWSLLSEGRTATRGITFFDPSSFRSQIAAEADFDAERS
GLSPQEIRRMDRAAQFGVVTGREALADSGLDQAGLDPYRTGVTIGSAVGATMGLDEEYRV
VSDGGRLDLVDHHQYTPQHLYNHFVPSSFSAEVAWAVGAEGPNTVVSTGCTSGIDSVGQA
VELIREGSVDVMIAGATDAPISPITMACFDAIKATTPRNDEPEHASRPFDGTRNGFVLGE
GSAVFVLEELTAARARGANIYAEIAGYASRCNAFHMTGLRPDGREMGEAIRVALDEARIN
PEAIDYINAHGSGTKQNDRHETAAFKLSLGEHAYRTPISSIKSMVGHSLGAIGSIEIAAS
LLAMENHVVPPTANLHTPDPECDLDYVPLVAREHTTDHGPDGRQRIPVGFQSAMVLARPE
RSAA
selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [B-ketoacyl-ACP synthase homolog]
ATGGGACGTCGTGTAGTCGTCACCGGAATCGGAGTCCTCGCACCGGGCGGCATCGGGGCC
GAGAACTTCTGGAGTCTGCTCAGCGAGGGCCGCACGGCGACGCGGGGGATCACCTTCTTC
GATCCCTCCTCGTTCCGTTCGCAGATCGCCGCCGAGGCGGACTTCGACGCCGAGCGGAGC
GGGCTGAGCCCTCAGGAGATACGGCGGATGGACCGTGCCGCCCAGTTCGGAGTGGTCACC
GGGCGCGAGGCGCTGGCGGACAGCGGACTGGACCAGGCAGGCCTGGACCCCTACCGCACC
GGAGTCACCATCGGAAGCGCGGTCGGCGCCACCATGGGCCTCGACGAGGAGTACCGGGTG
GTCAGCGACGGCGGCCGACTCGACCTCGTCGACCACCACCAGTACACCCCGCAGCACCTG
TACAACCACTTCGTGCCGAGTTCCTTCTCGGCCGAGGTGGCGTGGGCCGTGGGGGCCGAG
GGACCCAACACCGTGGTGTCCACCGGCTGCACCTCAGGCATCGACTCGGTCGGCCAGGCC
GTCGAGCTGATCCGCGAGGGCAGCGTCGACGTGATGATCGCCGGTGCGACGGACGCCCCC
ATCTCGCCGATCACCATGGCGTGCTTCGACGCGATCAAGGCGACCACGCCCCGCAACGAC
GAGCCCGAGCATGCCTCGCGGCCCTTCGACGGGACCCGCAACGGCTTCGTGCTCGGCGAG
GGTTCGGCGGTGTTCGTCCTGGAGGAGCTGACCGCCGCCCGCGCCCGTGGCGCCAACATC
TACGCGGAGATCGCCGGCTACGCCTCGCGGTGCAACGCCTTCCACATGACCGGGCTGCGC
CCCGACGGACGTGAGATGGGGGAGGCGATCCGGGTCGCGCTGGACGAGGCCCGGATCAAC
CCCGAGGCCATCGACTACATCAACGCGCACGGCTCGGGCACCAAGCAGAACGACCGGCAC
GAGACGGCCGCGTTCAAACTCAGCCTCGGCGAGCACGCCTACCGGACCCCGATCAGCTCC
ATCAAGTCGATGGTGGGTCACTCACTGGGCGCCATCGGCTCGATCGAGATCGCGGCGTCC
CTGCTCGCCATGGAGAACCACGTGGTGCCGCCCACCGCGAACCTGCACACGCCCGACCCG
GAGTGCGACCTCGACTACGTCCCGCTCGTCGCCCGTGAGCACACCACGGACCACGGTCCT
GACGGTCGGCAGCGGATTCCGGTGGGATTCCAGAGCGCGATGGTGCTGGCCCGCCCGGAG
AGGAGCGCGGCATGA
KS3..376
KS7..1128

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[3-252] 5.40000000000002e-63 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[261-376] 6e-38 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[3-266] 5.50000000000008e-65 G3DSA:3.40.47.10 [271-396] 3.80000000000003e-44 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[2-258] 3.39999972437719e-67 SSF53901 [220-420] 2.19999900980707e-56 SSF53901
SSF53901   Thiolase-like
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[161-177] PS00606
PS00606   B_KETOACYL_SYNTHASE
SignalP
[1-19] 0.959 Signal
Eukaryota   
[1-20] 0.501 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Lando_00020 Lando_00020   Lando_00040 Lando_00040
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