Lando_00120 : CDS information

Location

Organism	Streptomyces cyanogenus
Strain	S136
Entry name	Landomycin
Contig	AF080235
Start / Stop / Direction	12,086 / 13,153 / + [in whole cluster] 12,086 / 13,153 / + [in contig]
Location	12086..13153 [in whole cluster] 12086..13153 [in contig]
Type	CDS
Length	1,068 bp (355 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative glucose-1-phosphate thymidylyltransferase putative dTDP-glucose synthase
Product (GenBank)	NDP-hexose synthetase homolog
Gene
Gene (GenBank)	lanG
EC number	2.7.7.24
Keyword
Note
Note (GenBank)	LanG
Reference	ACC Q9ZGC8 PmId [9933932] Cloning and characterization of a gene cluster from Streptomyces cyanogenus S136 probably involved in landomycin biosynthesis. (FEMS Microbiol Lett. , 1999) [16039521] Iteratively acting glycosyltransferases involved in the hexasaccharide biosynthesis of landomycin A. (Chem Biol. , 2005) comment [PMID:9933932](1999) landomycin生合成gene clusterの同定論文。 lanG: NDP-hexose synthetase 配列解析のみ。 LanGとLanZ2は、dNDP-hexose synthetasesをコードするとされるGraD、StrDに似ている。 -- [PMID:16039521](2005) lanZ2の機能確認の際に、lanG, lanZ2をE.coliで発現、精製。 LanGのみがD-glucose-1-phosphateを活性化できた。(data not shown)
Related Reference	ACC Q2HR55 NITE Sch475_00270 PmId [18051285] Cloning and expression of glucose-1-phosphate thymidylyltransferase gene (schS6) from Streptomyces sp. SCC-2136. (J Microbiol Biotechnol. , 2007) comment Blast 2nd, id69%, 1e-140 Streptomyces sp. SCC 2136_schS6 Putative dNDP-glucose synthetase schS6をE.coliで発現、精製、酵素活性測定。 glucose-1-phosphate thymidylyltransferase活性を示す。 dTTP + alpha-D-glucose-1-phosphate → dTDP-D-glucose + pyrophosphate

Sequence

>putative glucose-1-phosphate thymidylyltransferase [NDP-hexose synthetase homolog]
MKALVLSGGSGTRLRPFSHSMPKQLIPIANKPVLEHVLRNVRDLGVTEIGVIVGDRAEQI
QAVIGDGSALGVEITYIPQEAPLGLAHCVELARPFLGDDDFVMYLGDNMLPEGVERIADE
FTAGRPAAQVVVHKVPDPRAFGVAELAADGRVERLVEKPQEPKSDLALIGVYFFTPAIHK
AVAAIEPSARGELEITDAIQWLVANGDRFKANEYKGYWKDTGRIEDVLECNRKLLDDLKP
ATEGEVDAASTLIGTVVVEAGPKSSAPAIEGPVVIGAGSVVKDSHIGPHTSIGRDVRLTG
TSIEYSIALDDATISHVRDLHSSVIGRSASVGPSDRSTRHHRLVVGDHTRVEVAA

>putative glucose-1-phosphate thymidylyltransferase [NDP-hexose synthetase homolog]
ATGAAGGCTCTGGTCCTGTCGGGCGGGTCGGGAACCCGGTTGCGCCCCTTCAGCCACTCG
ATGCCGAAACAGCTCATACCGATCGCCAACAAGCCGGTCCTGGAGCACGTCCTCCGGAAC
GTCAGGGACCTCGGCGTGACGGAGATCGGCGTCATCGTCGGCGACCGCGCCGAGCAGATC
CAGGCGGTCATCGGCGACGGCTCCGCGCTCGGCGTGGAGATCACCTACATCCCGCAGGAG
GCGCCGCTCGGCCTCGCCCACTGCGTGGAACTCGCCCGGCCCTTCCTCGGCGACGACGAC
TTCGTGATGTACCTCGGCGACAACATGCTGCCCGAAGGCGTCGAGCGGATCGCCGACGAG
TTCACGGCCGGCCGCCCGGCCGCCCAGGTCGTGGTCCACAAGGTGCCGGACCCGCGTGCC
TTCGGGGTCGCCGAGCTGGCCGCGGACGGCAGGGTCGAGCGCCTGGTGGAGAAGCCGCAG
GAGCCCAAGAGCGATCTCGCGCTCATCGGCGTCTACTTCTTCACCCCGGCGATCCACAAG
GCCGTTGCCGCCATCGAGCCGAGCGCTCGCGGCGAACTGGAGATCACCGACGCCATCCAG
TGGCTCGTCGCCAACGGCGACAGGTTCAAGGCCAACGAGTACAAGGGCTACTGGAAGGAC
ACCGGACGCATCGAGGACGTCCTGGAGTGCAACCGGAAGCTCCTGGACGACCTGAAGCCG
GCCACCGAGGGCGAGGTCGACGCGGCAAGCACGCTCATCGGCACGGTCGTGGTGGAAGCG
GGTCCAAAGTCATCGGCTCCCGCTATCGAGGGCCCCGTCGTGATCGGCGCCGGATCGGTG
GTGAAGGACAGCCACATCGGCCCGCACACCTCCATCGGCAGGGACGTCCGGCTGACCGGC
ACGAGCATCGAGTACTCGATCGCACTGGACGACGCCACCATCTCCCACGTCCGCGACCTG
CACAGCTCCGTCATCGGGCGCTCCGCGTCGGTCGGTCCCTCCGACCGGAGCACCCGCCAT
CACCGGCTCGTCGTCGGTGACCACACCCGAGTCGAGGTAGCGGCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005835 Nucleotidyl transferase (Domain) PF00483 NTP_transferase IPR005908 Glucose-1-phosphate thymidylyltransferase, long form (Family) TIGR01208 RmlA_long IPR011004 Trimeric LpxA-like (Domain) SSF51161 Trimer_LpxA_like
SignalP	No significant hit
TMHMM	No significant hit

Lando_00110 Lando_00130