Lando_00130 : CDS information

Location

Organism	Streptomyces cyanogenus
Strain	S136
Entry name	Landomycin
Contig	AF080235
Start / Stop / Direction	13,150 / 14,130 / + [in whole cluster] 13,150 / 14,130 / + [in contig]
Location	13150..14130 [in whole cluster] 13150..14130 [in contig]
Type	CDS
Length	981 bp (326 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative dTDP-glucose 4,6-dehydratase
Product (GenBank)	NDP-hexose 4,6-dehydratase homolog
Gene	lanH
Gene (GenBank)	lanH14
EC number	4.2.1.46
Keyword
Note
Note (GenBank)	LanH
Reference	ACC Q9ZGC7 PmId [9933932] Cloning and characterization of a gene cluster from Streptomyces cyanogenus S136 probably involved in landomycin biosynthesis. (FEMS Microbiol Lett. , 1999) comment landomycin生合成gene clusterの同定論文。 lanH: NDP-hexose 4,6-dehydratase 配列解析のみ。 LanHはdehydratasesに似ているので、C-6 dehydroxylationに関与するdTDP-glucose 4,6-dehydrataseをコードすると示唆される。
Related Reference	ACC Q56173 NITE Grana_00110 PmId [7476861] Identification of Streptomyces violaceoruber Tu22 genes involved in the biosynthesis of granaticin. (Mol Gen Genet. , 1995) [9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998) comment Blast 17th, id67%, 1e-121 Streptomyces violaceoruber_graE(gra-orf17) dTDP-glucose 4,6-dehydratase (EC 4.2.1.46) -- [PMID: 7476861](1995) abstract ORF2はstrEに似ている。 E.coliでORF2を発現させると、dTDP-glucose→dTDP-4-keto-6-deoxy-D-glucoseへの変換を触媒することができた(controlの5倍)。よって、ORF2はdTDP-glucose dehydrataseをコードする。 -- [PMID: 9831526](1998) gra cluster genesのまとめ。renameあり。 ORF2→ORF17 ORF3→ORF16

Sequence

>putative dTDP-glucose 4,6-dehydratase [NDP-hexose 4,6-dehydratase homolog]
MKKILITGGAGFIGSHYVRTLLNDGYEDWKGAHVTVLDKLTYAGNRDNLPEAHPRLTFVQ
GDICDFELLLELLPGHDAVVHFAAESHVDRSLESAEEFVHTNVTGTQRLLDAVLATRVKR
VVHVSTDEVYGSIDEGSWTEEWPLAPNSPYSASKAASDLLARSYWRTHGLDLSITRCSNN
YGPYQHPEKLIPLFVTNLLEGEQVPLYGDGGNIREWLHVDDHCRAIDLVLNKGRAGEIYN
IGGGNEQTNRAITERLIALTGQDWSKVRHVPDRKAHDLRYSLDESKIREELGYAPRITFE
QGLADTVAWYHDNPGWWKGTKHNKQG

>putative dTDP-glucose 4,6-dehydratase [NDP-hexose 4,6-dehydratase homolog]
ATGAAGAAGATCCTGATCACCGGTGGCGCGGGCTTCATCGGTTCGCACTACGTGCGCACC
CTCCTGAACGACGGCTACGAGGACTGGAAGGGCGCCCACGTCACCGTCCTCGACAAGCTC
ACCTACGCCGGCAACCGCGACAACCTGCCCGAGGCGCACCCGCGCCTGACCTTCGTCCAG
GGCGACATCTGCGACTTCGAGCTGCTGCTCGAACTGCTGCCGGGACACGACGCGGTCGTC
CACTTCGCCGCCGAGTCGCACGTGGACCGCTCTCTGGAGTCCGCGGAGGAGTTCGTCCAC
ACCAACGTCACCGGCACCCAGCGACTGCTCGACGCGGTGCTCGCCACCCGAGTCAAGCGC
GTCGTCCACGTCTCCACCGACGAGGTCTACGGCTCCATCGACGAGGGATCCTGGACCGAG
GAGTGGCCGCTGGCGCCCAACTCCCCGTACTCCGCCTCCAAGGCCGCGTCCGACCTGCTC
GCACGCTCGTACTGGCGCACCCACGGCCTCGACCTCTCCATCACCCGGTGCTCCAACAAC
TACGGCCCGTACCAGCACCCGGAGAAGCTCATCCCGCTGTTCGTCACCAACCTGCTGGAG
GGTGAGCAGGTGCCGCTCTACGGCGACGGCGGCAACATCCGCGAGTGGCTGCACGTCGAC
GACCACTGCCGTGCGATCGACCTCGTCCTCAACAAGGGCCGGGCCGGGGAGATCTACAAC
ATCGGCGGCGGCAACGAGCAGACGAACCGGGCCATCACCGAGCGGCTCATCGCTCTGACG
GGACAGGACTGGTCCAAGGTCCGTCACGTCCCCGACCGCAAGGCGCACGACCTGCGCTAC
TCCCTCGACGAGTCGAAGATCCGCGAAGAGCTCGGCTACGCCCCGCGCATCACCTTCGAG
CAGGGCCTCGCCGACACCGTCGCCTGGTACCACGACAACCCCGGCTGGTGGAAGGGGACC
AAGCACAACAAGCAGGGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR005888 dTDP-glucose 4,6-dehydratase (Family) TIGR01181 DTDP_gluc_dehyt IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	No significant hit
TMHMM	No significant hit

Lando_00120 Lando_00140