Meili_00040 : CDS information

Location

Organism	Streptomyces nanchangensis
Strain	NS3226
Entry name	Meilingmycin
Contig	FJ952082
Start / Stop / Direction	3,487 / 4,683 / + [in whole cluster] 3,487 / 4,683 / + [in contig]
Location	3487..4683 [in whole cluster] 3487..4683 [in contig]
Type	CDS
Length	1,197 bp (398 aa)

Annotation

Category	3.3 modification reduction
Product	putative cytochrome P450
Product (GenBank)	cytochrome P450
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	orf4
Reference	ACC D6C453 PmId [20348291] Cloning of separate meilingmycin biosynthesis gene clusters by use of acyltransferase-ketoreductase didomain PCR amplification. (Appl Environ Microbiol. , 2010) comment Meilingmycin Biosynthesis Gene Clustersを同定した文献。 Table1. Orf4(398 a.a.): cytochrome P450 Orf4自体の機能実験は無し。詳細な記載は無く、meilingmycin biosynthesis への関与は不明。
Related Reference	ACC P33271 PmId [1732208] Characterization of Saccharopolyspora erythraea cytochrome P-450 genes and enzymes, including 6-deoxyerythronolide B hydroxylase. (J Bacteriol. , 1992) comment <Saccharopolyspora erythraea, BLAST 40, id=40%> orf405に相当するentry. catalytically active cytochrome P-450 fractionより、majorなものとminorな種類の P-450 を精製し、各々の遺伝子orf405(major) とeryF(minor)の遺伝子をクローニングしている。また、各々の遺伝子をE. coliで発現させて活性を調べ、EryFは6-deoxyerythronolide B hydroxylase activityを持つのに対して、Orf405は6-deoxyerythronolide B hydroxylase activityを持たないこと、EryF, Orf405共に弱いながらも7-ethoxycoumarinのO-dealkylation活性を持つことが示されている。

Sequence

>putative cytochrome P450 [cytochrome P450]
MSIIFDPRDPSVRTDPYPIYRRLRETDPIHQSHFGYWILSRYADVDAVLRAPGASSEFYR
DRTWANRRGGPDSPLVQSVRKWMLMLDGPAHRRIRGAISKVFTRAAVERLRPRIAAETAR
LLDAVGDGKTDLIHSLALPLPVTVTCELLGLPGSDRDQCRRWTEQISRVIDPSITAEDAV
EMNAAEVEFREYVADHLKERRSAPRDDILSLLLHAEVDGGRLTEAEIIANVQFLFVAGHE
TTVNLIGNGLLALLRHPDQLRMLRDNPEIIADSIDEITRYDPPVQIVSRLLTEDVPLADV
TLPAGAKVMLLFGAASRDPERYPDPDRLDLTRTGVKTLAFSGGPHYCIGAALGKLETSMV
LTELLQRYSTIELADDDLVWRPNVSFRGLQELPLQLVC

>putative cytochrome P450 [cytochrome P450]
ATGAGCATAATTTTCGATCCGCGAGATCCGTCGGTCCGTACCGACCCCTATCCCATCTAT
CGGCGCCTGCGCGAAACAGACCCCATCCATCAATCACACTTCGGCTATTGGATTCTTTCG
CGCTATGCCGATGTGGATGCCGTACTGCGCGCGCCAGGAGCGTCGAGCGAGTTCTACCGG
GACCGGACGTGGGCCAACCGCCGCGGTGGTCCGGACAGCCCTCTCGTGCAGAGCGTACGA
AAATGGATGCTCATGCTCGACGGCCCGGCTCATCGACGGATCCGCGGTGCGATCAGCAAG
GTTTTCACCCGGGCCGCCGTGGAACGGCTGAGGCCACGCATAGCCGCCGAGACCGCCCGG
CTGCTCGACGCCGTGGGCGACGGCAAGACGGACCTCATCCACAGCCTCGCCCTGCCCCTT
CCGGTGACTGTGACCTGCGAACTTCTCGGTTTACCCGGAAGCGATCGAGACCAGTGCCGC
CGCTGGACGGAACAAATCAGCCGTGTGATCGACCCCTCGATAACCGCCGAGGACGCGGTC
GAGATGAACGCGGCGGAGGTGGAATTTCGGGAGTACGTCGCCGACCATCTCAAAGAGCGC
AGGTCGGCGCCGCGCGACGATATTCTCTCCCTCCTTCTGCACGCCGAGGTCGATGGCGGG
CGGCTGACCGAGGCGGAGATCATCGCCAATGTTCAGTTCTTGTTCGTCGCAGGCCATGAG
ACCACCGTCAATCTGATCGGAAACGGCCTCCTGGCGCTGCTCCGCCATCCCGACCAGTTG
CGCATGCTGCGCGACAACCCGGAAATCATCGCCGACTCCATCGATGAGATCACTCGATAC
GACCCTCCCGTGCAGATCGTCTCCAGGCTCCTGACCGAAGACGTCCCCTTGGCTGATGTC
ACCCTCCCCGCGGGCGCCAAGGTGATGCTGCTTTTCGGGGCGGCCAGTCGTGACCCGGAG
CGCTATCCCGATCCCGACCGCCTCGACCTGACCCGCACCGGGGTGAAAACGCTGGCCTTC
AGCGGTGGACCGCATTACTGCATCGGTGCCGCGCTGGGGAAGTTGGAGACCTCGATGGTG
CTGACGGAGTTGCTGCAGCGGTATTCCACGATCGAGCTCGCCGACGACGACCTGGTCTGG
CGTCCGAACGTCAGCTTCCGCGGTCTGCAGGAACTGCCGCTCCAACTGGTGTGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PF00067 p450 SSF48264 Cytochrome_P450 G3DSA:1.10.630.10 Cyt_P450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	No significant hit
TMHMM	No significant hit

Meili_00030 Meili_00050