Meili_00040 Meili_00040   Meili_00060 Meili_00060

Meili_00050 : CDS information

close this sectionLocation

Organism
StrainNS3226
Entry nameMeilingmycin
Contig
Start / Stop / Direction10,563 / 4,699 / - [in whole cluster]
10,563 / 4,699 / - [in contig]
Locationcomplement(4699..10563) [in whole cluster]
complement(4699..10563) [in contig]
TypeCDS
Length5,865 bp (1,954 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.2 function unknown
Productpolyketide synthase
Product (GenBank)modular polyketide synthase
Gene
Gene (GenBank)pks2
EC number
Keyword
Note
  • This ORF is not involved in meilingmycin biosynthesis.
Note (GenBank)
Reference
ACC
PmId
[20348291] Cloning of separate meilingmycin biosynthesis gene clusters by use of acyltransferase-ketoreductase didomain PCR amplification. (Appl Environ Microbiol. , 2010)
comment
Meilingmycin Biosynthesis Gene Clustersを同定した文献。

Table S1
Pks2(1,954 a.a.): PKS
module3(KS-ATp-DH-KR-ACP) + TE

pks2を含む領域(pks2-orf15)をdeletionしてもmeilingmycin biosynthesisに影響は無いので、meilingmycin biosynthesisとは無関係であると考えられている。

close this sectionPKS/NRPS Module

close this sectionSequence

selected fasta
>polyketide synthase [modular polyketide synthase]
MADDRLIDTVQRLTIALRDTRERLRVAEAGAREPIAIVGAGCRFPGGVSDPEGLWRLVGE
GRDAVTAFPTDRGWPDEFTGAGAFLTDPAWFDAGFFGIGPREALSMDPQQRLMLEISWEA
VERAGIDPLSLRGSRTGVFTGTNDQDYRGLLGKVSNHNEHADHLATGTAAGVLSGRVAYF
LGLEGPSATVDTACSSSLVALHLAVRSLRSGECDLALAGGVVVLSTPGLFTAFRRQGALA
ADGRCKAFAEAADGVGWGEGAGVVLVERLTDALAHGHPVLAVVRGTAINSDGASNGLTAP
NGLAQQRVIRDALTDAGLRGRDVDAVEAHGTGTRLGDPIEASALQAAYGGDRETPLAIGS
VKSNIGHTQAAAGIAGVLKTVVALQNRTLPQTLHVDTPSSQVDWDLDTVELLTESRRWPA
GPGRPRRAGVSAFGISGTNAHVILEEAPPAEATPATEAQPLPWVLSARTDSALRARAGQL
LSHLDSRDATGVAHALVTSRSTFERRAVVLDTDPRTGLTALSRGGTVPGLITGAADLTGK
VVFVFPGQGGQWAGMAGDLLETSPVFREHIHACAQALAPHVDWDLPDVLRQRPDAPSLER
VDVVQPALFAVMVALAALWRSCGIEPDAVVGHSQGEIAAAHVAGALSLQDAALVVARRAK
ALTRLSGSGGMASVELPADELWPRLDERLCIATVNGPRATVIAGDTDALDVLLADLADSG
IRVRKLPVDYASHSPEMELVRDRVLSELAPISPQATDIPFHSAVTGAVLAGTELDAGYWY
RNLRLPVRFDQAVAGLAAPEHSFFVEIGPHPVLGPAVLEAVGDKAVVVGSLRRGEDGRRA
LLSSLAELYVRGLCPDWTAWAGPDTPGFTELPTYPFERKRFWLTPGESRSADHPVLDPAI
ELAQSDELVLTGRLSTESTPWLADHRVLDEVILPGTAWLEFALHAGAVANCATVEELTLA
VPLVLPDEGAATVQLRVGAPDDTGRRRLDLHAKVDGAGWVRHGEGVLSPAPTEPEALEKW
PPEGAEAVPHTGLYPALIGHGLGYGPTFQGLRTVWRRGDEVFCEVACPVDTHGFVVHPAL
LDSALHALGFSTPAGGEAGESNGPVLPFTWTGVTAHTAGPSALRVRLRGSVDDGVRLTVT
DSEGAPVLSVDSLVLRPAAVRSGAPRDALFRNRWVEVRPSGGLAAETMVAVGFETGHFPV
LPDLAALEEAVRAGLPVPGAVLVDGSHGSVRESAHRALSLVQWWCARPEFAASRLVFLTS
GAVAARTGDDVPDLAGAAVIGLVRSAQTEEPGRFVLLDADEITPQTITAALAADEPEVAL
RGGRLLARRLVRVVPRAPGTFAGPEDTVLITGGTGTLGRLLARHLVTAHGVRRLVLVGRR
GRAPGLRDELGALGATVTVAACDVADRTQLAQLLGAHRPTVVVHAAGVLDDGVVTAMNED
RVDVVLRPKAEAALALHELTQDLPLTSFVLFSSAAATAGSAGQSNYAAANAVLDALAHHR
RARGLPAVSLAWGLWQERSDMTRHLDSTATPPSTADSLSTTEALALFDAAAALPDPVLVP
ARLDLRGRQVGPLLRELVNRPEIPEDNPRQLLDLLAEPERERALLALVLDKVALVLGHDG
TDELGPHAGFLEMGVDSLAAVHIRNHLSQALDVRLRATVTFDHPTPSALVRHLTEALARP
EGEPAPEEHGETVDELIARTRATAWAQTRTYFTSPAELPRIPRPTRLAGGPEGPALVCVT
SAVGTSDPVQYARLAKPFQGERDVWALRQPGFHRGDQLPRTPETLLETHAASLRAELGER
PFVLAGLSSGGLIAHMLARYLYDQGAPPAGVILLDSYAPGEDERLALLAPGLRGELQHRA
DDPDSAAVPSDDSWVTAMLHYETFDWPFTELPAPVLFVRAGEPLESWPADWDWQPVWPYD
HTAVITRGNHFTMLEEHAPHTAALMRDWMRATFD
selected fasta
>polyketide synthase [modular polyketide synthase]
ATGGCTGACGACAGGCTCATCGACACCGTGCAGCGCCTGACCATCGCGCTGCGGGACACC
CGGGAACGGCTGCGGGTGGCCGAGGCCGGCGCGCGTGAGCCGATCGCCATCGTGGGGGCG
GGCTGCCGGTTCCCCGGCGGAGTGAGCGACCCGGAAGGGCTGTGGCGGCTGGTCGGCGAG
GGCCGGGACGCCGTCACGGCCTTTCCTACCGACCGCGGCTGGCCGGACGAGTTCACCGGC
GCGGGGGCGTTTCTGACCGACCCGGCCTGGTTCGACGCGGGCTTCTTCGGCATCGGCCCC
CGCGAGGCCCTGTCGATGGATCCGCAACAGCGGCTGATGCTGGAAATCTCCTGGGAAGCG
GTGGAACGGGCCGGAATCGACCCGCTGTCCCTGCGCGGCTCCCGGACCGGGGTGTTCACG
GGCACCAACGACCAGGACTACCGCGGCCTGCTGGGCAAGGTCAGCAACCACAACGAACAC
GCCGACCACCTGGCCACCGGCACCGCGGCCGGCGTGCTGTCCGGGCGAGTCGCCTACTTC
CTCGGGCTGGAGGGCCCGTCGGCCACGGTCGACACCGCGTGCTCGTCCTCGCTGGTGGCG
TTGCATCTGGCCGTCCGGTCGCTGCGCTCGGGGGAATGCGATCTCGCACTGGCCGGCGGC
GTCGTCGTGCTGTCCACGCCCGGACTGTTCACCGCTTTCCGGCGCCAGGGCGCGCTCGCC
GCGGACGGCCGCTGCAAGGCGTTCGCCGAGGCGGCGGACGGCGTGGGCTGGGGCGAGGGC
GCCGGGGTCGTGCTCGTCGAGCGGCTGACCGACGCCCTCGCCCACGGACACCCGGTGCTG
GCCGTGGTACGGGGCACCGCGATCAACTCCGACGGCGCCTCCAACGGGCTGACCGCGCCG
AACGGGCTCGCACAGCAGCGGGTCATCCGCGACGCGCTGACCGACGCCGGGCTCCGCGGC
CGGGACGTGGACGCCGTCGAGGCGCATGGCACGGGGACCCGGCTCGGCGATCCGATCGAG
GCGTCGGCGTTGCAGGCGGCGTACGGTGGCGACCGGGAAACCCCGCTGGCGATCGGGTCG
GTGAAGTCGAACATCGGGCACACCCAAGCCGCCGCCGGTATCGCCGGCGTACTGAAGACC
GTAGTGGCGCTGCAAAACCGGACCCTGCCACAGACGCTGCATGTGGACACCCCGTCGTCG
CAGGTGGACTGGGACTTGGACACGGTGGAGCTGCTCACCGAAAGCCGCCGCTGGCCGGCC
GGCCCGGGCCGCCCCCGGCGCGCGGGAGTGTCCGCGTTCGGCATCAGCGGCACCAACGCC
CATGTGATCCTCGAAGAGGCGCCACCCGCCGAAGCGACACCGGCCACCGAAGCGCAGCCG
CTGCCATGGGTGCTCTCGGCACGCACCGACTCCGCGCTCCGTGCCAGGGCGGGACAGTTG
CTCTCGCACCTCGACAGCAGGGACGCGACAGGCGTCGCCCACGCGCTGGTCACCTCCCGC
AGCACATTCGAGCGCCGTGCCGTGGTGCTCGATACGGATCCCCGGACCGGCCTGACCGCG
TTGAGCCGCGGCGGCACCGTACCGGGCCTGATCACCGGCGCCGCCGATCTGACGGGCAAG
GTGGTGTTCGTCTTCCCCGGCCAGGGCGGTCAGTGGGCGGGAATGGCCGGCGACCTGCTG
GAGACATCGCCGGTGTTCCGGGAGCACATCCACGCTTGCGCCCAGGCGCTGGCGCCCCAT
GTCGACTGGGACCTGCCGGACGTGCTGCGGCAGCGGCCGGACGCGCCCTCGCTGGAGCGA
GTGGACGTGGTGCAACCGGCGCTGTTCGCGGTCATGGTCGCGCTCGCCGCCCTCTGGCGC
TCCTGCGGGATCGAGCCGGATGCCGTGGTGGGACACAGCCAGGGGGAGATCGCCGCCGCC
CATGTGGCCGGCGCACTGTCCCTCCAGGACGCCGCGCTGGTGGTGGCCCGGCGCGCCAAG
GCGCTGACCAGACTCTCCGGCAGCGGCGGCATGGCTTCGGTGGAGCTGCCGGCGGACGAA
CTGTGGCCCCGGCTGGACGAGAGGCTGTGCATCGCGACGGTCAACGGGCCCAGGGCGACG
GTGATCGCCGGAGACACCGACGCGCTCGACGTACTGCTGGCGGACTTGGCCGACTCCGGC
ATACGGGTACGGAAGCTGCCGGTGGACTACGCGTCCCATTCGCCCGAGATGGAACTCGTC
CGCGACCGCGTCCTCAGCGAGCTGGCGCCGATCTCGCCGCAGGCCACGGACATCCCCTTC
CACTCGGCGGTGACCGGCGCCGTACTCGCCGGGACGGAGCTCGATGCCGGGTACTGGTAC
CGGAACCTGCGCCTTCCGGTGCGGTTCGACCAGGCCGTCGCCGGGCTCGCGGCGCCGGAA
CACTCGTTCTTCGTGGAGATCGGCCCGCACCCCGTACTGGGCCCGGCGGTCCTGGAGGCC
GTCGGCGACAAGGCCGTGGTCGTCGGATCGCTGCGCCGCGGGGAAGACGGCCGCAGGGCG
CTGCTCTCCTCGCTCGCCGAACTGTACGTCCGCGGTCTGTGCCCTGACTGGACGGCATGG
GCGGGGCCCGACACACCCGGATTCACCGAACTGCCCACCTATCCGTTCGAACGCAAGCGG
TTCTGGCTGACGCCGGGTGAGTCCAGAAGCGCCGACCATCCAGTGCTGGATCCGGCGATC
GAGCTGGCACAGAGCGACGAACTGGTGCTGACCGGACGCCTGTCGACGGAGTCGACCCCG
TGGCTGGCCGACCACCGCGTACTGGACGAGGTGATCCTGCCCGGCACCGCCTGGCTCGAA
TTCGCGCTGCACGCGGGCGCCGTGGCGAACTGCGCCACGGTCGAGGAACTGACCCTTGCG
GTGCCGCTGGTCCTGCCGGACGAGGGGGCCGCAACGGTGCAGCTGCGCGTCGGCGCACCG
GATGACACCGGGCGCCGCAGACTGGACCTCCACGCGAAAGTGGACGGCGCGGGGTGGGTG
CGGCACGGCGAAGGCGTGCTGAGCCCGGCACCCACCGAGCCCGAGGCGCTGGAGAAGTGG
CCGCCCGAAGGTGCGGAAGCGGTCCCGCACACCGGGCTCTACCCCGCGCTGATCGGCCAC
GGGCTGGGCTACGGCCCCACGTTCCAGGGGCTGCGCACCGTATGGCGCCGCGGCGACGAG
GTGTTCTGCGAGGTGGCCTGCCCGGTGGACACCCACGGCTTCGTGGTCCATCCGGCGCTG
CTCGACTCCGCCCTGCACGCTCTCGGCTTCAGCACCCCGGCGGGTGGCGAGGCAGGGGAG
AGCAACGGACCGGTGCTGCCGTTCACCTGGACCGGTGTCACCGCGCATACGGCTGGGCCC
TCGGCGCTACGGGTGCGGCTGCGCGGATCCGTCGACGACGGGGTGCGGCTGACCGTGACC
GACAGTGAGGGCGCGCCGGTGCTGTCGGTGGACTCGCTGGTGCTGCGCCCGGCGGCCGTA
CGCAGCGGCGCTCCACGGGACGCGCTGTTCCGGAACAGATGGGTCGAGGTGCGCCCTTCG
GGCGGGCTCGCGGCGGAAACCATGGTGGCCGTGGGCTTCGAGACCGGCCACTTCCCGGTT
CTCCCCGATCTCGCGGCGCTGGAGGAGGCGGTCCGCGCGGGTCTGCCGGTCCCGGGCGCG
GTGCTCGTCGACGGCTCCCACGGCTCGGTCCGCGAATCCGCACACCGCGCACTGAGCCTG
GTCCAATGGTGGTGTGCGCGCCCGGAGTTCGCCGCGTCCCGGCTGGTGTTCCTCACCTCG
GGCGCGGTGGCGGCCAGGACCGGCGACGACGTCCCCGATTTGGCGGGCGCGGCCGTCATC
GGGCTGGTGCGGTCGGCGCAGACCGAGGAACCCGGCCGGTTCGTCCTGCTGGACGCCGAC
GAGATCACCCCGCAGACGATCACCGCCGCCCTGGCCGCCGATGAACCGGAGGTCGCGCTG
CGGGGCGGACGGCTGCTGGCGCGCCGCCTGGTACGGGTCGTCCCCCGGGCCCCCGGAACG
TTCGCCGGGCCCGAGGACACCGTCCTGATCACCGGCGGCACCGGCACGCTCGGCCGGCTG
CTGGCCCGCCACCTGGTCACCGCGCACGGGGTGCGGCGGCTGGTCCTGGTCGGCCGTCGG
GGTCGGGCACCCGGGCTGCGGGACGAACTCGGCGCACTGGGCGCCACCGTCACCGTGGCC
GCCTGTGACGTGGCGGACCGGACACAACTGGCCCAACTGCTCGGCGCACACCGCCCCACG
GTCGTCGTCCACGCGGCCGGGGTGCTCGACGACGGCGTGGTGACGGCCATGAACGAGGAC
CGCGTCGACGTCGTCCTGCGGCCGAAGGCCGAGGCCGCGCTCGCCCTGCACGAGCTGACC
CAGGACCTGCCACTGACGTCCTTCGTGCTGTTCTCGTCGGCCGCGGCCACCGCGGGCAGC
GCGGGCCAGTCCAACTACGCCGCCGCCAACGCCGTACTCGACGCGCTGGCCCACCACCGG
CGCGCGCGAGGGCTGCCCGCGGTCTCGCTCGCCTGGGGCCTGTGGCAGGAACGCAGCGAT
ATGACCCGCCATCTCGACTCGACCGCCACCCCACCGTCCACCGCCGACTCGCTGTCCACC
ACTGAGGCGCTGGCGCTCTTCGACGCCGCGGCCGCGCTCCCCGACCCGGTGCTCGTGCCC
GCCCGGCTCGATCTGCGCGGCCGTCAGGTCGGGCCCCTGCTGCGGGAGCTGGTGAACCGA
CCGGAGATCCCGGAGGACAATCCGCGTCAGCTGCTCGACCTTCTGGCCGAACCGGAGCGC
GAACGGGCGCTGCTCGCCCTGGTGCTGGACAAGGTCGCGCTCGTCCTTGGACACGACGGC
ACGGACGAGCTCGGACCGCACGCCGGATTTCTGGAGATGGGCGTGGACTCACTGGCCGCG
GTCCACATCCGCAACCACCTCAGCCAGGCCCTCGACGTACGGCTGCGGGCGACCGTCACC
TTCGACCATCCGACCCCGTCGGCGCTGGTCCGGCATCTGACGGAGGCGCTGGCCCGGCCG
GAGGGCGAACCGGCCCCCGAGGAGCACGGCGAGACCGTCGACGAGCTGATCGCCCGGACC
AGGGCCACCGCCTGGGCACAGACGCGCACCTACTTCACCTCGCCGGCCGAGCTGCCGCGG
ATCCCGCGACCGACCCGGCTGGCCGGCGGCCCGGAAGGTCCGGCGCTGGTGTGTGTCACC
TCGGCCGTCGGCACCTCGGACCCGGTGCAGTACGCGCGCCTGGCGAAGCCGTTCCAGGGC
GAGCGGGACGTCTGGGCACTGCGGCAACCGGGATTCCACCGCGGCGACCAGCTCCCCAGG
ACCCCGGAAACGCTGCTCGAGACGCACGCCGCCAGTCTGCGCGCGGAGCTGGGGGAGCGG
CCCTTCGTACTCGCCGGCCTGTCCTCCGGCGGCCTGATCGCCCATATGCTCGCCCGGTAC
CTCTACGACCAGGGCGCCCCGCCCGCCGGTGTCATCCTGCTGGACAGCTACGCCCCCGGC
GAGGACGAACGGCTCGCCCTGCTGGCCCCCGGTCTGCGGGGGGAGCTCCAGCACCGGGCC
GACGACCCGGACTCCGCCGCGGTGCCCAGCGACGACAGCTGGGTCACCGCCATGCTGCAC
TACGAGACATTCGACTGGCCGTTCACCGAGCTGCCGGCCCCGGTGCTGTTCGTACGCGCG
GGGGAACCCCTGGAGAGCTGGCCGGCGGACTGGGACTGGCAACCCGTCTGGCCGTACGAC
CACACCGCCGTCATCACCCGCGGCAACCACTTCACCATGCTGGAGGAACACGCGCCGCAT
ACGGCCGCCTTGATGCGCGACTGGATGCGGGCCACCTTCGACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001031 Thioesterase (Domain)
[1735-1948] 1.99999999999999e-32 PF00975
PF00975   Thioesterase
IPR001227 Acyl transferase domain (Domain)
[533-665] 2.30000000000004e-76 G3DSA:3.40.366.10 [730-843] 2.30000000000004e-76 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR006162 Phosphopantetheine attachment site (PTM)
[1632-1647] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1597-1711] 1.39999892049878e-19 SSF47336
SSF47336   ACP_like
[1602-1677] 9.20000000000002e-17 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1600-1674] PS50075
PS50075   ACP_DOMAIN
[1608-1673] 3.5e-12 PF00550
PF00550   PP-binding
IPR013968 Polyketide synthase, KR (Domain)
[1347-1517] 7.10000000000008e-55 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[33-272] 1.9e-84 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[280-396] 2.19999999999997e-45 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[542-852] 3.19999999999996e-103 PF00698
PF00698   Acyl_transf_1
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[540-845] 4.60000044330866e-68 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[667-729] 5.00000909915354e-17 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[35-284] 2.79999999999994e-85 G3DSA:3.40.47.10 [285-449] 2.50000000000001e-64 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[25-394] 7.19996395544006e-97 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1346-1535] 1.69999999999999e-32 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[185-201] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[544-835] 9.69997533354407e-121 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1605-1677] 1.10000150671642e-23 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
[893-1051] 8.6000633021393e-65 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[35-449] SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1346-1518] 3.89999861795218e-54 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit
Meili_00040 Meili_00040   Meili_00060 Meili_00060
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