Merid_00050 : CDS information

Location

Organism	Streptomyces violaceusniger
Strain	DSM 4137
Entry name	Meridamycin
Contig	DQ885223
Start / Stop / Direction	86,051 / 87,277 / + [in whole cluster] 86,051 / 87,277 / + [in contig]
Location	86051..87277 [in whole cluster] 86051..87277 [in contig]
Type	CDS
Length	1,227 bp (408 aa)

Annotation

Category	3.3 modification reduction
Product	putative cytochrome P450
Product (GenBank)	MerD
Gene
Gene (GenBank)	merD
EC number
Keyword	C-9
Note	The N-terminal position was modified from original INSDC entry.
Note (GenBank)	putative P450 hydroxylase
Reference	ACC A0FCL4 PmId [17159202] Organization of the biosynthetic gene cluster in Streptomyces sp. DSM 4137 for the novel neuroprotectant polyketide meridamycin. (Microbiology. , 2006) comment meridamycin biosynthetic gene clusterを同定した文献。 merD(408a.a): cytochromoe P450 monooxygenase merD自体の機能実験は無いが、相同性よりcytochrome P450 hydroxylaseであることを予想しており、meridamycinの構造から推測してhydroxylationが必要なのはC-9の位置以外無いことから、C-9のoxidationに関与している可能性を予想している。
Related Reference	ACC P33271 PmId [1732208] Characterization of Saccharopolyspora erythraea cytochrome P-450 genes and enzymes, including 6-deoxyerythronolide B hydroxylase. (J Bacteriol. , 1992) comment <Cytochrome P450 107B1, Saccharopolyspora erythraea, BLAST 63, id=43%> orf405に相当するentry. catalytically active cytochrome P-450 fractionより、majorなものとminorな種類の P-450 を精製し、各々の遺伝子orf405(major) とeryF(minor)の遺伝子をクローニングしている。また、各々の遺伝子をE. coliで発現させて活性を調べ、EryFは6-deoxyerythronolide B hydroxylase activityを持つのに対して、Orf405は6-deoxyerythronolide B hydroxylase activityを持たないこと、EryF, Orf405共に弱いながらも7-ethoxycoumarinのO-dealkylation活性を持つことが示されている。

Sequence

>putative cytochrome P450 [MerD]
MEPRPIVLDPAGRDIPGEAARVAAGGEATLVELPDGVVAWAISRQRTLKQLLTDPRVSKD
PRQHWPAWINGEISPEWPLITWVAVENMFTSYGGDHRRLRTLVSKAFTARRTAALRPRIE
EICATLLDGLATAAPGEPVDLREAYAYPLPIQVISELFGLDDENLRERMRRVVDSIFHTS
ATPEEVTATYTEMYAVLGELVATKRERPGDDLTSGLIAVRDEESGSALSEKELTDTLALF
LSAGHETTVNLLDNAIHALLTRPEQLDHVRAGRATWDDVIEETLRHSAPVANLPLRYAVE
DIELDGGVVLRKGDAILAAYAAAGRDPEVHGEDADHFDVTRGLKNHLAFGHGVHLCVGAP
LGRLEAAIALPALFDRFPGLKLASGQDPLEPVESFISNGHRTLPARLS

>putative cytochrome P450 [MerD]
ATGGAACCCCGTCCCATCGTTCTCGACCCCGCCGGCCGCGACATCCCCGGAGAGGCAGCC
CGAGTCGCAGCAGGCGGCGAGGCGACGCTTGTTGAGCTTCCTGACGGCGTGGTGGCGTGG
GCAATAAGCCGCCAGCGGACACTCAAACAGCTGCTGACCGACCCCCGGGTCTCCAAGGAC
CCCCGGCAGCACTGGCCGGCGTGGATCAACGGTGAGATCTCGCCCGAGTGGCCGCTGATC
ACCTGGGTCGCCGTCGAGAACATGTTCACCTCCTACGGCGGTGACCACCGGCGGCTGCGC
ACCCTGGTCTCCAAGGCGTTCACCGCCCGGCGCACCGCCGCCCTGCGGCCGCGCATCGAG
GAGATCTGCGCCACCCTCCTGGACGGGCTGGCCACCGCCGCGCCGGGCGAGCCGGTCGAC
CTGCGCGAGGCGTACGCGTATCCGCTGCCGATCCAGGTGATCTCCGAGCTGTTCGGGCTC
GACGACGAGAATCTGCGCGAGCGGATGCGCCGGGTCGTGGACTCCATCTTCCACACCTCC
GCCACGCCGGAGGAGGTCACCGCCACCTACACCGAGATGTACGCCGTACTCGGCGAGCTG
GTGGCCACCAAGCGCGAGCGCCCCGGCGACGACCTGACCAGCGGGCTGATCGCGGTGCGG
GACGAGGAGAGCGGCTCCGCGCTCAGCGAGAAGGAGCTGACCGACACCCTCGCGCTGTTC
CTCTCGGCCGGTCACGAGACCACGGTCAACCTCCTGGACAACGCCATCCACGCGCTGCTC
ACCCGGCCCGAGCAGCTCGACCACGTCCGGGCCGGCCGGGCCACCTGGGACGATGTGATC
GAGGAGACGCTGCGGCACTCCGCCCCGGTCGCCAACCTGCCGCTGCGCTACGCGGTGGAG
GACATCGAACTGGACGGCGGCGTGGTGCTGCGCAAGGGTGACGCGATCCTGGCCGCCTAC
GCGGCAGCCGGACGCGACCCCGAGGTGCACGGCGAGGACGCGGACCACTTCGATGTGACC
CGCGGGCTCAAGAACCACCTGGCGTTCGGCCACGGCGTCCATCTGTGCGTCGGCGCCCCG
CTGGGCCGGCTGGAGGCCGCGATCGCGCTCCCGGCCCTCTTCGACCGCTTCCCCGGGCTC
AAGCTGGCGTCCGGGCAGGATCCGCTGGAGCCGGTGGAGTCCTTCATCTCCAACGGTCAC
CGCACCTTGCCCGCACGGCTGTCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PF00067 p450 SSF48264 Cytochrome P450 PR00385 P450 G3DSA:1.10.630.10 no description IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	No significant hit
TMHMM	No significant hit

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