Ncarz_00010 : CDS information

Location

Organism	Streptomyces carzinostaticus
Strain	ATCC 15944
Entry name	Neocarzinostatin
Contig	AY117439
Start / Stop / Direction	10,097 / 10,894 / + [in whole cluster] 10,097 / 10,894 / + [in contig]
Location	10097..10894 [in whole cluster] 10097..10894 [in contig]
Type	CDS
Length	798 bp (265 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose C-4 ketoreductase
Product (GenBank)	NDP-hexose-4-ketoreductase
Gene	ncsC4
Gene (GenBank)
EC number
Keyword	deoxy aminosugar
Note
Note (GenBank)
Reference	ACC Q84HD2 PmId [15797213] The neocarzinostatin biosynthetic gene cluster from Streptomyces carzinostaticus ATCC 15944 involving two iterative type I polyketide synthases. (Chem Biol. , 2005) comment Neocarzinostatin生合成遺伝子クラスターの報告。 ncsC4: dNDP-hexose 4-ketoreductase 相同性より機能付けしている。 dNDP-2-amino-4-keto-6-deoxyhexoseの還元によりC-4位のヒドロキシル基をエピメリ化すると推測している。
Related Reference	ACC O33708 NITE Adria_00200 PmId [9209071] Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine. (J Bacteriol. , 1997) [10631513] A two-plasmid system for the glycosylation of polyketide antibiotics: bioconversion of epsilon-rhodomycinone to rhodomycin D. (Chem Biol. , 1999) [9447597] Production of the antitumor drug epirubicin (4'-epidoxorubicin) and its precursor by a genetically engineered strain of Streptomyces peucetius. (Nat Biotechnol. , 1998) comment Streptomyces peucetius Thymidine diphospho-4-keto-2,3,6-trideoxyhexulose reductase_dnmV [PMID: 9209071] dnmV: 4-keto基の還元 dnmV不活化mutant産物解析と相補による回復から、daunosamine生合成に関与することは確認されている。dTDP-4-ketodeoxyhexulose ketoreductase機能の割り当ては配列解析に基づく。 -- [PMID: 10631513] 異種性ホストにおけるL-daunosamineの生合成と付着のための最少の情報は、dnmLMJVUTS genesによってコードされる。 DnmV とavrE and eryBIV genesの産物は、DNR and DXRに由来する4'-epimeric anthracyclinesの合成を通して、dTDP-4-ketohexulose reductasesであることが証明されている(ref26)。 ↓そのref26 [PMID: 9447597] S.peucetius dnmV mutantに、Streptomyces avermitilis avrE or Saccharopolyspora eryBIV genesを異種性に導入すると、epirubicin (4'-epidoxorubicin) and 4'-epidaunorubicinを形成。 Saccharopolyspora eryBIV = dTDP-4-keto-6-deoxy-L-hexose 4-reductase

Sequence

>putative NDP-hexose C-4 ketoreductase [NDP-hexose-4-ketoreductase]
MPVDLASDSVGRLSESLAAAAPDALVNCVGATGGDPVRLAEVNARGPAVLCAPLLRARPT
ARLVHLGSAAEYGPQTDRVPASESAATRPVTVYGATKLAGTVTVSDSGLDALVLRVGNPV
GAGAPASGLPGRVCALLRKTGTGPDVVLRLGDLSAYRDFVDVRDVARAVERAVTAPGPLP
PVLNVGGGEAVLGRDLVHRLADTAGFRGRIEEQGTGSARSEHISWQCSDISAAAEVLGWQ
PTYSLARSLEALWSAGAHLEGDRPA

>putative NDP-hexose C-4 ketoreductase [NDP-hexose-4-ketoreductase]
GTGCCCGTCGACCTGGCATCCGACTCCGTCGGCCGACTGTCCGAGAGTTTGGCCGCGGCA
GCGCCCGACGCCCTCGTCAACTGCGTCGGAGCGACTGGTGGCGACCCGGTGAGGCTCGCG
GAAGTCAACGCCCGCGGCCCGGCCGTGCTCTGTGCGCCGCTGCTCCGAGCCCGTCCCACC
GCCCGCCTGGTGCATCTGGGGTCAGCCGCCGAGTACGGCCCCCAAACGGACCGGGTCCCC
GCGTCGGAGTCGGCGGCCACCCGTCCGGTGACCGTCTACGGTGCCACGAAACTGGCCGGC
ACGGTGACGGTGTCCGACTCGGGGCTGGACGCACTGGTCCTGCGAGTCGGCAACCCGGTG
GGGGCCGGCGCACCGGCCTCGGGACTGCCCGGCCGAGTCTGCGCTCTTCTGCGGAAGACG
GGCACCGGCCCCGACGTGGTGCTGCGCCTGGGCGACTTGTCGGCGTACCGGGACTTCGTC
GACGTGCGCGACGTGGCCCGGGCAGTGGAGCGGGCGGTGACCGCCCCCGGACCACTGCCG
CCCGTGCTCAACGTAGGCGGCGGGGAGGCCGTCTTGGGGCGCGATCTGGTGCATCGGCTG
GCGGACACTGCCGGCTTCCGCGGGCGCATCGAGGAACAGGGCACCGGTTCCGCCCGGTCG
GAGCACATCTCCTGGCAGTGCTCGGACATCTCCGCCGCCGCCGAGGTCCTCGGCTGGCAG
CCCACGTACTCGCTCGCCCGTTCGCTGGAGGCGCTGTGGTCGGCCGGAGCGCACCTGGAA
GGCGACCGTCCGGCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	No significant hit
TMHMM	No significant hit

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