Ncarz_00070 : CDS information

Location

Organism	Streptomyces carzinostaticus
Strain	ATCC 15944
Entry name	Neocarzinostatin
Contig	AY117439
Start / Stop / Direction	22,015 / 16,754 / - [in whole cluster] 22,015 / 16,754 / - [in contig]
Location	complement(16754..22015) [in whole cluster] complement(16754..22015) [in contig]
Type	CDS
Length	5,262 bp (1,753 aa)

Annotation

Category	1.1 PKS
Product	polyketide synthase
Product (GenBank)	iterative type I polyketide synthase
Gene	ncsB
Gene (GenBank)
EC number
Keyword	iterative naphthoic acid
Note
Note (GenBank)
Reference	ACC Q84HC6 PmId [15147895] Neocarzinostatin naphthoate synthase: an unique iterative type I PKS from neocarzinostatin producer Streptomyces carzinostaticus. (FEBS Lett. , 2004) [15797213] The neocarzinostatin biosynthetic gene cluster from Streptomyces carzinostaticus ATCC 15944 involving two iterative type I polyketide synthases. (Chem Biol. , 2005) [25238086] Insights into the programmed ketoreduction of partially reducing polyketide synthases: stereo- and substrate-specificity of the ketoreductase domain. (Org Biomol Chem. , 2014) comment [PMID:15147895] ncsBの単離と機能解析報告。 ncsBは真菌の6-methyl salicylic acid synthaseや、orsellinic acid synthases (AviM and CalO5)と相同性を示すORF。 NcsBの配列解析で、dicarboxylic acidのCoAエステルの結合選択性を示すconserved motif 674GHSVG678の活性残基S676を保存していた。その100残基下流で保存されたモチーフ776AFHS779の中にinvariantな残基Ala, Hisが保存されており、Hisに隣接するPheからmalonyl-CoA ATだと特定している。 neocarzinostatin naphthoate synthaseを持つプラスミドを含んだS. lividans TK24 と S. coelicolor YU105の培養で、neocarzinostatinのnaphthoic acid部分の重要な中間体である2-hydroxy-5-methyl-1-naphthoic acid を同定した。さらに、新規産物2-hydroxy-5-hydroxymethyl-1-naphthoic acid も単離している。 --- [PMID:15797213] Neocarzinostatin生合成遺伝子クラスターの報告。 ncsB: Type I PKS : KS, AT, KR, DH, ACP neocarzinostatin naphthoate synthase (NNS) --- [PMID: 25238086] 反復性PKSにおいて一部を選択的に還元するKRについての調査。
Related Reference	ACC P87162 PmId [20304931] ( , ) comment BLAST id40% Aspergillus terreus_atX 6-methylsalicylic acid synthase 6-methylsalicylic acid synthase_ATXのdehydratase(DH) domainの機能的検証。 DH domainはACPに繋がれたbeta-hydroxytriketide中間体のdehydrationに関連しないが、ACPから6- methylsalicylic acidを放出するためにthioester hydrolysisを触媒することが明らかになった。よってDH domainからTH domain(Gly904 - Thr1048の145aa)へ改名。構造解析報告のあるEryDH4とのalignmentから、H972XXXGXXXXP motif and Asp1129 が触媒に重要な残基であると提唱されている。著者らの既報で、ATX H972A mutantがbeta-hydroxy triketideのような産物をもたらさないことが確認されている。

PKS/NRPS Module

B	1		acetyl-CoA malonyl-CoA

KS	1..367

AT	526..841

TH	849..996

KR	1378..1571

ACP	1664..1733

Sequence

>polyketide synthase [iterative type I polyketide synthase]
MSCRYAPDLDSPDKFWDFLVNGRSTVGDMPDKRWEPYASGSPQATAAMRDTVRRGAFLDD
IEGFDAEFFGISPREADFLDPQQRFMLELAWEALADAGVPPLTLRNTDTGVYAAANSNDY
GRRLLEDIPRTGAYAVNGTTLYGIANRVSYFLDLHGPSMAVDTACAGALTALHLARQSLL
TGETPLAIVGGLNIMSTPSLNVALNDAGAMSPDGRSKAFDEDADGYGRGEGAGVLVLKRL
SDARRDNDPVHAVIRGSGVFQDGRSDGMMAPDGDAQEHMLRQAYHRAGVDPATVDYVEAH
GTGTPTGDREEITALAKVFGAGRSPHAPCLIGSVKPNVGHVEGGSGITGVIKTVLALRNE
LIPPTLHDRPRTDVDWDAWGVRLVGQVQEWPSCGRPRRAGVSSYGVGGTISHVILEESPV
PAATSSADASTGVRTPALFPLSAASEAGLRALAGEAAGWVASRPDTPLPSVGHTLTQRRS
HLAQRAAVVADSAEQLVDRLREVAEGRNGPGIVSARTSAGRADDAVWVFSGHGAQWSGMG
RRLLASEPVFAATLDALDEVFREELGWTPREAVTEGGPWTAAHVQALTFAVQIGLADVWR
SKGLRPGAVIGHSVGEIAAAVVAGSLDRDEAARFACRRAAALQRLDGRGAMVMVGLPFEE
AALRLGDRRDVEAAISAGPHSTVLSGDRSAVLRVAEEWQASEVWTRTVDSDIAFHSVHVD
EVTGDIESAARLLTPRPPTVPLYTTALSDPRSRAPRDSGYWAANLRKPVRFTEAVRAAAE
DGHRLFLEVSSHPVVAHSVSETLLDLGIEDAAVAGTLRRDTDEVESLLENLAELHCHGVA
VDWARHHTDGELVGLPAAVWQHRPYWIFPETTADAGLGRGHDPASHSLLGGRMTVSGSPT
RQVWQTRLDMDSRPYPQSHGLVGVEVTPAASIINTFAAAVEEDGPSALTDIVLRTPLAVE
PPRVVQVVREGRSLSLATRVAEDADADGSEWITHTTAAVTPGVRPAGGRLDTEAIRSRLP
EGSLTRADEMFERMGVEGYAFPWDLEELRHDDHEQLAVLQIEPSPAQRATSWAHVIDGAL
TISAMVVSPGDATVLWMSRSIDQVTWSGEPPARLTVHSTRSLRSPHDTVDVRVADERGDV
VCEVVGLRFAAVEHIGAAVLPSELVHEIVWRPWDPEDHEGAEDAPVEQVILVGDPEATVP
LAEQLESAGMTCVQVGDSPETGLRPDLFARPGAVVVAPALAGSDTAPEEEAERVSWLLVR
TVQRVAEIRADVTGDAPAQRVWCVTSDVRRARDERSVAHGPLWGLARIVAGDHPELWGGA
VDIGPSADGIGARLVALLDGAAGTEDVISLTGEGAEVARLSRIDRSADGTPLQCSPSGTV
LITGGLGALGLEVARWLVDRGARRLVLVSRRALPNRTEWPAVTDAETRRRIDGVLALEAL
GVTVRVLALDITDVDQVSAALAPEALGLPPVRGVVHAAGVVNNALVDKVDLEGLREVLAP
KVRGAMVLHRLFPPGDLDFFVLFSSCGQFARLSGQASYAAANSFLDTLASHRNAGEHTET
VSLGWTAWRGLGMSSNIDTTMFEANSRGLEAVSATEAFGAWSFGDRFQSDYQAILRVVPT
PVHTPRLPVFRDLPVSGETDGPTGDQLFTTTLEGLPEQEARERITADVREQVAGVLNFDP
SEVEVKRPLVELGVDSVMTVALRVRLQRRYGLELPPTILWAKPTVAALSEHVCDSLRWDG
EEHGDLAAPTAAA

>polyketide synthase [iterative type I polyketide synthase]
ATGAGTTGCAGGTATGCCCCGGATCTCGACTCGCCGGACAAGTTCTGGGACTTCCTTGTC
AACGGCAGAAGCACCGTCGGTGACATGCCTGACAAACGGTGGGAACCCTACGCATCGGGC
AGCCCGCAGGCGACCGCGGCGATGAGGGACACCGTTCGCCGCGGCGCGTTCCTCGATGAC
ATCGAAGGCTTCGACGCCGAGTTCTTCGGAATCTCGCCCAGGGAAGCCGACTTCCTGGAT
CCCCAGCAGCGCTTCATGCTCGAGCTCGCCTGGGAAGCCCTGGCCGACGCCGGTGTTCCC
CCGCTGACTCTGCGCAACACCGACACCGGCGTGTACGCCGCCGCGAATTCCAACGACTAC
GGCAGGCGGCTCCTGGAGGACATCCCGCGTACCGGTGCCTATGCGGTGAACGGCACGACG
CTGTACGGCATCGCCAACCGCGTCTCCTACTTCCTGGACCTCCACGGACCGAGCATGGCG
GTCGACACGGCCTGCGCCGGAGCGCTGACGGCTCTTCACCTCGCCCGTCAGAGCCTGCTG
ACCGGGGAAACGCCCCTGGCGATCGTCGGCGGTCTCAACATCATGTCCACTCCCTCCCTC
AACGTGGCCCTCAACGACGCCGGAGCCATGTCCCCCGACGGCCGCAGCAAGGCCTTCGAC
GAGGACGCCGACGGGTACGGCCGCGGTGAGGGGGCAGGGGTTCTCGTACTCAAGAGGCTG
TCCGACGCACGGCGCGACAACGATCCCGTCCACGCGGTCATTCGTGGCAGCGGTGTCTTC
CAGGACGGCCGTTCCGACGGCATGATGGCGCCGGACGGGGACGCCCAGGAGCACATGCTC
CGGCAGGCGTACCACCGGGCGGGGGTGGACCCGGCCACAGTGGACTACGTCGAGGCGCAC
GGCACGGGAACACCCACTGGTGACCGTGAGGAGATCACCGCCCTCGCCAAGGTGTTCGGA
GCAGGCCGGTCACCCCATGCCCCCTGCCTGATCGGATCGGTGAAGCCCAACGTCGGTCAC
GTCGAGGGCGGCTCCGGCATCACCGGCGTGATCAAGACGGTCCTCGCGCTGAGGAACGAG
CTGATCCCCCCCACCCTCCACGACCGGCCCCGTACCGACGTCGACTGGGACGCCTGGGGG
GTGCGCCTGGTCGGGCAGGTACAGGAGTGGCCCTCCTGCGGACGGCCCCGGCGCGCGGGA
GTCTCCAGCTACGGCGTCGGGGGCACGATCTCCCACGTGATCCTGGAGGAGAGCCCGGTG
CCGGCGGCGACCTCGTCCGCCGACGCCTCCACAGGCGTACGGACGCCCGCACTCTTCCCG
CTCTCGGCCGCCTCGGAGGCGGGACTGCGCGCGCTGGCGGGCGAGGCCGCAGGCTGGGTG
GCCTCTCGTCCTGACACCCCGCTGCCGTCGGTCGGACACACCCTGACTCAACGGCGCTCG
CACCTTGCTCAGCGTGCCGCGGTCGTCGCGGACTCCGCCGAGCAACTCGTCGACCGGCTG
CGCGAGGTCGCTGAGGGACGCAACGGCCCCGGCATCGTGTCGGCACGCACCAGTGCGGGG
CGCGCTGACGACGCCGTCTGGGTCTTCTCCGGGCATGGTGCCCAGTGGTCCGGAATGGGC
CGCCGACTGCTGGCGAGCGAGCCGGTCTTCGCGGCGACCCTCGACGCGCTGGACGAGGTG
TTCCGCGAGGAACTCGGCTGGACGCCCCGGGAGGCGGTCACGGAGGGCGGACCGTGGACT
GCCGCTCATGTCCAGGCACTGACCTTCGCCGTCCAGATCGGGCTGGCTGACGTGTGGCGC
AGCAAGGGCCTGCGGCCGGGCGCGGTCATCGGGCATTCCGTCGGTGAGATCGCGGCTGCG
GTGGTGGCCGGGTCCCTCGACCGTGATGAGGCCGCCCGTTTCGCGTGTCGCCGTGCGGCG
GCGCTCCAGAGGCTCGACGGGCGGGGCGCCATGGTCATGGTCGGGCTTCCGTTCGAGGAG
GCGGCCCTGCGCCTTGGAGACAGGCGGGACGTGGAGGCCGCCATCTCGGCGGGACCGCAT
TCCACGGTCCTGTCCGGTGACCGTTCGGCGGTTCTGCGCGTCGCCGAGGAGTGGCAGGCC
TCGGAGGTGTGGACGCGGACCGTCGACTCCGACATCGCTTTTCACAGCGTCCACGTCGAC
GAGGTGACTGGCGACATCGAGTCGGCCGCACGCCTGCTCACGCCCCGGCCGCCGACCGTC
CCCCTCTACACCACCGCCCTGTCCGACCCGCGGTCGCGGGCTCCGCGCGACAGCGGCTAC
TGGGCGGCGAACCTGCGCAAGCCGGTCCGCTTCACCGAGGCCGTACGCGCGGCCGCCGAG
GACGGCCACCGACTGTTCCTCGAGGTGTCCAGCCACCCGGTGGTGGCCCATTCGGTCAGC
GAGACGCTTTTGGACCTCGGCATCGAGGACGCCGCGGTGGCCGGGACACTGCGGCGCGAC
ACCGACGAGGTGGAGTCGCTGCTGGAAAACCTCGCCGAACTGCACTGCCACGGCGTGGCC
GTCGACTGGGCGCGCCATCACACCGACGGCGAGCTCGTGGGACTGCCGGCGGCCGTGTGG
CAGCACCGGCCCTACTGGATCTTCCCCGAGACCACCGCCGACGCGGGTCTCGGCCGTGGG
CACGATCCCGCGAGCCACAGCCTGCTCGGCGGCCGGATGACCGTCAGCGGCTCACCGACC
AGGCAGGTGTGGCAAACCCGGCTCGACATGGACAGCCGGCCCTACCCGCAGAGCCACGGG
CTCGTCGGTGTCGAGGTCACCCCCGCGGCGTCCATCATCAACACCTTCGCCGCAGCTGTG
GAGGAGGACGGTCCGTCCGCTTTGACGGACATCGTGCTGCGTACGCCCCTGGCGGTGGAA
CCGCCTCGGGTGGTCCAGGTGGTGCGCGAAGGAAGGTCGCTGTCGCTCGCCACCCGCGTG
GCCGAGGACGCCGACGCCGACGGGAGCGAGTGGATCACGCACACCACGGCCGCCGTCACG
CCGGGGGTCCGCCCGGCCGGCGGGCGGCTGGACACGGAAGCCATCCGCTCTCGGCTCCCG
GAGGGATCGCTCACCAGGGCCGACGAGATGTTCGAGCGCATGGGGGTCGAGGGCTACGCC
TTCCCGTGGGACCTGGAGGAGCTGCGCCACGACGACCATGAGCAGCTCGCCGTACTGCAG
ATCGAGCCCTCGCCGGCCCAGAGGGCGACCAGCTGGGCCCACGTCATCGACGGCGCGCTG
ACCATCAGCGCCATGGTGGTCTCCCCAGGGGACGCCACCGTGCTCTGGATGTCCCGGTCC
ATCGATCAGGTGACGTGGTCGGGTGAGCCGCCCGCCAGGCTCACCGTGCACAGCACCCGT
TCGCTCCGGTCACCGCACGACACGGTCGACGTGCGGGTCGCCGACGAACGGGGGGACGTC
GTCTGCGAGGTCGTCGGGCTCCGATTCGCGGCCGTCGAACACATCGGCGCCGCGGTATTG
CCCAGCGAACTGGTGCACGAGATCGTGTGGCGCCCCTGGGACCCGGAGGACCACGAGGGT
GCTGAGGATGCGCCCGTGGAGCAGGTGATCCTCGTCGGGGATCCCGAGGCCACCGTTCCT
CTCGCCGAACAACTCGAGTCGGCCGGCATGACCTGCGTACAGGTGGGCGACTCACCGGAG
ACGGGGCTGCGGCCCGACCTGTTCGCCCGGCCGGGCGCGGTGGTCGTTGCGCCCGCTCTG
GCCGGTTCGGACACGGCGCCGGAGGAGGAGGCGGAGCGCGTGTCGTGGCTGCTCGTACGG
ACCGTGCAACGTGTCGCCGAGATCCGGGCCGACGTCACCGGGGACGCACCGGCGCAGCGG
GTGTGGTGCGTCACCAGCGACGTCCGACGGGCCCGTGACGAACGGTCTGTGGCGCACGGG
CCGCTGTGGGGCCTGGCTCGCATCGTCGCGGGAGACCATCCCGAACTGTGGGGCGGGGCC
GTCGACATCGGGCCGTCGGCCGACGGCATCGGCGCGCGTCTGGTCGCACTGCTGGACGGT
GCCGCCGGTACGGAGGATGTCATCTCGCTGACGGGCGAGGGAGCCGAGGTCGCCCGGCTG
AGCCGCATCGACCGCAGTGCGGACGGTACGCCGCTGCAGTGTTCGCCCTCGGGCACCGTC
CTGATCACCGGCGGCCTGGGCGCTCTGGGACTGGAGGTCGCGAGGTGGCTGGTGGACCGG
GGCGCCCGTCGGCTCGTGCTCGTCAGCCGCCGTGCCCTGCCCAACCGTACCGAGTGGCCT
GCCGTCACCGACGCCGAGACCAGGCGCAGGATCGACGGCGTGCTGGCGCTCGAGGCACTC
GGCGTCACGGTCCGGGTGCTCGCGCTCGACATCACGGACGTCGACCAGGTGTCGGCGGCA
CTGGCGCCGGAAGCGCTCGGTCTGCCGCCGGTGCGGGGAGTCGTGCACGCTGCAGGTGTC
GTGAACAACGCCCTGGTGGACAAGGTCGACCTCGAGGGACTGCGAGAGGTGCTGGCCCCG
AAGGTCCGTGGCGCCATGGTGCTGCACCGCCTCTTCCCACCCGGTGATCTCGACTTCTTT
GTGTTGTTCTCCTCCTGCGGCCAGTTCGCCCGTCTGTCGGGCCAGGCCAGTTACGCCGCG
GCCAACTCGTTCCTGGACACGCTCGCGAGCCACCGCAACGCGGGAGAGCACACCGAGACG
GTCAGCCTGGGCTGGACGGCCTGGCGTGGGCTGGGCATGTCCTCGAACATCGACACCACG
ATGTTCGAGGCCAACTCGCGAGGCCTGGAAGCCGTCTCCGCGACCGAGGCGTTCGGCGCG
TGGTCCTTCGGCGACAGGTTCCAGTCCGACTACCAGGCCATACTCCGGGTGGTGCCGACC
CCCGTGCACACACCTCGCCTGCCGGTGTTCCGGGACCTGCCCGTCTCCGGCGAGACGGAC
GGCCCCACGGGCGACCAGCTCTTCACCACCACTCTGGAAGGGCTTCCGGAGCAGGAGGCA
CGCGAGAGGATCACCGCCGATGTGCGCGAACAGGTGGCCGGAGTGCTCAACTTCGACCCC
TCTGAAGTAGAGGTCAAGCGCCCCCTCGTCGAGCTCGGAGTCGACTCAGTGATGACGGTC
GCTCTCCGGGTGCGCCTGCAGCGTCGGTACGGCCTCGAGCTTCCGCCGACGATTTTGTGG
GCCAAGCCGACTGTGGCGGCCCTCTCCGAACATGTCTGCGACAGCCTGCGTTGGGACGGG
GAGGAGCACGGGGATCTGGCTGCGCCGACCGCGGCGGCGTAG

[1] KS	1..367

[1] AT	526..841

[1] acetyl-CoA malonyl-CoA	712..716

[1] TH	849..996

[1] KR	1378..1571

[1] ACP	1664..1733

[1] KS	1..1101

[1] AT	1576..2523

[1] acetyl-CoA malonyl-CoA	2134..2148

[1] TH	2545..2988

[1] KR	4132..4713

[1] ACP	4990..5199

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001227 Acyl transferase domain (Domain) G3DSA:3.40.366.10 Ac_transferase_reg IPR002198 Short-chain dehydrogenase/reductase SDR (Family) PF00106 adh_short IPR006162 Phosphopantetheine attachment site (PTM) PS00012 PHOSPHOPANTETHEINE IPR009081 Acyl carrier protein-like (Domain) G3DSA:1.10.1200.10 ACP_like PS50075 ACP_DOMAIN SSF47336 ACP_like PF00550 PP-binding IPR014030 Beta-ketoacyl synthase, N-terminal (Domain) PF00109 ketoacyl-synt IPR014031 Beta-ketoacyl synthase, C-terminal (Domain) PF02801 Ketoacyl-synt_C IPR014043 Acyl transferase (Domain) PF00698 Acyl_transf_1 IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain) SSF52151 Acyl_Trfase/lysoPlipase IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain) SSF55048 Malonyl_transacylase_ACP-bd IPR016038 Thiolase-like, subgroup (Domain) G3DSA:3.40.47.10 Thiolase-like_subgr IPR016039 Thiolase-like (Domain) SSF53901 Thiolase-like IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd IPR020801 Polyketide synthase, acyl transferase domain (Domain) SM00827 PKS_AT IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain) SM00823 PKS_PP IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain) SM00822 PKS_KR
SignalP	No significant hit
TMHMM	No significant hit

Ncarz_00060 Ncarz_00080