Ncarz_00260 : CDS information

Location

Organism	Streptomyces carzinostaticus
Strain	ATCC 15944
Entry name	Neocarzinostatin
Contig	AY117439
Start / Stop / Direction	47,373 / 45,715 / - [in whole cluster] 47,373 / 45,715 / - [in contig]
Location	complement(45715..47373) [in whole cluster] complement(45715..47373) [in contig]
Type	CDS
Length	1,659 bp (552 aa)

Annotation

Category	5.1 general function
Product	putative oxidoreductase
Product (GenBank)	oxidoreductase
Gene	ncsE9
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q84HA8 PmId [15797213] The neocarzinostatin biosynthetic gene cluster from Streptomyces carzinostaticus ATCC 15944 involving two iterative type I polyketide synthases. (Chem Biol. , 2005) comment Neocarzinostatin生合成遺伝子クラスターの報告。 ncsE9: FAD-dependent oxidoreductase ncsE-E11, ncsF1, ncsF2はenediyne core biosynthesisに関与する。このORFについて機能解析はされていない。
Related Reference	ACC P12676 PmId [2914858] Nucleotide sequence of the gene for cholesterol oxidase from a Streptomyces sp. (J Bacteriol. , 1989) [10194345] Crystal structure determination of cholesterol oxidase from Streptomyces and structural characterization of key active site mutants. (Biochemistry. , 1999) comment Streptomyces sp._choA Cholesterol oxidase EC 1.1.3.6/5.3.3.1 [25%id, 5e-18] [PMID:2914858] choA geneのシーケンス解析。 secreted cholesterol oxidaseであると推定された。 [PMID:10194345] choAの結晶構造解析活性中心が同定されている。
Family/Domain	FD IPR007867, IPR000172 comment [IPR007867] Glucose-methanol-choline oxidoreductase, C-terminal (Domain) [IPR000172] Glucose-methanol-choline oxidoreductase, N-terminal (Domain)

Sequence

>putative oxidoreductase [oxidoreductase]
MSGGAERTEVLVVGSGFGGAISAYHLAAGGAKVTVLERGPWLEHGQFEHDYKLGSSYTRI
FDFVVGDGMSVLGGNCVGGGSVVYFAAMPRAPRFVFDRKGSIGRRMWPKSISRNTLDPWY
DRVEESLSVARQGWQDVSYAGGLWAAACSHAGRTANPLPVAIDTVKCVNCNWMMAGCRFE
AKQSLLVNYLPAALAHGAEIRPLHEVQYLERTADGGYRVHYNVIDGEDYRTTAGSGVIEA
KIVVLAAGAGATPVILQRSEGQLGRMPHAVGRYFSGNGERLNTAVVDEDKAAELLGLDRG
SGPAYGANHIGRGPTVASWDRLDGSLPEYSRYSLEQLYFPPGLGTILAQVPHGTGPTWFG
ARKKEILKRWTSWLTVFSMIEDDNEGVFGPPPATGNAHRISQQLLGRGNLRYAPTQNTLN
AWAASDAEVKEILEKDGLAQVMPWTNDLVGAYTVHPLASCRMGDDPHTSALDDRNELRGH
PGIFVTDGSAVPGALTVNPALTISALAERAVPGIVEAAQDRGIRVSYGAPAPSGSVSGLR
QVLPLLPRPVGT

>putative oxidoreductase [oxidoreductase]
ATGAGCGGCGGCGCGGAGCGCACGGAGGTCCTGGTCGTCGGCAGCGGGTTCGGCGGCGCG
ATCAGCGCCTACCACCTCGCAGCCGGGGGCGCGAAGGTGACCGTACTGGAGCGCGGTCCC
TGGCTGGAACACGGCCAGTTCGAGCACGACTACAAGCTCGGCTCCTCCTACACGAGGATC
TTCGACTTCGTGGTCGGCGACGGGATGAGCGTGCTGGGCGGGAACTGTGTTGGCGGGGGC
AGCGTGGTCTACTTCGCCGCCATGCCGCGCGCACCGCGCTTCGTCTTCGACCGGAAGGGC
TCCATCGGCCGGCGCATGTGGCCGAAGTCGATCAGTCGCAACACCCTTGACCCTTGGTAC
GACCGTGTCGAGGAGTCACTGTCGGTCGCCCGGCAGGGCTGGCAGGACGTGAGCTACGCC
GGCGGCCTGTGGGCTGCAGCATGCTCCCACGCGGGCCGCACGGCCAACCCTCTCCCGGTG
GCCATCGACACCGTCAAGTGTGTCAACTGCAACTGGATGATGGCCGGGTGCCGCTTCGAG
GCCAAGCAGTCGCTGCTCGTGAACTACCTCCCCGCAGCCCTCGCCCATGGCGCCGAGATC
CGTCCCCTGCACGAGGTCCAGTACCTGGAGCGGACCGCGGACGGCGGGTACCGCGTGCAC
TACAACGTCATCGACGGCGAGGACTACCGCACGACCGCCGGCAGTGGGGTGATCGAGGCG
AAGATCGTCGTGTTGGCGGCCGGTGCCGGGGCGACGCCGGTGATCCTGCAGCGCAGCGAA
GGACAGCTCGGCCGGATGCCCCATGCGGTGGGACGCTACTTCTCGGGCAACGGCGAACGG
CTCAACACTGCCGTGGTGGACGAAGACAAGGCGGCCGAACTGCTCGGCCTGGACCGCGGG
AGCGGGCCAGCGTACGGCGCCAACCACATCGGCCGCGGACCGACCGTCGCGAGTTGGGAC
CGCCTGGACGGCTCCCTGCCCGAGTACTCCCGTTACTCGCTGGAGCAGCTCTACTTTCCA
CCCGGCCTGGGCACGATCCTCGCCCAAGTGCCGCACGGCACCGGCCCCACCTGGTTCGGC
GCCCGGAAGAAGGAGATCCTCAAACGGTGGACCTCCTGGCTGACGGTCTTCTCGATGATC
GAGGACGACAACGAGGGAGTTTTCGGCCCACCGCCAGCGACGGGCAACGCCCACCGTATC
TCGCAGCAGTTGCTCGGCCGCGGCAACCTCCGGTACGCGCCCACGCAGAACACGCTCAAC
GCCTGGGCCGCCTCCGACGCGGAGGTAAAGGAGATCCTTGAGAAGGACGGACTGGCCCAG
GTGATGCCTTGGACCAACGACCTGGTCGGCGCATACACCGTGCACCCCCTCGCCTCCTGC
CGGATGGGGGACGATCCGCACACGTCCGCTCTCGACGACCGCAACGAGCTGCGGGGGCAC
CCAGGGATCTTCGTCACGGACGGGTCCGCCGTTCCCGGTGCGCTGACCGTCAACCCGGCC
CTGACCATCTCGGCCCTCGCCGAACGGGCCGTGCCGGGCATCGTCGAGGCTGCGCAGGAC
CGTGGCATCCGCGTCAGCTACGGCGCGCCGGCGCCCAGCGGCTCCGTGAGCGGTCTGCGG
CAGGTCCTTCCGCTGCTGCCCCGCCCCGTGGGCACCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000172 Glucose-methanol-choline oxidoreductase, N-terminal (Domain) PF00732 GMC_oxred_N IPR007867 Glucose-methanol-choline oxidoreductase, C-terminal (Domain) PF05199 GMC_oxred_C
SignalP	Eukaryota
TMHMM	No significant hit

Ncarz_00250 Ncarz_00270