Ncarz_00290 Ncarz_00290   Ncarz_00310 Ncarz_00310

Ncarz_00300 : CDS information

close this sectionLocation

Organism
StrainATCC 15944
Entry nameNeocarzinostatin
Contig
Start / Stop / Direction51,551 / 50,199 / - [in whole cluster]
51,551 / 50,199 / - [in contig]
Locationcomplement(50199..51551) [in whole cluster]
complement(50199..51551) [in contig]
TypeCDS
Length1,353 bp (450 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative cytochrome P450
Product (GenBank)cytochrome P-450
GenencsE7
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[15797213] The neocarzinostatin biosynthetic gene cluster from Streptomyces carzinostaticus ATCC 15944 involving two iterative type I polyketide synthases. (Chem Biol. , 2005)
comment
Neocarzinostatin生合成遺伝子クラスターの報告。

ncsE7: P-450 hydroxylase

ncsE-ncsE11とncsF1-ncsF2が、NCS enediyne core biosynthesisに関与するとしている。

このORFについて機能解析されていない。
Related Reference
ACC
C9K1X7
PmId
comment
Streptomyces melanosporofaciens MI614-43F2
P450

Cyclooctatin biosynthetic gene
cotB3, cotB4がcytochromes P450としている。

cyclooctat-9-en-7-ol
↓(cotB3)
cyclooctat-9-en-5,7-diol
↓(cotB4)
cyclooctatin の反応経路を提案。

中間体Cyclooctat-9-en-7-ol、Cyclooctat-9-en-5,7-diolを精製し確認している。酵素学的な解析はしていない。
CotB4は最終産物cyclooctatinを産生するためにC-18でcyclooctat-9-en-7-olのhydroxylationを触媒すると示唆した。
ACC
P15129
PmId
[2229008] ( , )
comment
Rat
Cytochrome P450 4B1_cyp4b1

ラット肺由来のP450を精製ならびに活性を確認。

close this sectionSequence

selected fasta
>putative cytochrome P450 [cytochrome P-450]
MNGRRPVSPPLRALPGLLRKLAVDRLGMMRDAAALDDAVLVSMGPKKLFVFNRPDYAKHV
LADNAANYRKGIGLIESRKMLGDGLLTSEGELWREQRRTVQPAFRPARVAAQADAVAEET
MNLRDLLMRRGADGPVDVLQEVTGFTLGVLGRTVLNTDLGGYGGIAHAFEAVQDQAMFDM
VTQNMVPTWAPLATQRRFRRARRELIRTVDELVADRSARMTDGEEADDAFSLMIAAARRQ
TDPRTGQGRLRDELVTLLLAGHETTASTLAWTLLLLARHPHMRDLVREEARGVLADGRAP
DAGDLRKLTYTTQVVQEAMRLYPPVWILPRVARQSDEVGPYSVSAGADVLICPYTLHRHP
DLWERPEQFDPGRFDPARVADRPRYAYIPFGAGPRFCVGSNLGMMEAVFVTALLTRDLVL
EVVPGDERTPEPMMSLRMRGGLPMTVRPVR
selected fasta
>putative cytochrome P450 [cytochrome P-450]
ATGAATGGGCGGCGTCCGGTCTCCCCTCCGCTGCGGGCGCTACCCGGCCTGTTGCGCAAG
CTCGCCGTCGACCGGCTCGGGATGATGCGTGACGCGGCGGCGCTGGACGATGCTGTGCTG
GTCTCCATGGGGCCGAAGAAGCTGTTCGTCTTCAACCGGCCCGACTACGCGAAGCACGTG
CTGGCGGACAACGCCGCCAACTATCGAAAGGGCATCGGTCTGATCGAGTCCCGGAAGATG
CTCGGCGACGGTCTCCTGACGAGCGAGGGTGAACTGTGGCGCGAGCAGCGGCGCACGGTG
CAGCCAGCGTTCAGGCCGGCGCGCGTGGCCGCTCAGGCCGACGCGGTGGCGGAAGAGACC
ATGAACCTTCGGGACCTGCTGATGCGACGGGGCGCCGACGGACCCGTCGACGTACTCCAG
GAGGTGACTGGGTTCACGCTCGGTGTGCTGGGCCGGACCGTGCTCAACACCGACCTCGGG
GGGTACGGGGGCATCGCCCACGCCTTCGAAGCGGTACAGGACCAGGCGATGTTCGACATG
GTCACCCAGAACATGGTGCCGACGTGGGCTCCGCTGGCCACTCAGCGGCGGTTCCGAAGA
GCCCGCCGGGAACTCATACGGACCGTTGATGAACTGGTGGCCGACCGCAGCGCTCGCATG
ACCGACGGAGAGGAGGCCGACGACGCGTTCTCTCTCATGATTGCGGCTGCTCGCCGGCAG
ACCGATCCGAGGACCGGGCAGGGGCGGCTGCGCGACGAACTGGTGACGCTGCTCCTCGCC
GGGCACGAGACTACGGCGAGCACACTGGCGTGGACCTTGCTACTGCTTGCCCGTCATCCG
CACATGAGGGATCTGGTGCGCGAGGAGGCCCGCGGAGTGCTTGCGGACGGCCGGGCCCCG
GACGCCGGGGACCTGCGCAAACTGACTTACACGACGCAGGTCGTCCAGGAGGCCATGCGT
CTGTATCCGCCGGTGTGGATTTTGCCTCGGGTGGCCCGGCAGTCCGACGAGGTGGGACCG
TACTCGGTGTCGGCCGGCGCCGACGTCCTGATCTGCCCGTACACCCTGCACCGTCATCCG
GATCTGTGGGAGCGGCCGGAACAGTTCGACCCAGGGCGGTTCGACCCGGCGCGCGTCGCC
GATCGCCCTCGCTACGCCTACATCCCATTCGGCGCCGGTCCCCGCTTCTGTGTCGGCAGC
AACCTGGGGATGATGGAGGCGGTCTTCGTTACCGCCCTCCTGACACGGGATCTCGTCCTG
GAGGTCGTGCCCGGCGATGAGAGGACGCCCGAGCCGATGATGTCCCTGCGCATGCGGGGC
GGGCTTCCCATGACGGTTCGGCCTGTGCGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[3-448] 3.10001307370203e-106 SSF48264
SSF48264   Cytochrome_P450
[260-277] 4.19999771339581e-15 PR00385 [313-324] 4.19999771339581e-15 PR00385 [388-397] 4.19999771339581e-15 PR00385 [397-408] 4.19999771339581e-15 PR00385
PR00385   P450
[37-432] 1.79999999999997e-76 PF00067
PF00067   p450
[8-448] 5.40000000000002e-106 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
IPR002401 Cytochrome P450, E-class, group I (Family)
[249-266] 2.60000517657733e-18 PR00463 [269-295] 2.60000517657733e-18 PR00463 [352-376] 2.60000517657733e-18 PR00463 [387-397] 2.60000517657733e-18 PR00463 [397-420] 2.60000517657733e-18 PR00463
PR00463   EP450I
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[390-399] PS00086
PS00086   CYTOCHROME_P450
SignalP
[1-13] 0.48 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Ncarz_00290 Ncarz_00290   Ncarz_00310 Ncarz_00310
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