Ncarz_00280 Ncarz_00280   Ncarz_00300 Ncarz_00300

Ncarz_00290 : CDS information

close this sectionLocation

Organism
StrainATCC 15944
Entry nameNeocarzinostatin
Contig
Start / Stop / Direction49,345 / 50,139 / + [in whole cluster]
49,345 / 50,139 / + [in contig]
Location49345..50139 [in whole cluster]
49345..50139 [in contig]
TypeCDS
Length795 bp (264 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative AraC family transcriptional regulator
Product (GenBank)transcriptional regulator
GenencsR5
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[15797213] The neocarzinostatin biosynthetic gene cluster from Streptomyces carzinostaticus ATCC 15944 involving two iterative type I polyketide synthases. (Chem Biol. , 2005)
comment
Neocarzinostatin生合成遺伝子クラスターの報告。

ncsR5: AraC family, transcriptional regulator

このORFについて機能解析されていない。
Related Reference
ACC
Q8GME4
NITE
C1027_00490
PmId
[19159491] Role of sgcR3 in positive regulation of enediyne antibiotic C-1027 production of Streptomyces globisporus C-1027. (BMC Microbiol. , 2009)
[20551990] Manipulation of pathway regulation in Streptomyces globisporus for overproduction of the enediyne antitumor antibiotic C-1027. (J Antibiot (Tokyo). , 2010)
comment
Streptomyces globisporus
Putative regulatory protein

[PMID:19159491]
C-1027の生合成遺伝子SgcR2。
制御遺伝子として同定されたSgcR1,R2,R3のSgcR3に関しての機能解析論文。

sgcR3過剰発現株はC-1027の産生能が上がり、sgcR3欠損株はC-1027が産生されなかった。
また、sgcR3欠損株の転写解析ではsgcA1やsgcC4のような生合成構造遺伝子だけでなく、制御遺伝子sgcR1,sgcR2の転写量も減少した。cross-complementation studyにて、sgcR3がsgcR1R2のプロモーター領域に直接結合することでsgcR1とsgcR2の転写を活性化した。


[PMID:20551990]
SgcR1,SgcR2の機能解析論文。

sgcR1,sgcR2,sgcR3の過剰発現株を各作製し、これらの株におけるC-1027とheptaene産生の変化および生物活性を見ている。
C-1027の生産にpositive activatorとして機能していることが示された。

close this sectionSequence

selected fasta
>putative AraC family transcriptional regulator [transcriptional regulator]
MTSATIEHAVHRVISNMRANLGQDLTIDDMARTAMFSKFHFTRIFRDVTGTSPGRFLSAL
RIQEAKRLLVQTQFSVADISSLVGYSSVGTFSSRFKACVGVSPSVYRELEGFATGIDQEP
WPDSPTSPAGVSGITLWGHVTLPEGRAPGPCYVGLFPTQVPQGRPVRCSVLEGPGPFELR
NVPVGTWYLLVHSVPYGQEHLVSGAAGDEDSDTVSVGRHGPLTVRAETVPLPVSVALRPL
EVFDPPVLMALLDLRTGTLASATG
selected fasta
>putative AraC family transcriptional regulator [transcriptional regulator]
ATGACTTCCGCTACCATCGAGCACGCAGTTCACCGGGTCATCAGCAATATGCGGGCCAAT
CTCGGGCAAGACCTGACGATCGACGACATGGCACGCACCGCTATGTTCAGTAAATTCCAC
TTCACTCGTATATTCAGGGACGTGACCGGCACCTCGCCCGGCAGGTTCCTTTCGGCTCTG
CGCATCCAGGAGGCCAAGCGCCTGCTCGTGCAGACGCAGTTCAGCGTCGCGGACATCAGC
AGCCTGGTCGGCTACAGCAGCGTCGGCACCTTCAGCTCGCGCTTCAAGGCATGTGTGGGC
GTCTCCCCGAGCGTCTACCGCGAACTGGAGGGCTTCGCCACCGGCATCGACCAGGAACCG
TGGCCGGACTCCCCCACCTCCCCGGCCGGCGTCTCCGGCATCACTCTGTGGGGCCACGTC
ACGCTTCCCGAGGGGCGTGCCCCAGGCCCTTGTTACGTCGGCCTGTTTCCCACCCAGGTT
CCTCAGGGGCGGCCGGTGAGATGCTCCGTCCTGGAGGGACCCGGCCCCTTCGAACTGCGC
AACGTTCCCGTCGGCACCTGGTACCTCCTGGTGCACTCGGTGCCCTACGGCCAGGAGCAC
CTCGTCTCCGGCGCCGCCGGTGACGAAGACAGCGACACCGTTTCCGTCGGACGCCACGGC
CCGCTGACGGTGCGGGCCGAGACAGTGCCCCTGCCGGTCAGTGTCGCGCTGCGACCGCTG
GAGGTCTTCGACCCGCCGGTGCTGATGGCACTGCTCGACCTGCGCACCGGCACCCTGGCG
AGCGCCACGGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR009057 Homeodomain-like (Domain)
[6-57] 1.29999924468179e-10 SSF46689 [60-108] 3.1999989904635e-13 SSF46689
SSF46689   Homeodomain_like
[8-60] 1.3e-22 G3DSA:1.10.10.60 [61-108] 6.1e-20 G3DSA:1.10.10.60
G3DSA:1.10.10.60   Homeodomain-rel
IPR018060 DNA binding HTH domain, AraC-type (Domain)
[24-107] 3.89999861795218e-33 SM00342
SM00342   HTH_ARAC
[11-109] PS01124
PS01124   HTH_ARAC_FAMILY_2
IPR018062 HTH domain AraC-type, conserved site (Conserved_site)
[61-103] PS00041
PS00041   HTH_ARAC_FAMILY_1
IPR020449 Transcription regulator HTH, AraC- type (Family)
[76-91] 3.30000164480624e-05 PR00032 [91-107] 3.30000164480624e-05 PR00032
PR00032   HTHARAC
SignalP No significant hit
TMHMM No significant hit
Ncarz_00280 Ncarz_00280   Ncarz_00300 Ncarz_00300
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