Niger_00070 Niger_00070   Niger_00090 Niger_00090

Niger_00080 : CDS information

close this sectionLocation

Organism
StrainDSM 4137
Entry nameNigericin
Contig
Start / Stop / Direction58,197 / 57,331 / - [in whole cluster]
58,197 / 57,331 / - [in contig]
Locationcomplement(57331..58197) [in whole cluster]
complement(57331..58197) [in contig]
TypeCDS
Length867 bp (288 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.4 Other
Productputative thioesterase
Product (GenBank)NigCII
Gene
Gene (GenBank)nigCII
EC number
Keyword
  • unusual type
Note
Note (GenBank)
  • putative thioesterase; ORF2
Reference
ACC
PmId
[17584617] Insights into polyether biosynthesis from analysis of the nigericin biosynthetic gene cluster in Streptomyces sp. DSM4137. (Chem Biol. , 2007)
comment
Streptomyces sp. DSM4137におけるNigericin Biosynthetic Gene Clusterに関する文献。

nigCII (288 aa): thioesterase

nigCII 自体の機能実験は行われておらず、monesin biosynthesis (Streptomyces cinnamonensis)のmonCII との相同性よりthioesteraseと予想している。
Related Reference
ACC
Q7WTE2
NITE
Nanch_00210
PmId
[16984884] Identification of NanE as the thioesterase for polyether chain release in nanchangmycin biosynthesis. (Chem Biol. , 2006)
[18482697] Mechanism of thioesterase-catalyzed chain release in the biosynthesis of the polyether antibiotic nanchangmycin. (Chem Biol. , 2008)
comment
2nd(Q7WTE2) 50%, 6e-69
Streptomyces nanchangensis_nanE
NanE
[Nanch_00210]thioesterase

---
[PMID: 16984884](2006)
recombinant CR domain, ACP-CR didomain, NanEがテストされたとき、NanEだけがpolyether-SNACを加水分解した。よってNanEがpolyketide鎖(polyether鎖)の放出を担うthioesteraseである。

CR(chain-releasing) domainは、nanchangmycin PKSの最終moduleにあるACP14の下流に融合したdomain.

---
[PMID: 18482697](2008)
glycosyl transferase gene nanG5のdeletionもnanchangmycin生合成を廃止することから、NanEはnanchangmycin生合成における最終ステップを触媒することを強く示す。
NanEのcatalytic triadの同定もしている。
ACC
Q846Y1
NITE
Monen_00100
PmId
[12940979] Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization. (Mol Microbiol. , 2003)
[16897798] Evidence that a novel thioesterase is responsible for polyketide chain release during biosynthesis of the polyether ionophore monensin. (Chembiochem. , 2006)
comment
3rd(Q846Y1) 47%, 1e-60
Streptomyces cinnamonensis_monCII
Monensin cyclase
[Monen_00100]thioesterase

---
[PMID: 12940979](2003)
monensin生合成 gene clusterの解析論文。

MonCII: Cyclase
monCII のTargeted gene replacementによってmonensin非産生。

---
[PMID: 16897798](2006) abstract
epoxide hydrolaseであると考えられてきたが、alpha/beta-hydrolase structural familyに属する新しいthioesteraseである。

精製されたrecombinant MonCII は、monensin AのN-acetylcysteamine thioester derivativeを含むいくつかのthioester基質を加水分解する。
phenylmethanesulfonyl fluorideのような酵素のhallmark inhibitorとのincubationは、thioesterase活性の抑制と、MonCII のacylated formの蓄積を導く。

close this sectionSequence

selected fasta
>putative thioesterase [NigCII]
MDLSVPVTDDVKLHFRHRPGTASLPFLLVHGMASSARLWDEVADHLAAAGHAVYAADLRG
HGDSDTPETGYDTPTAVADLVAAAAALSLDRVVVAGHSWGGNVSVRLTAEHPELVAALAL
VDGGWLEPAKAFSSWDAFVGALRPASLEGATEQSVSDYFRAIHPDWSPAAIEAAVATMVV
NPDGSLSRRMSTEQHMSILHSIWNEPPRPWYAAIDVPTLLMPAIPKGADEKWADRIRSRI
KETTADLRHATMREYLDSEHDLHAQHPRRVADDLLDLAHCVRPAHSAR
selected fasta
>putative thioesterase [NigCII]
ATGGATCTTTCCGTGCCCGTCACCGACGATGTGAAATTGCATTTCCGGCACCGGCCGGGA
ACGGCGTCCCTTCCCTTTCTCCTGGTCCATGGCATGGCGTCGAGCGCCCGGCTGTGGGAC
GAGGTCGCCGACCATCTGGCCGCCGCGGGCCATGCGGTGTACGCGGCGGATCTGCGCGGC
CACGGGGACTCCGACACCCCGGAGACCGGATACGACACCCCGACCGCGGTCGCGGATCTG
GTGGCGGCGGCCGCCGCCCTAAGTCTTGACCGGGTGGTGGTGGCCGGTCACTCCTGGGGC
GGCAATGTCTCGGTGCGGCTGACCGCCGAGCACCCCGAACTGGTGGCCGCGCTGGCCCTC
GTGGACGGCGGCTGGCTGGAGCCGGCCAAGGCGTTCTCCTCCTGGGACGCGTTCGTCGGG
GCGTTGCGGCCGGCGTCCCTGGAGGGCGCCACCGAGCAGAGCGTGAGCGACTATTTCCGG
GCCATCCATCCGGACTGGTCCCCGGCGGCGATCGAGGCCGCGGTGGCCACGATGGTGGTC
AACCCGGACGGTTCGCTGTCCCGGCGCATGTCCACCGAACAGCACATGTCGATCCTGCAC
AGCATCTGGAACGAGCCGCCGAGGCCGTGGTACGCGGCCATCGACGTGCCCACCCTGCTG
ATGCCCGCCATTCCCAAGGGCGCGGACGAGAAGTGGGCGGACCGCATCCGCTCCCGCATC
AAGGAGACCACGGCCGACCTGCGCCACGCGACGATGCGTGAGTATCTCGACTCCGAGCAC
GATCTGCACGCCCAGCACCCACGGCGGGTGGCGGACGATCTGCTCGACCTGGCCCACTGC
GTTCGGCCCGCCCACAGCGCTCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000073 Alpha/beta hydrolase fold-1 (Domain)
[50-65] 1e-06 PR00111 [94-107] 1e-06 PR00111 [108-121] 1e-06 PR00111
PR00111   ABHYDROLASE
IPR000639 Epoxide hydrolase-like (Family)
[30-48] 2.19999900980708e-05 PR00412 [50-65] 2.19999900980708e-05 PR00412 [94-107] 2.19999900980708e-05 PR00412 [255-277] 2.19999900980708e-05 PR00412
PR00412   EPOXHYDRLASE
SignalP
[1-35] 0.467 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Niger_00070 Niger_00070   Niger_00090 Niger_00090
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